丹皮酚对慢性不可预知温和应激诱导的抑郁症大鼠空间学习记忆和抑郁样行为的影响及机制

doi:10.3870/j.issn.1672-8009.2026.04.009

医学分子生物学杂志 ›› 2026, Vol. 23 ›› Issue (4): 431-436.doi: 10.3870/j.issn.1672-8009.2026.04.009

丹皮酚对慢性不可预知温和应激诱导的抑郁症大鼠空间学习记忆和抑郁样行为的影响及机制

李卫¹, 毕翻¹, 王凯¹, 曾云², 郭志伟³

达州中医药职业学院¹基础医学教育部,²针灸康复学院四川省达州市,635000
³南充市中心医院影像科四川省南充市,637000

收稿日期:2025-12-04 发布日期:2026-09-28
通讯作者: 毕翻(E-mail:bifan110411@163.com)
基金资助:
四川省医学(青年创新)科研项目(No.Q20043)

Effect and Mechanism of Paeonol on Spatial Learning,Memory and Depression-Like Behavior in Rats with Chronic Unpredictable Mild Stress-induced Depression

LI Wei¹, BI Fan¹, WANG Kai¹, ZENG Yun², GUO Zhiwei³

¹Department of Basic Medical Education,²College of Acupuncture and Rehabilitation,Dazhou Vocational College of Chinese Medicine,Dazhou,Sichuan,635000,China
³Department of Imaging,Nanchong Central Hospital,Nanchong,Sichuan,637000,China

Received:2025-12-04 Published:2026-09-28
Contact: BI Fan(E-mail:bifan110411@163.com)
Supported by:
Sichuan Province Medical (Youth Innovation) Research Project (No. Q20043)

摘要/Abstract

摘要： 目的分析丹皮酚对慢性不可预知温和应激(chronic unpredictable mild stress,CUMS)诱导的抑郁症大鼠空间学习记忆和抑郁行为的影响,并探讨其作用机制。方法通过CUMS诱导抑郁症大鼠模型并分为抑郁症组,丹皮酚低、中、高剂量组和Flu组,应激第3周时给予药物干预,持续1周;对照组大鼠不做处理。进行Morris水迷宫、糖水偏爱、强迫游泳实验(forced swimming test,FST)和悬尾实验(tail suspension test,TST),检测血清CRH、ACTH、CORT水平及海马组织ERK/CREB蛋白表达。结果与对照组比较,抑郁症组大鼠糖水偏爱度降低(P<0.05),逃避潜伏期延长,穿越平台次数及第三象限停留时间减少(P<0.05),FST及TST中不动时间增加(P<0.05),血清CRH、ACTH及CORT水平升高(P<0.05),海马组织p-ERK/ERK、p-CREB/CREB比值降低(P<0.05);与抑郁症组比较,中、高剂量丹皮酚组和Flu组上述指标均明显逆转(P<0.05)。结论丹皮酚能改善CUMS诱导的抑郁症大鼠空间学习记忆能力和抑郁样行为,其机制与调节HPA轴以及激活ERK/CREB信号通路有关。

关键词: 抑郁症, 慢性不可预知温和应激, 丹皮酚, 细胞外信号调节激酶, 环磷酸腺苷反应元件结合蛋白

Abstract: Objective To analyze the effect of paeonol on spatial learning,memory and depression-like behavior in rats with chronic unpredictable mild stress(CUMS)-induced depression,and to explore its mechanism. Methods The CUMS-induced depression rats were divided into 5 groups:depression group,low-dose,medium-dose and high-dose paeonol groups and Flu group,and the control group was set up.Drug intervention was administered during the third week of stress and continued for one week;rats in the control group received no treatment.The Morris water maze,sucrose preference test,forced swimming test(FST)and tail suspension test(TST)were conducted.The levels of serum CRH,ACTH and CORT,and the expression levels of ERK/CREB proteins in hippocampus were detected. Results Compared with those in the control group,the sucrose preference of rats in the depression group was declined(P<0.05),the escape latency was prolonged,the times of platform crossings and residence time in the third quadrant were decreased(P<0.05),the immobility time in the FST and TST was increased(P<0.05),the levels of serum CRH,ACTH and CORT were increased(P<0.05),and ratios of p-ERK/ERK and p-CREB/CREB in the hippocampus were decreased(P<0.05).Compared with those in the depression group,the above indexes were significantly reversed in rats of the medium- and high-dose paeonol groups and the Flu group(P<0.05). Conclusion Paeonol can improve spatial learning,memory ability and depression-like behavior in rats with CUMS-induced depression,and the mechanism is related to regulation of HPA and ERK/CREB signaling pathways.

Key words: depression, chronic unpredictable mild stress, paeonol, extracellular signal-regulated kinase, cAMP-response element binding protein

中图分类号:

R285.5

李卫, 毕翻, 王凯, 曾云, 郭志伟. 丹皮酚对慢性不可预知温和应激诱导的抑郁症大鼠空间学习记忆和抑郁样行为的影响及机制[J]. 医学分子生物学杂志, 2026, 23(4): 431-436.

LI Wei, BI Fan, WANG Kai, ZENG Yun, GUO Zhiwei. Effect and Mechanism of Paeonol on Spatial Learning,Memory and Depression-Like Behavior in Rats with Chronic Unpredictable Mild Stress-induced Depression[J]. Journal of Medical Molecular Biology, 2026, 23(4): 431-436.

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丹皮酚对慢性不可预知温和应激诱导的抑郁症大鼠空间学习记忆和抑郁样行为的影响及机制

Effect and Mechanism of Paeonol on Spatial Learning,Memory and Depression-Like Behavior in Rats with Chronic Unpredictable Mild Stress-induced Depression

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