Office
Journal
Current Issue
31 May 2025, Volume 22 Issue 3
Previous Issue   
Protective Effect of Dihydrolycorine on Rats with CVB3-induced Viral Myocarditis #br#
BAI Hongmin, YANG Yongzhong, CHEN Shujie, YANG Chao, LIU Wei
2025, 22(3):  211-216.  doi:10.3870/j.issn.1672-8009.2025.03.001
Abstract ( 23 )   PDF (7677KB) ( 11 )  
Related Articles | Metrics
Objective To explore the influence and mechanism of dihydrolycorine ( DL) onrats with viral myocarditis (VMC) induced by Coxsackie B3 virus (CVB3). Methods A total of72 SD rats were randomized into 6 groups: blank control group, model group, 10 mg / kg DLgroup, 20 mg / kg DL group, 40 mg / kg DL group, and Shenmai injection group (200 mg / kg),with 12 rats in each group. The CVB3-induced VMC model was established and intervened for 7days. The levels of serum myocardial enzymes and the hemodynamic indexes were detected. HE staining was used to detect the myocardial tissue pathological damage. TUNEL was used to detect myocardial cell apoptosis. Western blotting was applied to detect the expression levels of apoptosis andendoplasmic reticulum stress-related proteins. Results Compared with those in the model group,the pathological damage of myocardial tissue was alleviated, and the levels of serum myoglobin(Mb), creatine kinase isoenzyme MB (CK-MB) and cardiac troponin I (cTnI), the myocardialapoptosis rate and the protein expression levels of BCL2-associated X protein (Bax), C / EBP homologous protein (CHOP), glucose-regulated protein 78 ( GRP78) and GRP94 were reduced inthe 20 and 40 mg / kg DL groups and the Shenmai injection group ( P < 0. 05). Moreover, the leftventricular pressure (LVP), the maximum change rate of left ventricular pressure rise and fall ( ±dp / dtmax), and the protein expression levels of B-cell lymphoma-2 ( Bcl-2) and the cleaved /caspase-3 ratio rose in the 20 and 40 mg / kg DL groups and the Shenmai injection group ( P <0. 05). Conclusion DL can relieve the VMC myocardial injury and improve the cardiac dysfunction, which may be related to the inhibition of endoplasmic reticulum stress-mediated apoptosis.

Effect of Olaparib on Survival, Epithelial-mesenchymal Transition, and Tumor Stem Cell-like Characteristics in Ovarian Cancer ES2 Cells #br#
YE Jing, ZHAO Jinrong , LI Ping, HAN Ci, ZHANG Xin, CHENG Wei, TIAN Xiaoru
2025, 22(3):  217-222.  doi:10.3870/j.issn.1672-8009.2025.03.002
Abstract ( 12 )   PDF (4429KB) ( 8 )  
Related Articles | Metrics
Objective To explore the effect of olaparib on the survival, epithelial-mesenchymal transition ( EMT ) and tumor stem cell-like characteristics of ovarian cancer ES2cells. Methods Ovarian cancer ES2 cells were culturedin vitro, and they were divided into 5groups: control group, low-dose olaparib group, middle-dose olaparib group, high-dose olaparib group and doxorubicin 0. 5 μmol / L group. Cells proliferation, apoptosis, invasion and migration were detected by 5-ethynyl-2′-deoxyuridine ( EDU) staining, flow cytometry, Transwell assay, and wound healing assay, respectively. The epithelial-mesenchymal transition ( EMT) of cells was observed under light microscope, tumor stem cell-like characteristics were observed by tumor spheroid formation assay, and protein expression level was detected by Western blotting. Results Compared with those in the control group, the apoptosis rate of ES2 cells and the expression level of Ecadherin were significantly increased in the low-, middle-, high-dose olaparib group and doxorubicin 0. 5 μmol / L group ( P < 0. 05), the ratio of EDU staining positive cells, number of invasioncells per unit area, cell wound-healing rate, number of tumor spheroid, spheroid diameter, and the expression levels of nuclear antigen Ki67, Survivin, vascular endothelial growth factor(VEGF), N-cadherin, cluster of differentiation 44 (CD44) and aldehyde dehydrogenase 1 (ALDH1) were significantly decreased in the middle-and high-dose olaparib group and doxorubicin 0. 5μmol / L group (P< 0. 05). Conclusion Olaparib of over 30 mg / L can significantly inhibit proliferation, invasion, and migration of ovarian cancer ES2 cells, and promote their apoptosis, which may be through inhibition of Ki67 and regulation of EMT.

