关键词: 子痫前期, 人绒毛膜滋养层细胞, lncRNA SNHG17, miR-525-5p, 细胞增殖, 迁移, 侵袭

Abstract: Objective To investigate the effect of lncRNA SNHG17 on the growth and metastasis of human chorionic trophoblast cells and its possible mechanism. Methods The expression levels of lncRNA SNHG17 and miR-525-5p in normal placental tissues and preeclampsia placental tissues were detected by qRT-PCR. Human chorionic trophoblast cells HTR-8 / SVneo were culturedin vitroand were transfected with the si-SNHG17, anti-miR-525-5p and their negative controls, respectively. The plate colony formation assay, scratch assay and transwell assay were used to detect the cell proliferation, migration and invasion, respectively. Dual-luciferase reporter assay was used to verify the targeting relationship between miR-525-5p and SNHG17. Results Compared with the normal placenta tissues, the expression level of lncRNA SNHG17 in preeclampsia placenta tissues was increased, while the expression level of miR-525-5p was decreased (P< 0. 05). After transfection with si-SNHG17, the number of colonies and invasive cells were increased, the wound healing rate was increased (P< 0. 05). lncRNA SNHG17 could bind with miR-525-5p. After the co-transfection of si-SNHG17 and anti-miR-525-5p, the number of colonies and invasive cells were decreased, the wound healing rate was decreased ( P < 0. 05 ). Conclusion Silencing lncRNA SNHG17 could promote the proliferation, migration and invasion of human chorionic trophoblast cells by upregulation of miR-525-5p.

Key words: preeclampsia, human chorionic trophoblast cells, lncRNA SNHG17, miR-525- 5p, cell proliferation, migration, invasion