关键词: 霍奇金淋巴瘤, PCA3, miR-185, 细胞增殖, 凋亡

Abstract: Objective To investigate the effect of PCA3 on the proliferation and apoptosis of Hodgkin’s lymphoma cell line L428 and its possible mechanism. Methods Lymphoma tissues and normal adjacent tissues from 39 patients with Hodgkin’s lymphoma were collected. The expression levels of PCA3 and miR-185 in the tissues were dectected by RT-qPCR. L428 cells were divided into si-NC group, si-PCA3 group, si-PCA3 + NC group, si-PCA3 + miR-185 inhibitor group. CCK-8 was used to detect cell proliferation. Flow cytometry was used to detect cell cycle and apoptosis. Western blotting was used to detect the protein expression levels of pro-caspase 3 and cleaved-caspase 3. RT-qPCR was used to detect the expression levels of PCA3 and miR-185. The dual luciferase gene reporter assay was used to verify the regulatory relationship between PCA3 and miR-185. Results The expression level of PCA3 was increased and the expression level of miR-185 was decreased in the lymphoma tissues (P< 0. 05). Transfection of si-PCA3 could inhibit cell proliferation, arrest cell cycle, and promote cell apoptosis in L428 cells. PCA3 targets to the miR-185 and negatively regulate its expression. Co-transfection of si-PCA3 and miR-185 inhibitor could reduce the effect of transfection of si-PCA3 on cell proliferation, cell cycle and apoptosis. Conclusion Interfering the expression of PCA3 could target and negatively regulate miR-185 to inhibit the proliferation of lymphoma cells L428 and promote the apoptosis of L428 cells.

Key words: Hodgkin’s lymphoma, PCA3, miR-185, cell proliferation, apoptosis