CircITCH Regulates Proliferation and Invasion of Colorectal Cancer Cells via Targeting miR-106b #br#
JIANG Linyan, SHEN Xiaowen, XU Meirong
2025, 22(3):  223-228.  doi:10.3870/j.issn.1672-8009.2025.03.003
Abstract ( 13 )   PDF (2758KB) ( 6 )  
Related Articles | Metrics
Objective To study the effect of circITCH on the proliferation, invasion of colorectal cancer cells via targeting miR-106b. Methods Colorectal cancer cell lines SW620, HT-29,HCT116, Caco-2, SW480, and normal colon epithelial cell line NCM460 were cultured. The expression levels of circITCH and miR-106b were detected, and HT-29 cells were selected for further experiments and were divided into 7 groups: Negative control ( NC) group, circITCH group, miR-NC group, miR-106b group, NC plasmid + miR-NC group, circITCH + miR-NC group, andcircITCH + miR-106b group. The A450 value, number of invasion cells and the expression levels of PTEN and B cell translocation gene 3 (BTG3) were detected. Results The expression level of circITCH in the SW620, HT-29, HCT116, Caco-2 and SW480 cells was lower than that in theNCM460 cell, while the expression level of miR-106b was higher in the NCM460 cell (P < 0. 05).Dual-luciferase reporter gene assay showed that miR-106b could bind with circITCH reporter gene). The expression levels of circITCH, PTEN, BTG3 in the circITCH group were higher than those inthe NC group, the expression level of miR-106b, A450 value, and the number of invasion cells were lower than those in the NC group (P < 0. 05). The expression level of miR-106b, A450 value, andthe number of invasion cells in the circITCH + miR-106b group were higher than those in the circITCH + miR-NC group, the expression levels of PTEN, BTG3 were lower than those in the circITCH + miR-NC group (P< 0. 05). Conclusion CircITCH can inhibit the proliferation and invasion of colorectal cancer cells via targeting miR-106b.

Effect of Nrf2 on Brain Injury and Microglial Cell Polarization in Neonatal Hypoxic-ischemic Encephalopathy Rats #br#
WU Zhishan, XIAO Pei, LI Yanliang, YU Zhanzhang
2025, 22(3):  229-236.  doi:10.3870/j.issn.1672-8009.2025.03.004
Abstract ( 15 )   PDF (8862KB) ( 8 )  
Related Articles | Metrics
Objective To investigate the effect of nuclear factor E2-associated factor 2 (Nrf2)on brain injury in neonatal hypoxic-ischemic encephalopathy ( HIE) rats. Methods The experiments were divided into 4 groupd: Sham group, HIE group, Sham + Nrf2 group, and HIE + Nrf2group, with 10 neonatal SD rats in each group. The HIE model was established, and unloaded adenovirus or adenovirus containing Nrf2 overexpression vector was transfused into the ventricle. After 14days, cerebral infarction was detected by 2, 3, 5 triphenyltetrazolium chloride ( TTC) staining, and brain histopathological changes were observed by Hematoxylin-eosin (HE) staining. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β ( IL-1β), interleukin-6 (IL-6), and interleukin-10 ( IL-10 ) in brain tissues were determined by enzyme-linked immunosorbent assay (ELISA). The proportion of M1-type microglia labeled with ionized calcium binding adaptor molecule-1 (Iba-1) / inducible nitric oxide synthetase (iNOS) and the proportion of M2-type microglia labeled with Iba-1 / arginase 1 ( Arg-1) were detected by double-labeling immunofluorescence staining. The protein expression levels of iNOS, CD86, Arg-1, Macrophage mannose receptor(CD206), Nrf2, brain-derived neurotrophic factor (BDNF), and glia-derived neurotrophic factor(GDNF) were detected by Western blotting. Results Compared with those in the HIE group, thevolume of cerebral infarction in the HIE + Nrf2 group was significantly decreased, neuronal nucleus shrinkage was decreased, the levels of TNF-α, IL-1β and IL-6 in brain tissues were significantly decreased, and the levels of IL-10 were significantly increased, the proportion of Iba-1 + / iNOS + M1- type microglia was significantly decreased, and the proportion of Iba-1 + / Arg-1 + M2-type microglia was significantly increased, the expression levels of iNOS and CD86 protein in brain tissues were significantly down-regulated, the expression levels of Arg-1, CD206, Nrf2, BDNF and GDNF were significantly up-regulated (P < 0. 05). Conclusion Nrf2 can reduce neuroinflammation by promotingthe transformation of microglia from M1 type to M2 type to improve the brain injury of neonatal rats with HIE.

