关键词: circRNA_ 002178, CD133, 线粒体膜电位, 细胞凋亡, 卵巢癌

Abstract: Objective To study the effect of circRNA_ 002178 gene interference on malignant biological behavior of ovarian cancer cells. Methods Human ovarian cancer SKOV3 cells were divided into 4 groups: control group, short hairpin RNA negative control (shRNA-NC) group, circRNA_ 002178-shRNA 1 group and circRNA_ 002178-shRNA 2 group. The expression level of circRNA_ 002178 was detected by real-time fluorescence quantitative polymerase chain reaction (RTPCR). Flow cytometry was used to detect the cell apoptosis and mitochondrial membrane potential and to select the stem cell positive (CD44, CD133) cell lines. The stem cell spheroidization test was used to detect the sphere formation rate and the diameter of spheres. Transwell assay was used to detect the cell invasion. Western blotting was used to detect the expression levels of the proteins. Thetransplanted tumor model was constructed to observe the effect of circRNA_ 002178 gene interference on tumor formation. Results CircRNA_ 002178 interference significantly promoted the apoptosis ofSKOV3 cells, decreased the number of CD44 and CD133 positive cells, the rate of sphere formation, the diameter of spheres and the number of invasive cells, intensified the changes of mitochondrial membrane potential, and inhibited tumor growth and Akt / mTOR pathway activation in transplanted tumor rats. Conclusion CircRNA_ 002178 interference can inhibit the malignant biologicalbehavior of ovarian cancer cells. The mechanism may be related to the inhibitory effect on Akt / mTOR pathway.
 

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