LINC00659 靶向 miR-149-5p 调控食管鳞癌细胞 Eca-109 的增殖、
迁移、 侵袭及放射敏感性

doi:10.3870/j.issn.1672-8009.2022.02.012

摘要/Abstract

摘要： 目的探讨长基因间非编码 RNA 00659 (LINC00659) 是否靶向 miR-149-5p 调控食管鳞癌 Eca109 细胞的增殖、迁移侵袭及放射敏感性。方法实时定量 PCR (RT-qPCR) 分析 30 对食管鳞癌组织、对照组织中 LINC00659 和 miR-149-5p 表达量。将 Eca-109 细胞分为 si-NC 组、 si-LINC00659 组、 miR-NC 组、 miR-149-5p、 si-LINC00659 + anti-miR-NC 组、 si-LINC00659 + anti-miR-149-5p 组。 CCK-8 法分析 LINC00659 和 miR-149-5p 表达对 Eca-109 细胞增殖的影响, Transwell 迁移/ 侵袭实验分析 LINC00659 和 miR-149-5p 表达对 Eca-109 细胞迁移和侵袭的影响。克隆形成实验检测 Eca-109 细胞存活分数和放射增敏比。双荧光素酶报告实验分析 LINC00659 与 miR-149-5p 的关系。结果与癌旁组织比较, 食管鳞癌组织中 LINC00659 表达量显著升高, 而 miR-149-5p 表达量显著降低 (P< 0. 05)。与 si-NC 组比较, si-LINC00659 组 Eca-109 细胞增殖抑制率升高, 迁移和侵袭数、细胞存活分数降低 (P< 0. 05), 放射增敏比为 1. 938。与 miR-NC 组比较, miR-149-5p 组 Eca-109 细胞增殖抑制率升高 [ (35. 32 ± 1. 72) % vs. (5. 68 ± 0. 46) % ], 迁移和侵袭数、细胞存活分数降低 (P< 0. 05), 放射增敏比为 1. 545。 miR-149-5p 是 LINC00659 的靶基因。与 si-LINC00659 + anti-miR-NC 组比较, si-LINC00659 + anti-miR-149-5p 组 Eca-109 细胞增殖抑制率降低, 迁移和侵袭数、细胞存活分数升高 (P< 0. 05), 放射增敏比为 0. 657。结论干扰 LINC00659 通过上调 miR-149-5p 能够抑制食管鳞癌 Eca-109 细胞的增殖、迁移侵袭, 并提高其放射敏感性。

关键词: LINC00659, 食管鳞癌, 增殖, 迁移, 侵袭, 放射敏感性, miR-149-5p

Abstract: Objective To investigate whether long intergenic non-coding RNA 00659 (LINC00659) targets miR-149-5p to regulate the proliferation, migration, invasion and radiosensitivity of esophageal squamous cell carcinoma Eca-109 cells. Methods The expression of LINC00659 and miR-149-5p in 30 pairs of esophageal squamous cell carcinoma tissues and control tissues was analyzed using real-time quantitative PCR (RT-qPCR). Eca-109 cells cultured in vitro were divided into si-NC group, si-LINC00659 group, miR-NC group, miR-149-5p group, siLINC00659 + anti-miR-NC group, and si-LINC00659 + anti-miR-149-5p group. The effects of LINC00659 and miR-149-5p on the proliferation of Eca-109 cells were measured by CCK-8 cell viability assay. The effects of LINC00659 and miR-149-5p on the migration and invasion of Eca-109 cells were determined through Transwell migration / invasion assay. The colony formation test was used to measure the survival fraction and radiosensitization ratio of Eca-109 cells. The relationship between LINC00659 and miR-149-5p was verified by dual luciferase report experiment. Results The expression level of LINC00659 was significantly higher in the esophageal squamous cell carcinoma tissue than in the control group, while the expression level of miR-149-5p was significantly lower in the esophageal squamous cell carcinoma tissue than in control tissues (P< 0. 05). Compared with the si-NC group, the proliferation inhibition rate of Eca-109 cells in the si-LINC00659 group was increased, and the number of migratory / invasive cells and the cell survival fraction were decreased (P< 0. 05). The radiosensitization ratio of Eca-109 cells in the si-LINC00659 group was 1. 938. In the miR-149-5p group relative to the miR-NC group, the proliferation inhibition rate of Eca-109 cells was increased, and the number of migratory / invasive cells and the cell survival fraction were decreased (P< 0. 05). The radiosensitization ratio of Eca-109 cells in the miR-149-5p group was 1. 545. miR-149-5p was the target gene of LINC00659. Compared with the si-LINC00659 + anti-miRNC group, the proliferation inhibition rate of Eca-109 cells in si-LINC00659 + anti-miR-149-5p group was decreased, and the number of migratory / invasive cells and the cell survival fraction were increased (P < 0. 05). The radiosensitization ratio of Eca-109 cells in si-LINC00659 + anti-miR149-5p group was 0. 657. Conclusion The interference of LINC00659 inhibits cell proliferation, migration and invasion, improves cell radiosensitivity of esophageal squamous cell carcinoma Eca109 cells by up-regulating miR-149-5p.

Key words: LINC00659, esophageal squamous cell carcinoma cancer, proliferation, migration, invasion, radiosensitivity, miR-149-5p

中图分类号:

R735. 1

雷乘强, 文光瑞, 昌建波. LINC00659 靶向 miR-149-5p 调控食管鳞癌细胞 Eca-109 的增殖、迁移、侵袭及放射敏感性 [J]. 医学分子生物学杂志, 2022, 19(2): 163-170.

LEI Chengqiang, WEN Guangrui, CHANG Jianbo. LINC00659 Regulates the Proliferation, Migration, Invasion and Radiosensitivity of Esophageal Squamous Cell Carcinoma Eca-109 Cells by Targeting mir-149-5p[J]. Journal of Medical Molecular Biology, 2022, 19(2): 163-170.

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LINC00659 靶向 miR-149-5p 调控食管鳞癌细胞 Eca-109 的增殖、迁移、侵袭及放射敏感性

LINC00659 Regulates the Proliferation, Migration, Invasion and Radiosensitivity of Esophageal Squamous Cell Carcinoma Eca-109 Cells by Targeting mir-149-5p

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LINC00659 靶向 miR-149-5p 调控食管鳞癌细胞 Eca-109 的增殖、 迁移、 侵袭及放射敏感性

LINC00659 Regulates the Proliferation, Migration, Invasion and Radiosensitivity of Esophageal Squamous Cell Carcinoma Eca-109 Cells by Targeting mir-149-5p

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LINC00659 靶向 miR-149-5p 调控食管鳞癌细胞 Eca-109 的增殖、迁移、侵袭及放射敏感性