Vitamin D3 Mediates TIPE2 in Regulation of Macrophage Function and Phenotype in Chronic Rhinosinusitis with Nasal Polyps #br#
HUANG Xiaorong, TAN Hui, CHEN Juan, Kadiliya Mulati, ZHANG Jin, CHEN Lunjian
2025, 22(3):  234-244.  doi:10.3870/j.issn.1672-8009.2025.03.005
Abstract ( 12 )   PDF (1663KB) ( 6 )  
Related Articles | Metrics
Objective To investigate the regulatory role of vitamin D3 ( VD3 ) -mediatedtumor necrosis factor-alpha induced protein 8-like protein 2 ( TIPE2) on macrophage function andphenotype in chronic rhinosinusitis with nasal polyps (CRSwNP). Methods Peripheral blood samples were collected from patients with CRSwNP as the CRSwNP group, and from patients under went surgery for isolated nasal septal deviation as the Control group. RT-qPCR and Western blotting were used to detect the expression levels of TIPE2 and Arginase-1 ( Arg-1) in peripheral blood, andchemiluminescence was used to detect the changes in VD3 concentration in peripheralblood. J774A. 1 macrophages were cultured in vitro and divided into 5 groups: silenced TIPE2 (siTIPE2#2) group and its negative control ( si-NC) group, negative control for TIPE2 overexpression (oe-NC) group, oe-NC combined with VD3 treatment group ( oe-NC + VD3 group), and overexpressed TIPE2 recombinant vector ( oe-TIPE2 ) combined with VD3 treatment group ( oeTIPE2 + VD3 group). In addition, a mouse model of CRSwNP was established, with CRSwNP mice divided into 3 groups: empty vector treatment group (CRSwNP + empty vector group), CRSwNP + oeTIPE2 group, and CRSwNP + oe-TIPE2 + VD3 group. Peripheral blood and macrophages were collected fr om each group of mice. RT-qPCR and Western blotting were used to detect the expression levels of TIPE2 and Arg-1 in macrophages. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of interleukin ( IL ) -4 and IL-13 in cell culture supernatants and mouse sera in eachgroup. Results Compared to those in the control group, VD3 concentration was decreased in the CRSwNP group, while the expression levels of TIPE2 and Arg-1, as well as the serum concentrations of IL-4and IL-13, were upregulated (all P< 0. 05). There was a significant positive correlation between Arg-1 and TIPE2 levels (r =0. 994, P<0. 01), and both Arg-1 and TIPE2 levels were significantly negatively correlated with VD3 concentration (r = -0. 652, P<0. 01; r = -0. 745, P<0. 01). Compared to thosein the si-NC group, the expression levels of Arg-1 and IL-4 and IL-13 concentrations decreased in the siTIPE2#2 group (all P< 0. 05). Compared to those in the oe-NC group, the expression levels of TIPE2 and Arg-1, as well as IL-4 and IL-13 concentrations, decreased in the oe-NC + VD3 group (all P <0. 05). Moreover, compared to those in the oe-NC + VD3 group, the expression levels of TIPE2 and Arg-1, as well as IL-4 and IL-13 concentrations, increased in the oe-TIPE2 + VD3 group (all P < 0. 05).Compared to those in the CRSwNP + emptyvector group, the expression levels of TIPE2 and Arg-1 increased in the CRSwNP + oe-TIPE2 group, while VD3 concentration decreased and the serum concentrations of IL-4 and IL-13 increased (all P<0. 05). Compared to those in the CRSwNP + oe-TIPE2 group,the expression levels of TIPE2 and Arg-1 decreased in the CRSwNP + oe-TIPE2 + VD3 group, while theVD3 concentration increased and the serum concentrations of IL-4 and IL-13 decreased (all P<0. 05).Conclusion VD3 inhibits the expression of Arg-1, IL-4, and IL-13 in CRSwNP by downregulatingTIPE2 expression.
