miR-370-3p靶向STAT3对肝癌细胞HepG2迁移、侵袭能力的影响

doi:10.3870/j.issn.1672-8009.2026.04.002

摘要/Abstract

摘要： 目的探讨miR-370-3p靶向调控STAT3对肝癌HepG2细胞转移能力的影响。方法将miR-NC或miR-370-3p mimic和pcDNA或pcDNA-STAT3分别转染到HepG2细胞,并检测miR-370-3p和STAT3 mRNA相对表达量。miR-370-3p mimic与STAT3-wild type(wt)或STAT3-mutant(mut)共转染HepG2细胞,最终建立对照组,miR-370-3p组,STAT3组和miR-370-3p+STAT3组。Transwell试验、划痕试验检测肿瘤细胞侵袭、迁移能力。蛋白质印迹检测上皮间质转化。建立原位异种移植肿瘤小鼠模型,分别为miR-NC转染组和miR-370-3p转染组。结果与对照组比较,miR-370-3p mimic组miR-370-3p mRNA相对表达量显著上调。pcDNA-STAT3组STAT3 mRNA相对表达量显著上调。miR-370-3p的过表达能抑制肝癌HepG2细胞侵袭、迁移并影响上皮间质转化相关蛋白的表达。同时miR-370-3p能靶向STAT3抑制肝癌HepG2细胞上述转移能力。在体内试验中miR-370-3p过表达能抑制肿瘤生长。结论 miR-370-3p能靶向STAT3抑制肝癌HepG2细胞侵袭、迁移。

关键词: miR-370-3p, STAT3, 肝癌, 侵袭, 迁移, 上皮间质转化

Abstract: Objective To explore the influence of miR-370-3p on migration and invasion of hepatocellular carcinoma HepG2 cells by targeting STAT3. Methods The miR-NC or miR-370-3p mimic and pcDNA or pcDNA-STAT3 were transfected into HepG2 cells,and the mRNA relative expression levels of miR-370-3p and STAT3 were detected.miR-370-3p mimic and STAT3-wild type(wt)or STAT3-mutant(mut)were co-transfected into HepG2 cells.Finally,the control group,miR-370-3p group,STAT3 group and miR-370-3p+STAT3 group were established.Transwell assay and Wound-healing assay were applied to detect the invasion,migration of cells.Western blot assay was applied to detect the expression level of epithelial-mesenchymal transition associated proteins.The orthotopic xenograft mouse model was established,and mice were divided into miR-NC group and miR-370-3p group. Results Compared with the control group,the relative expression level of miR-370-3p was significantly upregulated in the miR-370-3p mimic group.In the pcDNA-STAT3 group,the relative expression level of STAT3 mRNA was significantly upregulated.Overexpression of miR-370-3p inhibits invasion,migration,and affect the expression of epithelial-mesenchymal transition associated pproteins in HepG2 liver cancer cells.Additionally,miR-370-3p targets STAT3 to suppress the aforementioned metastatic capabilities of HepG2 liver cancer cells.In in vivo experiments,overexpression of miR-370-3p inhibits tumor growth. Conclusion miR-370-3p can inhibit the invasion,migration of hepatocellular carcinoma cells HepG2 by targeting STAT3.

Key words: miR-370-3p, STAT3, hepatocellular carcinoma, invasion, migration, epithelial-mesenchymal transition

中图分类号:

R735.7

刘雅婧, 贾仕婵, 姚书鹏. miR-370-3p靶向STAT3对肝癌细胞HepG2迁移、侵袭能力的影响[J]. 医学分子生物学杂志, 2026, 23(4): 382-388.

LIU Yajing, JIA Shichan, YAO Shupeng. Effect of miR-370-3p on Migration and Invasion of Hepatocellular Carcinoma Cells HepG2 via Targeting STAT3[J]. Journal of Medical Molecular Biology, 2026, 23(4): 382-388.

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