lncRNA NCK1-AS1低表达阻碍甲状腺癌细胞CAL-62的生长和侵袭

doi:10.3870/j.issn.1672-8009.2026.04.008

摘要/Abstract

摘要： 目的探讨干扰长链非编码RNA lncRNA NCK1-AS1表达对甲状腺癌细胞CAL-62生长和侵袭的影响。方法转染相应shRNA干扰CAL-62细胞中lncRNA NCK1-AS1表达,RT-PCR检测lncRNA NCK1-AS1表达水平。EdU染色检测细胞增殖,流式检测细胞凋亡。RT-PCR检测增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、p21、生存素(Survivin)的mRNA表达水平。Transwell法检测细胞侵袭,微管形成实验检测微管样结构数。蛋白质印迹检测血管内皮生长因子(vascular endothelial growth factor,VEGF)、E-钙黏蛋白(epithelial cadherin,E-cadherin)、N-钙黏蛋白(neural cadherin,N-cadherin)表达水平。结果与对照组比较,lncRNA NCK1-AS1干扰组细胞增殖率降低,凋亡细胞增多,PCNA和Survivin mRNA水平下调,p21 mRNA水平上调,侵袭细胞和微管样结构数减少,VEGF、N-cadherin蛋白表达下调,E-cadherin表达上调,差异具有统计学意义(P<0.05)。结论干扰lncRNA NCK1-AS1表达能够抑制甲状腺癌细胞CAL-62生长和侵袭。

关键词: lncRNA NCK1-AS1, CAL-62细胞, 细胞生长, 侵袭

Abstract: Objective To investigate the effects of interfering with the expression of the lncRNA NCK1-AS1 on the growth and invasion of the thyroid cancer cell line CAL-62. Methods lncRNA NCK1-AS1 expression in CAL-62 cells was silenced by transfecting the corresponding shRNA.The expression level of lncRNA NCK1-AS1 was detected by RT-PCR.Cell proliferation was assessed by EdU staining,and apoptosis was analyzed by flow cytometry.RT-PCR was used to detect the mRNA expression levels of proliferating cell nuclear antigen(PCNA),p21,and Survivin.Cell invasion was assessed using the Transwell assay,and the number of microtubule-like structures was measured via a microtubule formation assay.Western blot analysis was performed to detect the expression levels of vascular endothelial growth factor(VEGF),E-cadherin,and N-cadherin. Results Compared with the control group,the lncRNA NCK1-AS1 interference group exhibited reduced cell proliferation,increased apoptosis,downregulated PCNA and Survivin mRNA levels,upregulated P21 mRNA levels,decreased number of invading cells and microtubule-like structures,downregulated VEGF and N-cadherin protein expression,and upregulated E-cadherin expression.These differences were statistically significant(P<0.05). Conclusion Silencing the expression of lncRNA NCK1-AS1 inhibits the growth and invasion of CAL-62 thyroid cancer cells.

Key words: lncRNA NCK1-AS1, CAL-62 cells, cell growth, exercise capacity

中图分类号:

王丹, 王琼, 赵茜, 许琳, 刘天宇, 孙吉瑞. lncRNA NCK1-AS1低表达阻碍甲状腺癌细胞CAL-62的生长和侵袭[J]. 医学分子生物学杂志, 2026, 23(4): 424-430.

WANG Dan, WANG Qiong, ZHAO Qian, XU Lin, LIU Tianyu, SUN Jirui. Interference of lncRNA NCK1-AS1 Experssion Inhibits Growth and Invasion of CAL-62 Thyroid Cancer Cells[J]. Journal of Medical Molecular Biology, 2026, 23(4): 424-430.

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