利福平调控miR-28-5p/Nrf2信号轴对肝细胞损伤的影响

doi:10.3870/j.issn.1672-8009.2026.04.011

摘要/Abstract

摘要： 目的探究利福平调控miR-28-5p/核转录因子红系2相关因子2(nuclear factor-erythroid 2-related factor 2,Nrf2)信号轴对肝细胞损伤的影响。方法将L02细胞分为6组:对照组,利福平低、中、高剂量组,miR NC组,miR-28-5p inhibitor组;随后检测各组L02细胞的miR-28-5p表达水平、活力、凋亡率、炎症因子水平、超氧化物歧化酶(superoxidedismutase,SOD)、丙二醛(malondialdehyde,MDA)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)水平。同时分析L02细胞中miR-28-5p与Nrf2的靶向关系。结果 L02细胞中的miR-28-5p表达水平随着利福平作用浓度的增加而增加。与对照组比较,利福平低、中、高剂量组与miR NC组和miR-28-5p inhibitor组的L02细胞的活力降低,凋亡率增加,炎症因子水平增加,MDA、AST及ALT水平增加,SOD与Nrf2水平降低。与miR NC组比较,miR-28-5p inhibitor组的L02细胞的活力增加,凋亡率降低,炎症因子水平降低,MDA、AST及ALT水平降低,SOD与Nrf2水平增加。miR-28-5p mimic能够靶向抑制Nrf2的表达。结论利福平能够通过激活miR-28-5p抑制Nrf2的表达进而促进肝细胞损伤。

Abstract: Objective To explore the effect of rifampicin on miR-28-5p/nuclear factor-erythroid 2-related factor 2(Nrf2)signaling and liver cell injury. Methods L02 cells were divided into 6 groups:control group,low-,medium- ,and high-dose rifampicin groups,miR NC group,and miR-28-5p inhibitor group.The expression level of miR-28-5p,cell viability,apoptosis rate,inflammatory factor levels,superoxide dismutase(SOD),malondialdehyde(MDA),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)in each group of L02 cells were detected.Meanwhile,the targeting relationship between miR-28-5p and Nrf2 in L02 cells was analyzed. Results The expression level of miR-28-5p in L02 cells were increased with the increase of rifampicin concentration.Compared with those in the control group,the viability of L02 cells in the low-,medium-,and high-dose rifampicin groups,miR NC group,and miR-28-5p inhibitor group was decreased,the apoptosis rate was increased,the levels of inflammatory factors,MDA,AST,and ALT were increased,and the levels of SOD and Nrf2 were decreased.Compared with those in the miR NC group,the viability of L02 cells in the miR-28-5p inhibitor group was increased,the apoptosis rate was decreased,the levels of inflammatory factors,MDA,AST,and ALT were decreased,and the levels of SOD and Nrf2 were increased.miR-28-5p could targetly inhibit the expression of Nrf2. Conclusion Rifampicin can promote liver cell injury by miR-28-5p and inhibition of Nrf2.

Key words: nuclear factor-erythroid 2-related factor 2, rifampicin, miR-28-5p, apoptosis, hepatocyte injury

中图分类号:

R575.3

陈振华, 王林, 梅湘, 邹芳芳. 利福平调控miR-28-5p/Nrf2信号轴对肝细胞损伤的影响[J]. 医学分子生物学杂志, 2026, 23(4): 445-451.

CHEN Zhenhua, WANG Lin, MEI Xiang, ZOU Fangfang. Effect of Rifampicin on Hepatocyte Injury by Regulation of miR-28-5p and Nrf2[J]. Journal of Medical Molecular Biology, 2026, 23(4): 445-451.

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