Adipose Stem Cell-derived Exosomes Promote Osteogenic Differentiation of Periodontal Ligament Stem Cells through miR-654-3p #br#
YANG Yimi, WANG Wei, CHEN Jiedian, AN Bingtao, WEN Ying
2025, 22(3):  245-251.  doi:10.3870/j.issn.1672-8009.2025.03.006
Abstract ( 14 )   PDF (5083KB) ( 10 )  
Related Articles | Metrics
Objective To investigate the effect of adipose stem cells-derived exosomes ( ADSCs-Exo) on osteogenic differentiation of periodontal ligament stem cells ( PDLSCs) through themiR-654-3p pathway. Methods Human ADSCs were isolated, identified, and cultured in vitro,and exosomes were isolated. Human PDLSCs were isolated in vitro, and the miR-654-3p inhibitor orits negative control (inhibitor NC) were transferred into cells, and cultured with or without a combined osteogenic induction solution. Cells were divided into 6 groups: Blank group, ADSCs-Exogroup, inhibitor NC group, inhibitor NC + ADSCs-Exo group, miR-654-3p inhibitor group, miR-654-3p inhibitor + ADSCs-Exo group. After 7 days of induction, the expression levels of miR-654-3p, osteocalcin ( OCN) and typeⅠ collagen ( COL1A1) mRNA in cells were detected by RTqPCR, and ALP activity was detected in cells by alkaline phosphatase (ALP) activity kit. After 21days of induction, the alizarin red staining method was used to detect the calcium nodules’ formation ability of cells, and the expression levels of OCN and COL1A1 protein were detected by West ern blotting. Results Compared with those in the Blank group or inhibitor NC group, the expression level of miR-654-3p, the activity of ALP, the expression levels of OCN and COL1A1 mRNAand protein in the ADSCs-Exo group were increased (P< 0. 05), and the formation ability of calcium nodules was enhanced (P < 0. 05), and the expression level of miR-654-3p was decreased inthe miR-654-3p inhibitor group. Compared with those in the inhibitor NC + ADSCs-Exo group, the ALP activity, the expression levels of OCN and COL1A1 mRNA and protein in the miR-654-3p inhibitor + ADSCs-Exo group were decreased (P < 0. 05), and the formation ability of calcium nodules was decreased ( P < 0. 05 ). Conclusion ADSCs-Exo promote osteogenic differentiation of
PDLSCs by up-regulating the expression of miR-654-3p.


