海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶治疗幽门螺旋杆菌感染小鼠模型的抗菌活性研究

doi:10.3870/j.issn.1672-8009.2026.01.004

医学分子生物学杂志 ›› 2026, Vol. 23 ›› Issue (1): 27-35.doi: 10.3870/j.issn.1672-8009.2026.01.004

海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶治疗幽门螺旋杆菌感染小鼠模型的抗菌活性研究

卡斯木·卡哈尔¹, 阿依努尔·吐鲁洪², 阿迪力·阿不都热合曼³

¹新疆医科大学第一附属医院药学部制剂科乌鲁木齐市,830054
²新疆医科大学第一附属医院昌吉分院药学部药品供应调剂科乌鲁木齐市,831100
³新疆医科大学药学院乌鲁木齐市,830017

收稿日期:2025-04-24 发布日期:2026-01-29
通讯作者: 阿迪力·阿不都热合曼(E-mail:39674971@qq.com)
基金资助:
新疆维吾尔族自治区自然科学基金(No.2023D01C329)

Antibacterial Activity of Alginate-Silver-S-Nitrosoglutathione Nanoparticle Gel in Treatment of Helicobacter Pylori Infected Mouse Model

Kasimu Kahaer¹, Ayinuer Tuluhong², Adili Abudureheman³

¹Department of Pharmaceutical Preparations,First Affiliated Hospital of Xinjiang Medical University,Urumqi,830054,China
²Department of Pharmaceutical Supply and Adjustment,Changji Branch of the First Affiliated Hospital of Xinjiang Medical University,Urumqi,831100,China
³School of Pharmacy,Xinjiang Medical University,Urumqi,830017,China

Received:2025-04-24 Published:2026-01-29
Contact: Adili Abudureheman(E-mail:39674971@qq.com)
Supported by:
Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2023D01C329)

摘要/Abstract

摘要： 目的探讨海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒(nanoparticles,NPS)凝胶治疗幽门螺旋杆菌(Helicobacter pylori,H.pylori)感染小鼠模型的抗菌活性。方法制备海藻酸盐纳米颗粒(alginate NPs)凝胶,海藻酸盐-银纳米颗粒(alginate-Ag NPs)凝胶和海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒(alginate-Ag-GSNO NPs)凝胶。扫描电镜成像Ag-GSNO纳米颗粒凝胶表面特征。建立H.pylori感染小鼠模型。40只雄性C57BL/6小鼠,随机分为4组,每组10只:vehicle组,model组,alginate-Ag NPs凝胶组,alginate-Ag-GSNO NPs凝胶组。血琼脂培养并进行小鼠胃上皮组织H.pylori感染的菌落形成单位(colony forming units,CFU)计数。HE染色和组织病理学分析小鼠胃上皮组织炎症和损伤。qPCR法测定小鼠胃上皮组织H.pylori感染阳性标志物ureB基因的表达水平。ELISA法测定炎症因子TNF-α、IL-1β、IL-6水平。蛋白质印迹法测定NLRP3炎症小体的表达水平。结果海藻酸盐-银纳米颗粒具有球形形貌,粒径范围为100~200 nm。GSNO颗粒呈球形,略有凹陷,尺寸为μm级。与alginate-Ag NPs凝胶组比较,Alginate-Ag-GSNO NPs凝胶组显著降低了小鼠胃上皮组织感染的H.pylori的CFU(P<0.05);小鼠胃上皮组织HE染色评分降低(P<0.05),胃上皮细胞排列较为整齐,存在一定量多形核细胞浸润、淋巴样聚集体和H.pylori感染阳性细胞;小鼠胃上皮组织H.pylori感染阳性标志物ureB基因的表达水平降低(P<0.05);炎症因子TNF-α、IL-1β、IL-6的水平降低(P<0.05);NLRP3炎症小体的相对表达水平降低(P<0.05)。结论相比较于海藻酸盐-银纳米颗粒凝胶,海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶能更有效地抑制H.pylori 感染小鼠模型H.pylori的定植和对胃上皮组织的损伤。

关键词: 幽门螺旋杆菌, 海藻酸盐-银纳米颗粒凝胶, 海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶, 胃上皮组织, 抗菌活性

Abstract: Objective To investigate the antibacterial activity of alginate-silver-S-nitrosoglutathione nanoparticle(NP) gel in the treatment of Helicobacter pylori(H.pylori)infected mouse model. Methods Alginate NPs gel,alginate-Ag NPs gel and alginate-Ag-GSNO NPs gel were prepared.The surface characteristics of Ag-GSNO nanoparticle gel were imaged by scanning electron microscopy.A mouse model of H.pylori infection was established.A total of 40 male C57BL/6 mice were randomly divided into 4 groups with 10 mice each:Vehicle group,Model group,Alginate-Ag NPs gel group,Alginate-AG-GSNO NPs gel group.The colony forming unit(CFU)counting of H.pylori infection in mice gastric epithelium was performed with blood agar culture.HE staining and histopathology were used to analyze the inflammation and injury of gastric epithelium in mice.The expression levels of ureB gene,which is a positive marker of H.pylori infection,was determined by qPCR.The levels of inflammatory cytokines TNF-α,IL-1β and IL-6 were determined by ELISA.The expression levels of NLRP3 inflammasome was determined by Westren blotting. Results Alginate-Ag nanoparticles had a spherical morphology,with a particle size range of 100-200 nm.Spray dried GSNO particles were spherical with slight depressions,and their size was at the μm level.Compared with those in the Alginate-Ag NPs gel group,the CFU of H.pylori infected mice gastric epithelial tissues was decreased(P<0.05),and the HE staining score was reduced(P<0.05)in the Alginate-AG-GSNO NPs gel group.The Gastric epithelial cells in the Alginate-AG-GSNO NPs gel group were arranged orderly in the Alginate-AG-GSNO NPs gel group,and there were a reduced number of polymorphonuclear infiltration,lymphoid aggregation and H.pylori infection positive cells,indicating the improvement of inflammation and injury of gastric epithelial tissues.The expression levels of ureB gene,the relative expression levels of NLRP3 inflammasome,and the levels of inflammatory cytokines TNF-α,IL-1β and IL-6 were all decreased(all P<0.05). Conclusion Compared with alginate-Ag NPs gel,alginate-AG-GSNO NPs gel could more effectively inhibit the colonization of H.pylori in infected mouse model and improve gastric epithelium damage.

Key words: Helicobacter pylori, alginate-Ag nanoparticles gel, alginate-Ag-GSNO nanoparticles gel, gastric epithelial tissue, antibacterial activity

中图分类号:

卡斯木·卡哈尔, 阿依努尔·吐鲁洪, 阿迪力·阿不都热合曼. 海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶治疗幽门螺旋杆菌感染小鼠模型的抗菌活性研究[J]. 医学分子生物学杂志, 2026, 23(1): 27-35.

Kasimu Kahaer, Ayinuer Tuluhong, Adili Abudureheman. Antibacterial Activity of Alginate-Silver-S-Nitrosoglutathione Nanoparticle Gel in Treatment of Helicobacter Pylori Infected Mouse Model[J]. Journal of Medical Molecular Biology, 2026, 23(1): 27-35.

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海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶治疗幽门螺旋杆菌感染小鼠模型的抗菌活性研究

Antibacterial Activity of Alginate-Silver-S-Nitrosoglutathione Nanoparticle Gel in Treatment of Helicobacter Pylori Infected Mouse Model

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