关键词: miR-592, PR 结构域锌指蛋白 5, 肾细胞癌, 侵袭转移, 自噬

Abstract: Objective To explore the effect of miR-592 on the migration, invasion and autophagy of renal cell carcinoma ( RCC) via targeting PR domain zinc finger protein 5 ( PRDM5).Methods RT-qPCR and Western blotting were used to detect the expression levels of miR-592 andPRDM5 mRNA and protein in RCC tissues and cells. A498 and 786-O cells were divided into 4groups: anti-miR-NC group, anti-miR-592 group, anti-miR-592 + si-NC group and anti-miR-592 + si-PRDM5 group. RNA immunoprecipitation (RIP) was used to determine the combination of miR-592 and PRDM5. CCK-8, wound-healing assay and transwell test were used to analyse the cell proliferation, migration and invasion. Transmission electron microscopy was used to observe the autophagosome formation. Western blotting was used to detect the expression levels of PRDM5, matrix metalloproteinase ( MMP ) -2, MMP-9, Beclin1, microtubule-associated protein 1 light chain 3( LC3) Ⅱ / Ⅰ . Nude mice model of transplanted tumors was established to analyze the effect of knocking down miR-592 on the growth of transplanted tumors. Results PRDM5 mRNA and protein expression levels in the RCC tissues and cells were decreased (P< 0. 05). After knocking down the expression of miR-592, the cell A450 value, the number of migrated and invaded cells, the expression levelsof MMP-2 and MMP-9 were decreased, while the number of autophagosomes, the expression levels ofBeclin1 and LC3 II / I were increased (P< 0. 05). Knocking down the expression of PRDM5 could partially reverse the effect of miR-592 knock-down on cell proliferation, invasion and autophagy (P <0. 05). Knocking down the expression of miR-592 could inhibit the growth of transplanted tumors innude mice (P<0. 05). Conclusion miR-592 promotes the proliferation, invasion and migration ofRCC cells, and inhibits autophagy by targeting the expression of PRDM5.

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