Wutou Decoction Inhibits Inflammation and Oxidative Stress Induced Injury of Human Urogenic Stem Cells by Ahr/ LOC101928120 / SHC1 Signaling Pathway #br#
WU Dan, TONG Liangming, LI Xi, ZHOU Ping, LI Jiefang
2025, 22(3):  252-258.  doi:10.3870/j.issn.1672-8009.2025.03.007
Abstract ( 11 )   PDF (2239KB) ( 6 )  
Related Articles | Metrics
Objective To investigate the mechanism by which Wutou decoction ( WTD) inhibits inflammatory and oxidative stress induced injury of human urogenic stem cells ( UdSC) bymodulating the aryl hydrocarbon receptor ( Ahr ) / long non-coding RNA ( LncRNA )LOC101928120 / SHC articulin 1 ( SHC1) signaling pathway. Methods UdSC cells were dividedinto 5 groups Control group, IL-1β group, IL-1β + WTD group, H2 O2 group, and H2 O2 + WTD group. CellTiterGlo assay and flow cytometry were applied to detect the proliferation and apoptosis in the cells of each group. qRT-PCR was applied to detect the expression levels of Ahr, LOC101928120, and SHC1 mRNA. Western blotting was applied to detect the expression levels of Ahr, HDAC1, SHC1, COL2, and SOX-9. ELISA was applied to detect the levels of IL-6, TNF-α, and MMP-13 in inflammation-induced UdSC cells. DCFH-DA fluorescent probe was applied todetect the ROS level in oxidative stress-induced UdSC cells. Results The A 560 nmvalue, the expression levels of Ahr mRNA and protein, LOC101928120, COL2, SOX-9 protein in the IL-1β group were lower than those in the Control group, and the values of above indexes in the IL-1β + WTDgroup were higher than those in the IL-1β group (P< 0. 05). The apoptosis rate, the levels of IL-6,TNF-α, MMP-13, and the expression levels of SHC1 mRNA and protein and HDAC1 protein in the IL-1β group were higher than those in the control group, and the values of above indexes in the IL-1β + WTD group were lower than those in the IL-1β group (P < 0. 05). TheA560 nm value, the expression levels of Ahr mRNA and protein, LOC101928120, COL2 and SOX-9 proteins in the H2O2 group were lower than those in the Control group, and the values of above indexes in the H2 O2 + WTD group were higher than those in the H2O2 group (P< 0. 05). The apoptosis rate, the proportion of ROS-positive cells, and the expression levels of SHC1 mRNA and protein and HDAC1 protein were higher than those in the Control group, and the above values in the H2 O2 + WTD groupwere lower than those in H 2O2 group (P< 0. 05). Conclusion WTD inhibits inflammatory and oxidative stress-induced injury in human UdSC, the mechanism may be achieved by modulating the Ahr / LOC101928120 / SHC1 signaling pathway.

Immunomodulatory Effect of Tetramethylpyrazine on MCP-1 in Allergic Rhinitis Rats #br#
CHENG Jie, WANG Wenguang
2025, 22(3):  259-265.  doi:10.3870/j.issn.1672-8009.2025.03.008
Abstract ( 15 )   PDF (5199KB) ( 4 )  
Related Articles | Metrics
Objective To investigate the immune regulatory effects of tetramethylpyrazine( TMP) on allergic rhinitis (AR) rats by regulating the MCP-1 / CCR2 signaling pathway. Methods Rats were randomly separated into 6 groups: Control group, Model group, TMP low-dose (TMP-L) group, TMP high-dose (TMP-H) group, TMP-H + rMCP-1 (recombinant MCP-1 protein) group, and loratadine group. AR rats were rated for their symptoms. The blood cell count method was applied to detect the number of various types of cells in the nasal lavage fluid of each group. Flow cytometry was applied to detect the levels of CD4 + , CD8 + , and CD4 + / CD8 + ratio in serum. ELISA was applied to detect serum levels of IL-10, IL-17, IL-6, interferon-γ ( IFN-γ), immunoglobulin (IgE), and allergenic mediator histamine (HIS). HE staining was applied to observe the pathological morphology of rat nasal mucosa. Western blotting was applied to detect the expression levels of aquaporins AQP1, AQP2, MCP-1, and CCR2 in nasal mucosa tissues. Results Compared with those in the Model group, the TMP-L, TMP-H and loratadine groups showed an obvious reduction in nasal mucosal tissue damage in rats, the symptom score of allergic rhinitis, numbers of eosinophils, neutrophils, lymphocytes, and macrophages in nasal lavage fluid, serum CD8 + level, IL-17, IL-6, IgE, HIS levels, and the expression levels of AQP1, AQP2, MCP-1, and CCR2 proteins in nasal mucosa tissues were decreased, and the serum CD4 + , CD4 + / CD8 + ratio, IL-10, and IFN-γ levels were increased (P< 0. 05). The values of above indexes in the TMPH and loratadine groups were at the same level (P > 0. 05). RMCP-1 could alleviate the improvement effect of TMP on AR rats (P < 0. 05). Conclusion TMP can reduce inflammation, alleviatenasal mucosal damage, and improve immune function in AR rats, which may be related to the inhibition of the MCP-1 / CCR2 signaling pathway.

