医学分子生物学杂志 ›› 2025, Vol. 22 ›› Issue (3): 217-222.doi: 10.3870/j.issn.1672-8009.2025.03.002

• 论著 • 上一篇    下一篇

奥拉帕尼对卵巢癌 ES2 细胞生存、 上皮细胞间质转换及肿瘤干细胞样特性的调节作用 #br#

  

  1. 陆军军医大学士官学校附属医院1预防保健科,2妇产科,4门诊部 石家庄市, 050041 3陆军军医大学士官学校战救医疗系 石家庄市, 050041
  • 出版日期:2025-05-31 发布日期:2025-06-12
  • 基金资助:
    河北省卫生健康委科研基金项目 (No. 20191730), 河北省 2022 年度医学科学研究课题 (No. 20221905)

Effect of Olaparib on Survival, Epithelial-mesenchymal Transition, and Tumor Stem Cell-like Characteristics in Ovarian Cancer ES2 Cells #br#

  1. 1Department of Preventive Health Care,2Department of Obstetrics and Gynecology,4 Department of Outpatient, the Affiliated Hospital of Army Medical University, Shijiazhuang, 050041, China  3Department of Combat Rescue Medicine, Army Medical University, Shijiazhuang, 050041, China
  • Online:2025-05-31 Published:2025-06-12

PDF

8

摘要： 目的 探究奥拉帕尼对卵巢癌 ES2 细胞生存、 上皮细胞间质转换 ( epithelial-mesenchymal transition, EMT) 及肿瘤干细胞样特性的调节作用。 方法 体外培养卵巢癌 ES2 细胞, 将其分为对照组、 奥拉帕尼低、 中、 高剂量组和阿霉素 0. 5 μmol / L 组。 分别使用 5-乙炔基-2′-脱氧尿苷 (EDU) 染色、 流式细胞术、Transwell 小室及划痕实验检测细胞增殖、 凋亡、 侵袭及迁移能力, 光镜下观察细胞上皮间质转化, 成瘤成球实验观察肿瘤干细胞样特性, 蛋白质印迹法检测相关蛋白表达。 结果 与对照组比较, 奥拉帕尼低、 中、高剂量组和阿霉素 0. 5 μmol / L 组 ES2 细胞凋亡率、 E-钙黏素 ( E-cadherin) 表达显著升高 ( P< 0. 05), 奥拉帕尼中、 高剂量组和阿霉素 0. 5 μmol / L 组 EDU 染色阳性细胞数比值、 单位面积细胞侵袭数比例、 细胞伤口愈合率、 细胞成球数目、 成球直径、 核抗原 Ki67、 存活素 ( Survivin)、 血管内皮生长因子 ( VEGF)、N-钙黏素 (N-cadherin)、 分化簇 44 ( CD44) 和乙醛脱氢酶 1 ( ALDH1) 表达显著降低 ( P< 0. 05)。 结论 30 mg / L及以上剂量奥拉帕尼可以显著抑制卵巢癌 ES2 细胞增殖、 侵袭及迁移, 促进其凋亡, 可能与抑制增殖蛋白 Ki67 并调节 EMT 过程有关。

关键词:

卵巢癌, 聚腺苷二磷酸核糖聚合酶, 奥拉帕尼, 细胞凋亡, 细胞迁移

Abstract: Objective To explore the effect of olaparib on the survival, epithelial-mesenchymal transition ( EMT ) and tumor stem cell-like characteristics of ovarian cancer ES2cells. Methods Ovarian cancer ES2 cells were culturedin vitro, and they were divided into 5groups: control group, low-dose olaparib group, middle-dose olaparib group, high-dose olaparib group and doxorubicin 0. 5 μmol / L group. Cells proliferation, apoptosis, invasion and migration were detected by 5-ethynyl-2′-deoxyuridine ( EDU) staining, flow cytometry, Transwell assay, and wound healing assay, respectively. The epithelial-mesenchymal transition ( EMT) of cells was observed under light microscope, tumor stem cell-like characteristics were observed by tumor spheroid formation assay, and protein expression level was detected by Western blotting. Results Compared with those in the control group, the apoptosis rate of ES2 cells and the expression level of Ecadherin were significantly increased in the low-, middle-, high-dose olaparib group and doxorubicin 0. 5 μmol / L group ( P < 0. 05), the ratio of EDU staining positive cells, number of invasioncells per unit area, cell wound-healing rate, number of tumor spheroid, spheroid diameter, and the expression levels of nuclear antigen Ki67, Survivin, vascular endothelial growth factor(VEGF), N-cadherin, cluster of differentiation 44 (CD44) and aldehyde dehydrogenase 1 (ALDH1) were significantly decreased in the middle-and high-dose olaparib group and doxorubicin 0. 5μmol / L group (P< 0. 05). Conclusion Olaparib of over 30 mg / L can significantly inhibit proliferation, invasion, and migration of ovarian cancer ES2 cells, and promote their apoptosis, which may be through inhibition of Ki67 and regulation of EMT.

Key words:

ovarian cancer, poly (ADP-ribose) polymerase, olaparib, apoptosis, cell migration

中图分类号: 

版权所有 © 《医学分子生物学杂志》编辑部
地址：湖北省武汉市航空路13号 邮编：430030
　电话：027-83692515　传真：027-83692927　E-mail：jmmb@hust.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发