NOP14过表达抑制NRIP1表达及卵巢癌SKOV3细胞的增殖与迁移

doi:10.3870/j.issn.1672-8009.2026.01.009

摘要/Abstract

摘要： 目的探究核仁蛋白14(nucleolar protein 14,NOP14)过表达对卵巢癌SKOV3细胞增殖与迁移的影响及机制。方法检测卵巢癌细胞系(SKOV3、A2780、HO-8910、OVCAR)中NOP14的表达水平。SKOV3细胞中转染pcDNA-NOP14质粒以构建NOP14过表达细胞系。细胞克隆形成实验和5-乙炔基-2′-脱氧尿苷(5-Ethynyl-2′-deoxyuridine,EdU)染色检测细胞增殖能力。无血清成球培养分析肿瘤干细胞特性。流式细胞术检测CD133阳性细胞比例。细胞形态学观察及上皮-间质转化(epithelial-mesenchymal transition,EMT)标志蛋白(E-cadherin、N-cadherin、Vimentin)检测评估EMT进程。蛋白质印迹和RT-qPCR检测核受体相互作用蛋白1(nuclear receptor interacting protein 1,NRIP1)及Wnt、β-catenin的表达水平。结果在卵巢癌细胞系中NOP14 mRNA和蛋白表达均显著低于正常卵巢上皮细胞。NOP14过表达后抑制SKOV3细胞增殖、干细胞特性及EMT转化(间质标志物N-cadherin、Vimentin下调,上皮标志物E-cadherin上调),以及NRIP1、Wnt和β-catenin的表达水平。结论 NOP14可负向调控NRIP1和Wnt及β-catenin的表达,并抑制卵巢癌SKOV3细胞的增殖、干细胞特性及EMT进程。

关键词: 核仁蛋白14, 卵巢癌, 核受体相互作用蛋白1, 细胞增殖, 上皮-间质转化

Abstract: Objective To investigate the effect of nucleolar protein 14(NOP14)overexpressi on proliferation and migration of ovarian cancer cells SKOV3 and the underlying mechanism. Methods The expression level of NOP14 in ovarian cancer cell lines(SKOV3,A2780,HO-8910,OVCAR)was detected by RT-qPCR and Western blotting.SKOV3 cells were transfected with the pcDNA-NOP14 plasmid.Cell proliferation was detected by colony formation assay and 5-Ethynyl-2′-deoxyuridine(EdU)staining.Tumor stem cell characteristics were analyzed via serum-free sphere formation assay.The proportion of CD133-positive(CD133⁺)cells was measured by flow cytometry.The epithelial-mesenchymal transition(EMT)process was evaluated by observing cell morphological change and detecting the EMT marker proteins(E-cadherin,N-cadherin,Vimentin).The expression level of nuclear receptor interacting protein 1(NRIP1),and Wnt/β-catenin pathway proteins were detected by RT-qPCR and Western blotting. Results The expression level of NOP14 was significantly lower in the ovarian cancer cell lines than that in the normal ovarian epithelial cells.Overexpression of NOP14 significantly inhibited the proliferation,stem cell characteristics,and EMT of SKOV3 cells(mesenchymal markers N-cadherin and Vimentin were downregulated,while the epithelial marker E-cadherin was upregulated).Moreover,Overexpression of NOP14 suppressed the expression of NRIP1,Wnt and β-catenin. Conclusion NOP14 can inhibit the proliferation,stem cell characteristics,and EMT process of ovarian cancer SKOV3 cells,and negatively regulate the expression of NRIP1,Wnt,β-catenin.

Key words: nucleolar protein 14, ovarian cancer, nuclear receptor interacting protein 1, cell proliferation, epithelial-mesenchymal transition

中图分类号:

R349.5

李苓妙, 李萍, 胡晓丽, 王冬梅, 武子先. NOP14过表达抑制NRIP1表达及卵巢癌SKOV3细胞的增殖与迁移[J]. 医学分子生物学杂志, 2026, 23(1): 67-73.

LI Lingmiao, LI Ping, HU Xiaoli, WANG Dongmei, WU Zixian. NOP14 Overexpression Inhibits NRIP1 Expression and Proliferation and Migration of Ovarian Cancer SKOV3 Cells[J]. Journal of Medical Molecular Biology, 2026, 23(1): 67-73.

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