Expression and Clinical Prognostic Value of MCUR1 in Triple-negative Breast Cancer #br#
FU Yuxing, PENG Qian, YANG Chaogang,
2025, 22(3):  266-270.  doi:10.3870/j.issn.1672-8009.2025.03.009
Abstract ( 15 )   PDF (1455KB) ( 5 )  
Related Articles | Metrics
Objective To analyze the expression of mitochondrial calcium uniporter regulator 1 (MCURl) in triple-negative breast cancer (TNBC) and its clinical significance. Methods  Immunohistochemistry was used to detect the expression of MCUR1 protein in TNBC tissues and corresponding adjacent tissues of 98 patients. The relationship between MCURl expression in tumor tissuesand patients’clinicopathological features and overall survival was further analyzed. Results  MCUR1was overexpressed in TNBC, and its expression level was significantly higher than that in the adjacent tissues (t = 34. 58, P < 0. 01). Clinicopathological analysis showed that high MCUR1 expression in TNBC was significantly correlated with tumor grade, T stage, and TNM stage ( P 0. 05,respectively). Further survival analysis showed that patients with high expression level of MCUR1had a lower overall survival rate than those with low expression level ( χ2 = 4. 591, P = 0. 032), which was an independent risk factor for poor prognosis of TNBC (HR = 2. 106, 95 % CI: 1. 013-5. 628, P = 0. 035). Conclusion  The expression of MCUR1 in TNBC tissues is up-regulated, andits high expression is associated with tumor progression and poor prognosis, which is a potential biomarker to evaluate the prognosis of TNBC patients.
Role of SIRT3 in Children with Acute Myeloid Leukemia #br#
REN Hua, HAO Qiang, GUO Li, ZHENG Suhua, WEI Lei
2025, 22(3):  271-276.  doi:10.3870/j.issn.1672-8009.2025.03.010
Abstract ( 17 )   PDF (1616KB) ( 5 )  
Related Articles | Metrics
Objective To explore the effect of silent information regulator 3 (SIRT3) in children with acute myeloid leukemia (AML). Methods The expression level of SIRT3 in bone marrow cells of AML children and peripheral blood leukocytes of healthy blood donor children was detected. SIRT3 expression was knocked down in AML cells. The cells’proliferation, apoptosis, oxidative stress indexes, and protein expression levels of the Wnt / β-catenin signaling pathway weredetected. Results Compared with those in the normal children group, the expression levels of SIRT3mRNA and protein were increased in the AML children group (P < 0. 05). After knocking down SIRT3expression in AML cells, the cells proliferation, the colony formation ability, the levels of glutathione (GSH) and superoxide dismutase (SOD), the total antioxidant capacity (T-AOC), the expression level of β-catenin, and the transcription activity of T cell factor / lymphocyte enhancer factor (TCF/ LEF) were decreased, while the apoptosis rate, the levels of reactive oxygen species (ROS), malondialdehyde (MDA) and phosphorylated glycogen synthase kinase 3β (p-GSK-3β) were increased (P<0. 05), the GSK-3β inhibitor TWS119 could partially reverse the above effects. Conclusion Knocking down SIRT3 can inhibit the growth of AML cells and increase oxidative stress level, which may berelated to the inhibition of Wnt / β-catenin signaling pathways.
