关键词: 急性髓系白血病, 沉默信息调节因子 3, 细胞凋亡, 氧化应激, Wnt / β-catenin 信号通路

Abstract: Objective To explore the effect of silent information regulator 3 (SIRT3) in children with acute myeloid leukemia (AML). Methods The expression level of SIRT3 in bone marrow cells of AML children and peripheral blood leukocytes of healthy blood donor children was detected. SIRT3 expression was knocked down in AML cells. The cells’proliferation, apoptosis, oxidative stress indexes, and protein expression levels of the Wnt / β-catenin signaling pathway weredetected. Results Compared with those in the normal children group, the expression levels of SIRT3mRNA and protein were increased in the AML children group (P < 0. 05). After knocking down SIRT3expression in AML cells, the cells proliferation, the colony formation ability, the levels of glutathione (GSH) and superoxide dismutase (SOD), the total antioxidant capacity (T-AOC), the expression level of β-catenin, and the transcription activity of T cell factor / lymphocyte enhancer factor (TCF/ LEF) were decreased, while the apoptosis rate, the levels of reactive oxygen species (ROS), malondialdehyde (MDA) and phosphorylated glycogen synthase kinase 3β (p-GSK-3β) were increased (P<0. 05), the GSK-3β inhibitor TWS119 could partially reverse the above effects. Conclusion Knocking down SIRT3 can inhibit the growth of AML cells and increase oxidative stress level, which may berelated to the inhibition of Wnt / β-catenin signaling pathways.

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