lncRNA CRNDE通过ERK1/2通路影响溃疡性结肠炎小鼠体内巨噬细胞焦亡和M1型极化

doi:10.3870/j.issn.1672-8009.2026.01.005

医学分子生物学杂志 ›› 2026, Vol. 23 ›› Issue (1): 36-42.doi: 10.3870/j.issn.1672-8009.2026.01.005

lncRNA CRNDE通过ERK1/2通路影响溃疡性结肠炎小鼠体内巨噬细胞焦亡和M1型极化

翡罗热·地里夏提¹, 祖丽胡玛尔·阿力木江², 杜进璇¹

新疆医科大学第六附属医院¹消化科,²全科医学科乌鲁木齐市,830000

收稿日期:2025-01-16 发布日期:2026-01-29
通讯作者: 杜进璇(E-mail:djx1124@163.com)
基金资助:
新疆维吾尔自治区自然科学基金(No.2023D01C423)

Effect of lncRNA CRNDE on Macrophage Pyroptosis and M1-type Polarization in Ulcerative Colitis Mice via ERK1/2 Pathway

Feiluore Dilixiati¹, Zulihumaer Alimujiang², DU Jinxuan¹

¹Department of Gastroenterology,²Department of General Medicine,Sixth Affiliated Hospital of Xinjiang Medical University,Urumqi,830000,China

Received:2025-01-16 Published:2026-01-29
Contact: DU Jinxuan(E-mail:djx1124@163.com)
Supported by:
Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2023D01C423)

摘要/Abstract

摘要： 目的探讨长链非编码RNA(lncRNA)CRNDE通过细胞外信号调节激酶1/2(ERK1/2)通路对溃疡性结肠炎(ulcerative colitis,UC)小鼠巨噬细胞焦亡和M1型极化的影响。方法构建UC小鼠模型,检测结肠长度,进行疾病活动度指数评分以验证模型;另将RAW264.7细胞分为Ctrl组、pcDNA-null组、pcDNA-CRNDE组、pcDNA-CRNDE+LY3214996组。qRT-PCR、蛋白质印迹检测lncRNA CRNDE及cleaved-Caspase-1、cleaved-GSDMD、ERK1/2、磷酸化ERK1/2(p-ERK1/2)的表达水平,流式细胞术检测F4/80⁺CD86⁺巨噬细胞比例。结果模型组疾病活动度指数评分升高、结肠缩短,lncRNA CRNDE及cleaved-Caspase-1、cleaved-GSDMD、p-ERK1/2表达上调,F4/80⁺CD86⁺巨噬细胞比例增加(P_均<0.05);在RAW264.7细胞中,pcDNA-CRNDE组的lncRNA CRNDE及cleaved-Caspase-1、cleaved-GSDMD、p-ERK1/2表达较pcDNA-null组上调,加入LY3214996后lncRNA CRNDE及cleaved-Caspase-1、cleaved-GSDMD、p-ERK1/2表达下调(P_均<0.05)。结论 lncRNA CRNDE在UC小鼠中通过激活ERK1/2途径,促进巨噬细胞焦亡和M1型极化,是治疗UC的潜在靶点。

关键词: 溃疡性结肠炎, lncRNA CRNDE, 巨噬细胞, 焦亡, M1型极化

Abstract: Objective To investigate the effect of long non-coding RNA(lncRNA)CRNDE on macrophage pyroptosis and M1 polarization in ulcerative colitis(UC)mice and the potential mechanism. Methods The UC mouse model was established,and colon length,disease activity index(DAI)were measured to validate the model.RAW264.7 cells were divided into 4 groups:Ctrl group,pcDNA-null group,pcDNA-CRNDE group,and pcDNA-CRNDE+LY3214996 group.The expression levels of lncRNA CRNDE,cleaved-Caspase-1,cleaved-GSDMD,ERK1/2,and phosphorylated ERK1/2(p-ERK1/2)were detected by qRT-PCR or Western blotting.The proportion of F4/80⁺CD86⁺macrophages was measured by flow cytometry. Results In the model group,the DAI score was increased,the colon length was shortened,and the expression levels of lncRNA CRNDE,cleaved-Caspase-1,cleaved-GSDMD,and p-ERK1/2 were upregulated,and the proportion of F4/80⁺CD86⁺ macrophages was increased(all P<0.05).In RAW264.7 cells,lncRNA CRNDE and cleaved-Caspase-1、cleaved-GSDMD、p-ERK1/2 expression in the pcDNA-CRNDE group was upregulated when compared with that in the pcDNA-null group,and that was downregulated after LY3214996 treatment(all P<0.05). Conclusion lncRNA CRNDE promotes macrophage pyroptosis and M1 polarization in UC mice by activating the ERK1/2 pathway,suggesting it may be a potential therapeutic target for UC.