Predictive Value of a Nomogram Prediction Model Based on Aspartate Aminotransferase to Platelet Ratio for Short-term Prognosis of Cervical Cancer Patients After Surgery #br#
CAI Qian, XIE Shuang, SONG Xiaoqing
2025, 22(3):  277-282.  doi:10.3870/j.issn.1672-8009.2025.03.011
Abstract ( 9 )   PDF (1450KB) ( 5 )  
Related Articles | Metrics
Objective To construct a predictive model for short-term postoperative prognosis ofcervical cancer patients based on the aspartate aminotransferase-to-platelet ratio index ( APRI).Methods A total of 112 patients diagnosed with cervical cancer and undergoing radical surgeryfrom January 2021 to August 2023 in Hubei Jianghan Oilfield General Hospital were included as the cervical cancer group, according to the inclusion and exclusion criteria. Additionally, 100 healthy women who underwent gynecological examinations during the same period were selected as the control group. During follow-up, cervical cancer patients were divided into a good prognosis group (81 cases) and a poor prognosis group (31 cases) based on their postoperative prognosis. General information was compared between the cervical cancer group and the control group, and clinical data from the cervical cancer group were collected to explore the risk factors affecting the short-term prognosis of cervical cancer patients after radical surgery. A nomogram model was constructed to predict the prognosis of cervical cancer patients after radical surgery. The ROC curve was used to analyze the predictivevalue of APRI for the short-term postoperative prognosis of cervical cancer patients. Results Compared with the control group, the cervical cancer group had higher APRI and high-risk HPV infection rates (P<0. 05). Significant differences were observed in tumor diameter, differentiation grade, FIGO stage, lymph node metastasis, AST, APRI, and HPV between the good prognosis group and thepoor prognosis group (all P<0. 05). Upon conducting Logistic regression analysis, it was determinedthat the following factors emerged as independent predictors of short-term unfavorable prognosis in cervical cancer patients following radical surgery: tumor diameter, degree of differentiation, FIGO stage, lymph node metastasis status, APRI values, and the presence of high-risk HPV infection (allP<0. 05). The calibration curve of the nomogram model indicated high applicability, with accurateand reliable predictions. The ROC curve results showed that APRI could serve as an effective diagnostic indicator for predicting short-term poor postoperative prognosis in cervical cancer patients (AUC =0. 743, P<0. 05). Conclusion APRI may have a reference value in predicting the short-term prognosis of cervical cancer patients undergoing radical surgery.

Effect of ω-3 Polyunsaturated Fatty Acids on Wound Healingin Rats with Chronic Refractory Wounds #br#
LI Xun, LI Desheng, CHEN Jie
2025, 22(3):  283-289.  doi:10.3870/j.issn.1672-8009.2025.03.012
Abstract ( 10 )   PDF (7866KB) ( 7 )  
Related Articles | Metrics
Objective To investigate the effect of ω-3 polyunsaturated fatty acids ( ω-3 PUFAs) on wound healing in rats with chronic refractory wounds. Methods SD rats were randomlyseparated into 6 groups: normal group, model group, ω-3 PUFAs low-, medium-, and high-dose groups, and Beifuxin group, with 10 rats in each group. Except for the normal group, the rats in the other groups were constructed with a chronic refractory wound model, and the wound healing ratio of the rats was measured after being treated with ω-3 PUFAs or Beifuxin. HE staining was used to determine the morphological structure of wound tissues. Immunohistochemical staining was used to detect the expression of Collagen Ⅱ and fibrin-binding protein (FN) and the expression of CD31 in the wound tissues to evaluate their microvascular density (MVD). ELISA detected the serum levels of pro-inflammatory factors TNF-α and IL-6. Western blotting was used to detect the expression levelsof pro-inflammatory factors and VEGF and TGF-β1 proteins in wound tissues. Results The levels ofTNF-α and IL-6, MVD, positive expressions of Collagen Ⅱ and FN, and the expression levels of TNF-α and IL-6, VEGF, TGF-β1 proteins in the model group were higher than those in the normalgroup (P < 0. 05). Compared with those in the model group, the levels of TNF-α and IL-6 and theprotein expression levels of TNF-α and IL-6 in the ω-3 PUFAs low-, medium-and high-dose groups and the Beifuxin group were decreased, the wound healing ratio, MVD, positive expressions ofCollagen Ⅱ and FN, the protein expression levels of VEGF and TGF-β1 were increased ( P < 0. 05). The changes in each index among ω-3 PUFAs groups were dose-dependent (P < 0. 05). No significant change was seen when compared those indexes between the Beifuxin group and the ω-3PUFAs high-dose group ( P > 0. 05). Conclusion  ω-3 PUFAs can inhibit wound inflammation,promote angiogenesis, and upregulate the expression of VEGF and TGF-β1, and promote the healing of chronic refractory wounds.

