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Abstract: Objective To investigate the mechanism by which Wutou decoction ( WTD) inhibits inflammatory and oxidative stress induced injury of human urogenic stem cells ( UdSC) bymodulating the aryl hydrocarbon receptor ( Ahr ) / long non-coding RNA ( LncRNA )LOC101928120 / SHC articulin 1 ( SHC1) signaling pathway. Methods UdSC cells were dividedinto 5 groups Control group, IL-1β group, IL-1β + WTD group, H2 O2 group, and H2 O2 + WTD group. CellTiterGlo assay and flow cytometry were applied to detect the proliferation and apoptosis in the cells of each group. qRT-PCR was applied to detect the expression levels of Ahr, LOC101928120, and SHC1 mRNA. Western blotting was applied to detect the expression levels of Ahr, HDAC1, SHC1, COL2, and SOX-9. ELISA was applied to detect the levels of IL-6, TNF-α, and MMP-13 in inflammation-induced UdSC cells. DCFH-DA fluorescent probe was applied todetect the ROS level in oxidative stress-induced UdSC cells. Results The A 560 nmvalue, the expression levels of Ahr mRNA and protein, LOC101928120, COL2, SOX-9 protein in the IL-1β group were lower than those in the Control group, and the values of above indexes in the IL-1β + WTDgroup were higher than those in the IL-1β group (P< 0. 05). The apoptosis rate, the levels of IL-6,TNF-α, MMP-13, and the expression levels of SHC1 mRNA and protein and HDAC1 protein in the IL-1β group were higher than those in the control group, and the values of above indexes in the IL-1β + WTD group were lower than those in the IL-1β group (P < 0. 05). TheA560 nm value, the expression levels of Ahr mRNA and protein, LOC101928120, COL2 and SOX-9 proteins in the H2O2 group were lower than those in the Control group, and the values of above indexes in the H2 O2 + WTD group were higher than those in the H2O2 group (P< 0. 05). The apoptosis rate, the proportion of ROS-positive cells, and the expression levels of SHC1 mRNA and protein and HDAC1 protein were higher than those in the Control group, and the above values in the H2 O2 + WTD groupwere lower than those in H 2O2 group (P< 0. 05). Conclusion WTD inhibits inflammatory and oxidative stress-induced injury in human UdSC, the mechanism may be achieved by modulating the Ahr / LOC101928120 / SHC1 signaling pathway.

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