翠云草总黄酮通过调控 miR-3662 表达抑制喉鳞癌 TU177 细胞增殖、迁移和侵袭的机制研究

doi:10.3870/j.issn.1672-8009.2023.03.013

摘要/Abstract

摘要： 目的探讨翠云草总黄酮 ( total flavonoids from Selaginella uncinata, TFS) 对喉鳞癌 TU177 细胞增殖、迁移和侵袭的影响及可能机制。方法体外培养 TU177 细胞, 分为对照组、不同剂量 (5、 15、 25 μg / mL) TFS 组, CCK-8 法和克隆形成实验检测细胞增殖, 划痕实验检测细胞迁移, Transwell 检测细胞侵袭, Western 印迹检测细胞中上皮型钙粘蛋白 (E-Cadherin)、神经型钙粘蛋白 (N-Cadherin) 表达, RT-qPCR 法检测 miR-3662 表达。收集 35 例喉鳞癌患者的癌组织及癌旁组织, RT-qPCR 法检测组织中 miR-3662 表达。另取 TU177 细胞, 分为 miR-3662 组、 miR-NC 组、 25 μg / mL TFS + anti-miR-3662 组和 25 μg / mL TFS + anti-miR-NC, CCK-8 法和克隆形成实验检测细胞增殖, 划痕实验检测细胞迁移, Transwell 检测细胞侵袭, Western 印迹检测细胞中 E-Cadherin 和 N-Cadherin 蛋白表达。结果与对照组比较, 不同剂量 TFS 组 TU177 细胞抑制率升高 (P< 0. 05), 克隆形成数、划痕愈合率和侵袭数及细胞中 N-Cadherin 蛋白表达降低 (P< 0. 05), E-Cadherin 蛋白和 miR-3662 表达升高 (P< 0. 05), 且呈剂量依赖性。喉鳞癌组织中 miR-3662 的表达较癌旁组织显著降低 (P< 0. 05)。与 miR-NC 组比较, miR-3662 组喉鳞癌 TU177 细胞抑制率和细胞中 E-Cadherin 蛋白表达升高 (P< 0. 05), 克隆形成数、迁移和侵袭数及细胞中 N-Cadherin 蛋白表达均降低 (P< 0. 05)。与 25 μg / mL TFS + anti-miR-NC 组比较, 25 μg / mL TFS + anti-miR-3662 组 TU177 细胞抑制率和细胞中 E-Cadherin 蛋白表达降低 (P< 0. 05), 克隆形成数、迁移和侵袭数及细胞中 N-Cadherin 蛋白表达均升高 (P< 0. 05)。结论翠云草总黄酮可抑制喉鳞癌 TU177 细胞增殖、迁移和侵袭, 其作用机制可能与上调细胞中 miR-3662 表达有关。

关键词: 翠云草总黄酮, 喉鳞癌, miR-3662, 细胞增殖, 迁移, 侵袭

Abstract: Objective To investigate the effect of total flavonoids from Selaginella uncinata (TFS) on the proliferation, migration and invasion of laryngeal squamous cell carcinoma TU177 cells and its possible mechanism. Methods TU177 cells were cultured in vitro and were divided into control group, TFS-5 μg / mL, - 15 μg / mL, - 25 μg / mL groups, miR-3662 group, miR-NC group, 25 μg / mL TFS + anti-miR-3662 group and 25 μg / mL TFS + anti-miR-NC group. CCK-8 method and colony formation assay were used to detect cell proliferation. Scratch assay was used to detect cell migration. Transwell assay was used to detect cell invasion. Western blotting was used to detect the protein expression levels of epithelial cadherin (E-Cadherin) and neural cadherin (N-Cadherin) in cells, and RT-qPCR method was used to detect the expression level of miR-3662. Results Compared with the control group, the cell inhibition rate of TFS groups was increased (P< 0. 05), but the number of colonies, the wound healing rate and the number of invasions, and the expression level of N-Cadherin protein were decreased (P < 0. 05), while the expression levels of E-Cadherin protein and miR-3662 were increased (P < 0. 05), all in a dose pendent manner. The expression level of miR-3662 in the laryngeal squamous cell carcinoma tissues was significantly lower than that in the adjacent tissues (P < 0. 05). Compared with the miR-NC group, the cell inhibition rate and the expression level of E-Cadherin protein in the miR-3662 group were increased (P< 0. 05), but the number of colonies, the wound healing rate and the number of invasions, and the expression level of N-Cadherin protein were decreased (P<0. 05). Compared with the 25 μg / mL TFS + anti-miR-NC group, the cell inhibition rate and the expression level of E-Cadherin protein in the 25 μg / mL TFS + anti-miR-3662 group were decreased (P<0. 05), but the number of colonies, the wound healing rate and the number of invasions, and the expression level of N-Cadherin protein were increased (P<0. 05). Conclusion Total flavonoids from Selaginella uncinata could inhibit the proliferation, migration and invasion of laryngeal squamous cell carcinoma TU177 cells, and its mechanism may be related to the up-regulation of miR-3662 expression in the cells.