Effect of Phillyrin on Streptozotocin-induced Diabetic Mice and Its Mechanism #br#
MA Haiping, LI Hongling
2025, 22(3):  290-294.  doi:10.3870/j.issn.1672-8009.2025.03.013
Abstract ( 15 )   PDF (648KB) ( 5 )  
Related Articles | Metrics
Objective To explore the effect and mechanism of phillyrin on streptozotocin induced diabetic mouse model. Methods  A diabetic mouse Model was established and mice wererandomly divided into 6 groups: Control group, Model group, phillyrin low-dose group, phillyrin medium-dose group, phillyrin high-dose group, and metformin group. The blood glucose value was measured by a glucose meter. The glycosylated hemoglobin detector was used to detect glycosylated hemoglobin. The levels of serum urea nitrogen, creatinine, 24 h urine protein, and the levels of TC, TG, LDL-C, HDL-C, MDA, SOD and GSH-Px were detected by kits. The levels of TNF-α,IL-6, and IL-1β were detected by ELISA. Results Compared with those in the Control group, thelevels of serum urea nitrogen, creatinine and urine protein, the levels of TC, TG, LDL-C, thelevel of MDA content, and the levels of TNF-α, IL-6 and IL-1β were increased in the Modelgroup, while the level of HDL-C, and the levels of SOD and GSH-Px contents were decreased(P < 0. 05). Compared with those in the Model group, the levels of serum urea nitrogen, creatinineand urinary protein, the levels of TC, TG and LDL-C, the level of MDA content, and the levels of TNF-α, IL-6 and IL-1β were decreased in the phillyrin medium and high dose groups and metformin groups, while the level of HDL-C, and the levels of SOD and GSH-Px contents were increased.(P < 0. 05). Conclusion Phillyrin can protect streptozotocin-induced diabetic mice by regulation ofdyslipidemia, oxidative stress, and inflammation.
Research Progress of Ferroptosis of Vascular Endothelial Cells in Atherosclerosis #br#
ZHI Huimin, LI Yuanping, GAO Fen
2025, 22(3):  295-299.  doi:10.3870/j.issn.1672-8009.2025.03.014
Abstract ( 28 )   PDF (2761KB) ( 14 )  
Related Articles | Metrics
Atherosclerosis is the main pathological basis for cerebral infarction, coronary arteryatherosclerotic heart disease and other cardiovascular and cerebrovascular diseases. The abnormal death and functional disorder of vascular endothelial cells play an important role in it. Ferroptosis is a novel type of programmed cell death discovered in 2012, and there have been many studies showing that it plays an important role in the functional disorder and abnormal death of endothelial cells leading to AS. This review summarizes the mechanisms of ferroptosis and the possible mechanisms by which vascular endothelial cell ferroptosis leads to AS, and the drugs that may affect endothelial cell ferroptosis, providing a theoretical basis and research direction for the prevention and treatment of AS in clinical practice.
Mechanism of Pimozide in Acute Myeloid Leukemia #br# #br#
CHEN Chunxiao, LI Shuangyue,
2025, 22(3):  300-304.  doi:10.3870/j.issn.1672-8009.2025.03.015
Abstract ( 16 )   PDF (658KB) ( 14 )  
Related Articles | Metrics
Acute myeloid leukemia ( AML ) is a highly aggressive hematologicalmalignancy. Currently, chemotherapy and hematopoietic stem cell transplantation remain the primary treatment options for AML, yet approximately 30 % of patients experience relapse and have a poor prognosis. Pimozide, an antipsychotic drug, is primarily used in the treatment of schizophrenia, bipolar disorder, and other conditions. Several studies have now confirmed its antitumor effects. This article aims to review the mechanisms of pimozide in AML by literature analyzing, which provides insights into treatment strategies for AML.
News
 
More...
Download
More...
Links
More...
Visited
    Total visitors:
    Visitors of today:
    Now online: