白芍总苷改善百草枯致大鼠肺损伤及纤维化

doi:10.3870/j.issn.1672-8009.2026.04.010

摘要/Abstract

摘要： 目的探究白芍总苷对百草枯(PQ)诱导的大鼠肺损伤氧化应激和肺纤维化的影响及潜在作用机制。方法腹腔注射PQ诱导大鼠肺损伤,给予不同剂量白芍总苷干预,观察肺组织病理改变、氧化应激、炎症因子水平和纤维化相关因子mRNA水平、TGF-β1/Smad3信号通路蛋白表达。RLE-6TN细胞进行PQ处理并给予白芍总苷干预,观察细胞TGF-β1/Smad3通路相关分子表达。结果白芍总苷干预降低PQ大鼠肺损伤、MDA、HYP含量和血清TNF-α、IL-6、IL-1β、HYP含量,降低α-SMA、Collagen III、TIMP-1mRNA水平和TGF-β1、p-Smad3/Smad3表达,增加SOD、CAT含量和p-Smad7/Smad7表达,有剂量效应。白芍总苷干预提高PQ中毒RLE-6TN细胞p-Smad7表达,降低TGF-β1、p-Smad3表达。结论白芍总苷可改善PQ大鼠氧化应激及肺纤维化,其作用机制可能与抑制TGF-β1/Smad3信号通路有关。

关键词: 百草枯, 肺损伤, 白芍总苷, 氧化应激, 肺纤维化

Abstract: Objective To explore the effects of total glucosides of paeony(TGP)on oxidative stress and lung fibrosis in rats with paraquat(PQ)-induced lung injury. Methods Intraperitoneal injection of PQ was performed to induce lung injury in rats,then those rats were intervened with different doses of TGP.The pathological changes of lung tissues,oxidative stress,levels of inflammatory factors,mRNA levels of fibrosis-related factors and expressions of TGF-β1/Smad3 signaling pathway proteins were observed.RLE-6TN cells were treated with PQ and intervened with TGP.The expression levels of TGF-β1/Smad3 pathway related factors in cells were detected. Results After TGP intervention,the lung injury,the levels of MDA and HYP,the levels of serum TNF-α,IL-6 and IL-1β,the mRNA expression levels of α-SMA,Collagen Ⅲ and TIMP-1,and the expression levels of TGF-β1 and p-Smad3/Smad3 were decreased,while the levels of SOD and CAT,and the ratio of p-Smad7/Smad7 were increased,in a dose-dependent manner.After TGP intervention,the expression level of p-Smad7 in RLE-6TN cells was increased,while the expression levels of TGF-β1 and p-Smad3 were decreased. Conclusion TGP can improve oxidative stress and lung fibrosis in PQ rats.The mechanism may be related to TGF-β1/Smad3 signaling pathways.

Key words: paraquat, lung injury, total glucosides of paeonia, oxidative stress, lung fibrosis

中图分类号:

R322.3
R963

刘梅, 司永旺, 刘丽, 崔春叶, 王丹, 洛莹莹. 白芍总苷改善百草枯致大鼠肺损伤及纤维化[J]. 医学分子生物学杂志, 2026, 23(4): 437-444.

LIU Mei, SI Yongwang , LIU Li , CUI Chunye , WANG Dan , LUO Yingying. Improvement of Paraquat-induced Lung Injury and Fibrosis by Total Glucosides of Paeonia in Rats[J]. Journal of Medical Molecular Biology, 2026, 23(4): 437-444.

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