Key words: total flavonoids from Selaginella uncinata, laryngeal squamous cell carcinoma, miR-3662, cell proliferation, migration, invasion

中图分类号:

李新华, 李军, 裴卓, 栾红娟. 翠云草总黄酮通过调控 miR-3662 表达抑制喉鳞癌 TU177 细胞增殖、迁移和侵袭的机制研究[J]. 医学分子生物学杂志, 2023, 20(3): 260-266.

LI Xinhua, LI Jun, PEI Zhuo, LUAN Hongjuan. Mechanism of Total Flavonoids from Selaginella uncinata Inhibiting the Proliferation, Migration and Invasion of Laryngeal Squamous Cell Carcinoma TU177 Cells by Regulation of miR-3662[J]. Journal of Medical Molecular Biology, 2023, 20(3): 260-266.

[1]	田莉, 赵济华, 徐晋珩, 胡月明, 崔海军, 曹渤海. LncRNA OSTN-AS1 下调 miR-4461 表达促进前列腺癌 LNCaP 细胞增殖、迁移和侵袭[J]. 医学分子生物学杂志, 2023, 20(3): 215-220.
[2]	吴红琴 , 王曦 , 谭云清 , 杨清 , 张静 , 臧兴双. RABL6 高表达促进口腔鳞癌细胞的增殖、迁移和侵袭[J]. 医学分子生物学杂志, 2023, 20(3): 254-259.
[3]	邓亮亮, 张先梅, 黄婧, 朱彬, 周奇. 敲低 CXCL8 抑制口腔鳞癌的细胞增殖和转移 [J]. 医学分子生物学杂志, 2023, 20(2): 110-116.
[4]	刘慧 , 王佳 , 张虹丽. PCA3 调控 miR-185 对霍奇金淋巴瘤细胞 L428 增殖和凋亡的影响[J]. 医学分子生物学杂志, 2023, 20(2): 141-147.
[5]	赵伟军, 苗艳凌, 斯日古楞, 李玉石. 蜘蛛香总黄酮调控 miR-4429 影响胃癌 AGS 细胞的增殖、迁移及侵袭[J]. 医学分子生物学杂志, 2023, 20(2): 173-178.
[6]	黄琦, 翟海程. lncRNA CERS6-AS1 通过调控 miR-138-2-3p 抑制人胶质瘤细胞系增殖 [J]. 医学分子生物学杂志, 2023, 20(1): 20-25.
[7]	鲁慧玲, 闫飞艳 , 常丽花. lncRNA PSMA3-AS1 靶向 miR-3619-5p 调控宫颈癌 SiHa 细胞增殖、迁移及侵袭的分子机制研究[J]. 医学分子生物学杂志, 2023, 20(1): 26-33.
[8]	郁昊 , 万炳年 , 刘艳萍. CHRDL1 调节结直肠癌增殖、迁移和侵袭[J]. 医学分子生物学杂志, 2023, 20(1): 63-69.
[9]	叶玉锦, 黄丽珊, 黄巧如, 吴明秀. lncRNA SNHG17 靶向 miR-525-5p 对人绒毛膜滋养层细胞增殖、迁移和侵袭的影响 [J]. 医学分子生物学杂志, 2022, 19(6): 452-456.
[10]	刘昕, 李天客 , 杜宁 , 路月亭 , 刘学聪. CD168 对口腔鳞状细胞癌增殖、侵袭的作用机制研究 [J]. 医学分子生物学杂志, 2022, 19(6): 457-463.
[11]	蒋君霞 , 杨国荣 , 谷涉群 , 喻珣. circ_ 0061140 靶向 miR-6838-5p 促进非小细胞肺癌细胞的增殖、阻滞细胞周期、诱导细胞凋亡[J]. 医学分子生物学杂志, 2022, 19(6): 464-471.
[12]	杨三虎, 李岩. 沉默 NEK2 通过调节 Wnt 信号通路抑制非小细胞肺癌的细胞增殖[J]. 医学分子生物学杂志, 2022, 19(6): 472-477.
[13]	郭秀莲 , 邢海 , 王巍. miR-766-5p 过表达介导 MMP-7 对胶质瘤细胞增殖、侵袭与凋亡的影响及机制 [J]. 医学分子生物学杂志, 2022, 19(5): 360-365.
[14]	李明辉, 王裕, 白晓刚. circUBE2D2 靶向 miR-376a-3p 调控结直肠癌 SW620 细胞的增殖、迁移及侵袭 [J]. 医学分子生物学杂志, 2022, 19(4): 326-331.
[15]	张玲艳 , 王海红 , 刘晶晶. miR-502-3p 通过靶向结合 CBL 抑制卵巢癌增殖并诱导凋亡[J]. 医学分子生物学杂志, 2022, 19(3): 181-186.

翠云草总黄酮通过调控 miR-3662 表达抑制喉鳞癌 TU177 细胞增殖、迁移和侵袭的机制研究

Mechanism of Total Flavonoids from Selaginella uncinata Inhibiting the Proliferation, Migration and Invasion of Laryngeal Squamous Cell Carcinoma TU177 Cells by Regulation of miR-3662

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价