lncRNA MIR100HG促进三阴乳腺癌MDA-MB-231细胞的上皮间质转化

doi:10.3870/j.issn.1672-8009.2025.06.010

医学分子生物学杂志 ›› 2025, Vol. 22 ›› Issue (6): 601-606.doi: 10.3870/j.issn.1672-8009.2025.06.010

lncRNA MIR100HG促进三阴乳腺癌MDA-MB-231细胞的上皮间质转化

李长春¹, 严文跃², 刘根祥¹, 周广军¹

盐城市第一人民医院¹普外科,²肿瘤科江苏省盐城市,224000

收稿日期:2024-11-28 出版日期:2025-11-30 发布日期:2025-12-25
通讯作者: 周广军(E-mail:zhouguangjun@ycsyy.com)
基金资助:
盐城市卫健委科技计划项目(No.YK2019003)

LncRNA MIR100HG Promotes Epithelial-mesenchymal Transition in Triple-negative Breast Cancer MDA-MB-231 Cells

LI Changchun¹, YAN Wenyue², LIU Genxiang¹, ZHOU Guangjun¹

¹Department of General Surgery,²Department of Oncology,Yancheng First People’s Hospital,Yancheng,Jiangsu,224000,China

Received:2024-11-28 Online:2025-11-30 Published:2025-12-25
Contact: ZHOU Guangjun(E-mail:zhouguangjun@ycsyy.com)
Supported by:
Yancheng Municipal Health Commission Science and Technology Plan Project(No.YK2019003)

摘要/Abstract

摘要： 目的探究长链非编码RNA(long non-coding RNA,lncRNA)MIR100HG对三阴乳腺癌MDA-MB-231细胞上皮间质转化的影响。方法在三阴性乳腺癌MDA-MB-231细胞中分别进行MIR100HG的过表达和抑制处理后,采用MTS法检测细胞增殖能力,采用流式细胞术分析细胞凋亡率,采用Transwell检测细胞迁移和侵袭能力;采用蛋白质印迹分析MIR100HG对上皮-间质转化相关标志物的调控作用。结果 MIR100HG过表达能够促进MDA-MB-231细胞增殖、迁移和侵袭,减少细胞凋亡,并能下调上皮标志物和上调间质标志物的表达;抑制MIR100HG后以上结果相反。结论 lncRNA MIR100HG过表达能够促进三阴乳腺癌MDA-MB-231细胞增殖、迁移、侵袭,减少MDA-MB-231细胞凋亡,诱导MDA-MB-231细胞的上皮-间质转化过程。

关键词: 长链非编码RNA, MIR100HG, 上皮-间质转化, MDA-MB-231

Abstract: Objective To investigate the effect of long non-coding RNA(lncRNA)MIR100HG on epithelial-mesenchymal transition(EMT)of triple-negative breast cancer MDA-MB-231 cells. Methods MIR100HG was overexpressed or inhibited in the MDA-MB-231 cells,cell proliferation was measured by MTS assay,cell apoptosis rate was analyzed by flow cytometry,and cell migration and invasion were determined by Transwell assay.Western blotting was used to detect the regulatory effect of MIR100HG on epithelial-mesenchymal transition related markers. Results Overexpression of MIR100HG promoted the proliferation,migration and invasion of MDA-MB-231 cells,reduced cell apoptosis,downregulated the expression of epithelial markers and upregulated the expression of mesenchymal markers; Inhibition of MIR100HG produced opposite results. Conclusion Overexpression of LncRNA MIR100HG can promote the proliferation,migration and invasion of MDA-MB-231 cells,reduce the apoptosis of MDA-MB-231 cells,and induce the epithelial-mesenchymal transition of MDA-MB-231 cells.

Key words: long non-coding RNA, MIR100HG, epithelial-mesenchymal transition, MDA-MB-231

中图分类号:

R363

李长春, 严文跃, 刘根祥, 周广军. lncRNA MIR100HG促进三阴乳腺癌MDA-MB-231细胞的上皮间质转化[J]. 医学分子生物学杂志, 2025, 22(6): 601-606.

LI Changchun, YAN Wenyue, LIU Genxiang, ZHOU Guangjun. LncRNA MIR100HG Promotes Epithelial-mesenchymal Transition in Triple-negative Breast Cancer MDA-MB-231 Cells[J]. Journal of Medical Molecular Biology, 2025, 22(6): 601-606.

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lncRNA MIR100HG促进三阴乳腺癌MDA-MB-231细胞的上皮间质转化

LncRNA MIR100HG Promotes Epithelial-mesenchymal Transition in Triple-negative Breast Cancer MDA-MB-231 Cells

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