Key words: ulcerative colitis, lncRNA CRNDE, macrophage, pyroptosis, M1 polarization

中图分类号:

R342.3

翡罗热·地里夏提, 祖丽胡玛尔·阿力木江, 杜进璇. lncRNA CRNDE通过ERK1/2通路影响溃疡性结肠炎小鼠体内巨噬细胞焦亡和M1型极化[J]. 医学分子生物学杂志, 2026, 23(1): 36-42.

Feiluore Dilixiati, Zulihumaer Alimujiang, DU Jinxuan. Effect of lncRNA CRNDE on Macrophage Pyroptosis and M1-type Polarization in Ulcerative Colitis Mice via ERK1/2 Pathway[J]. Journal of Medical Molecular Biology, 2026, 23(1): 36-42.

参考文献

[1] SEGAL J P,LEBLANC J F,HART A L.Ulcerative colitis:an update[J].Clin Med,2021,21(2):135-139.
[2] PORTER R J,KALLA R,HO G T.Ulcerative colitis:recent advances in the understanding of disease pathogenesis[J].F1000 Res,2020,9:294.
[3] FENTON C G,RAY M K,MENG W,et al.Methylation-regulated long non-coding RNA expression in ulcerative colitis[J].Int J Mol Sci,2023,24(13):10500.
[4] TIAN C,GAO J,YANG L,et al.Non-coding RNA regulation of macrophage function in asthma[J].Cell Signal,2023,112:110926.
[5] CHINI A,GUHA P,MALLADI V S,et al.Novel long non-coding RNAs associated with inflammation and macrophage activation in human[J].Sci Rep,2023,13(1):4036.
[6] DING N,LUO H,PENG T,et al.Bioinformatics analysis on differentially expressed genes between colorectal adenoma and colorectal adenocarcinoma[J].Scot Med J,2022,67(4):178-188.
[7] TANG D,ZHAO L,MU R,et al.LncRNA colorectal neoplasia differentially expressed promotes glycolysis of liver cancer cells by regulating hypoxia-inducible factor 1α[J].Chin J Physiol,2022,65(6):311-318.
[8] ZHANG Q,ZHU D,LI Q.LncRNA CRNDE exacerbates neuropathic pain in chronic constriction injury-induced(CCI)rats through regulating miR-146a-5p/WNT5A pathway[J].Bioengineered,2021,12(1):7348-7359.
[9] YANG F,LI X F,CHENG L N,et al.Long non-coding RNA CRNDE promotes cell apoptosis by suppressing miR-495 in inflammatory bowel disease[J].Exp Cell Res,2019,382(2):111484.
[10] KAZEMIFARD N,FARMANI M,BARADARAN GHAVAMI S,et al.A prediction of the CRNDE role by modulating NF-κB pathway in inflammatory bowel disease(IBD)[J].Biochem Biophys Rep,2024,38:101731.
[11] YIN L,YAN J,CHEN W.Mechanism of lncRNA-H19 inintestinal injury of mice with ulcerative colitis[J].Int Arch Allergy Immunol,2022,183(9):985-996.
[12] CAO L,TAN Q,ZHU R,et al.LncRNA MIR4435-2 HG suppression regulates macrophage M1/M2 polarization and reduces intestinal inflammation in mice with ulcerative colitis[J].Cytokine,2023,170:156338.
[13] YUAN Y,HE Y,WASTI B,et al.lncRNA CRNDE affects Th17/IL-17 A and inhibits epithelial-mesenchymal transition in lung epithelial cells reducing asthma signs[J].Oxid Med Cell Longev,2023,2023:2092184.
[14] HEYDARI R,FAYAZZADEH S,SHAHROKH S,et al.Plasma extracellular vesicle LncRNA H19 as a potential diagnostic biomarker for inflammatory bowel diseases[J].Inflamm Bowel Dis,2024,30(5):795-807.
[15] DAI Y,LU Q,LI P,et al.Xianglian Pill attenuates ulcerative colitis through TLR4/MyD88/NF-κB signaling pathway[J].J Ethnopharmacol,2023,300:115690.
[16] ZHUANG H,LV Q,ZHONG C,et al.Tilirosideameliorates ulcerative colitis by restoring the M1/M2 macrophage balance via the HIF-1α/glycolysis pathway[J].Front Immunol,2021,12:649463.
[17] LIANG L,LIU L,ZHOU W,et al.Gut microbiota-derived butyrate regulates gut mucus barrier repair by activating the macrophage/WNT/ERK signaling pathway[J].Clin Sci,2022,136(4):291-307.

lncRNA CRNDE通过ERK1/2通路影响溃疡性结肠炎小鼠体内巨噬细胞焦亡和M1型极化

Effect of lncRNA CRNDE on Macrophage Pyroptosis and M1-type Polarization in Ulcerative Colitis Mice via ERK1/2 Pathway

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