miR-429通过Wnt/β-catenin通路影响慢性难愈合创面修复

doi:10.3870/j.issn.1672-8009.2026.04.005

医学分子生物学杂志 ›› 2026, Vol. 23 ›› Issue (4): 406-411.doi: 10.3870/j.issn.1672-8009.2026.04.005

miR-429通过Wnt/β-catenin通路影响慢性难愈合创面修复

黄文文¹, 孟丽², 谢辉¹, 胡寿勇¹, 石华峰¹

荆门市中心医院/荆楚理工学院附属荆门市中心医院¹创伤骨科,²内分泌科湖北省荆门市,448001

收稿日期:2025-12-09 发布日期:2026-09-28
通讯作者: 黄文文(E-mail:hwwen15327536882@163.com)
基金资助:
荆门市科学技术局项目(No.2022YFZD218)

miR-429 Affects Chronic Non-Healing Wound Repair through Wnt/β-catenin Pathway

HUANG Wenwen¹, MENG Li², XIE Hui¹, HU Shouyong¹, SHI Huafeng¹

¹Department of Trauma and Orthopedics,²Department of Endocrinology,Jingmen Central Hospital/Jingmen Central Hospita Affiliated to Jingchu University of Technology,Jingmen,Hubei,448001,China

Received:2025-12-09 Published:2026-09-28
Contact: HUANG Wenwen(E-mail:hwwen15327536882@163.com)
Supported by:
Jingmen Municipal Bureau of Science and Technology (No. 2022YFZD218)

摘要/Abstract

摘要： 目的探究miR-429对慢性难愈合创面修复及Wnt/β-catenin信号通路的影响。方法将60大鼠随机分为空白对照组、模型组、阴性对照组(注射antagomir NC)、LV-429组(注射miR-429 antagomir慢病毒)和抑制剂组(注射miR-429 antagomir慢病毒+Wnt/β-catenin信号通路抑制剂IWP-2)。统计大鼠创面愈合率,取创面组织检测miR-429水平,Wnt3a和β-catenin蛋白表达量,血管内皮生长因子A(vascular endothelial growth factor A,VEGFA),内皮型一氧化氮合酶(endothelial nitric oxide synthase,ENOS)表达情况。取大鼠血清检测白介素6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平。结果与模型组和阴性对照组比较,LV-429组和抑制剂组miR-429表达下降。与模型组和阴性对照组比较,LV-429组换药第3、6、12天时创面愈合率,Wnt3a、β-catenin的蛋白表达,VEGFA、ENOS阳性表达率均上升,IL-6和TNF-α水平均下降(P<0.05),抑制剂组创面愈合率,Wnt3a、β-catenin的蛋白表达,VEGFA、ENOS阳性表达率均下降,IL-6和TNF-α水平均上升(P<0.05)。结论抑制miR-429的表达可促进慢性难愈合创面修复,该过程通过激活Wnt/β-catenin信号通路介导。

关键词: miR-429, 创面修复, Wnt/β-catenin通路

Abstract: Objective To investigate the effects of miR-429 on the healing of chronic non-healing wounds and the Wnt/β-catenin signaling pathway. Methods A total of 60 rats were randomly divided into 5 groups:blank control group,model group,negative control group(injected with antagomir NC),LV-429 group(injected with miR-429 antagomir lentivirus),and inhibitor group(injected with miR-429 antagomir lentivirus and Wnt/β-catenin signaling pathway inhibitor IWP-2).The wound healing rates in rats were assessed,and wound tissue samples were collected for measurement of miR-429 levels,as well as the protein expression of Wnt3a and β-catenin,and the expression of vascular endothelial growth factor A(VEGFA)and endothelial nitric oxide synthase(ENOS).Rat serum samples were collectedor measurement of the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α). Results Compared with that in the model group and the negative control group,the miR-429 expression level was reduced in the LV-429 group and the inhibitor group.Compared with the model group and the negative control group,in the LV-429 group,the wound healing rates,Wnt3a,β-catenin protein expression,and the positive expression rates of VEGFA and ENOS were all increased,while the IL-6 and TNF-α levels were both decreased(P<0.05).In the inhibitor group,the wound healing rate,Wnt3a and β-catenin protein expression,and the positive expression rates of VEGFA and ENOS were all decreased,while the IL-6 and TNF-α levels were both increased(P<0.05). Conclusion Inhibiting the expression of miR-429 can promote the repair of chronic refractory wounds,and this process is mediated by activating the Wnt/β-catenin signaling pathway.

Key words: miR-429, wound repair, Wnt/β-catenin signaling pathway, vascular endothelial growth factor A, endothelial nitric oxide synthase, wound healing rate, interleukin-6, tumor necrosis factor-α

中图分类号:

R339.1

黄文文, 孟丽, 谢辉, 胡寿勇, 石华峰. miR-429通过Wnt/β-catenin通路影响慢性难愈合创面修复[J]. 医学分子生物学杂志, 2026, 23(4): 406-411.

HUANG Wenwen, MENG Li, XIE Hui, HU Shouyong, SHI Huafeng. miR-429 Affects Chronic Non-Healing Wound Repair through Wnt/β-catenin Pathway[J]. Journal of Medical Molecular Biology, 2026, 23(4): 406-411.

参考文献

[1] 李晓辉,黄象艳.自体富血小板血浆在慢性难愈合创面治疗中的应用进展[J].山东医药,2023,63(4):107-110.
LI X H,HUANG X Y.Application progress of autologous platelet-rich plasma in the treatment of chronic refractory wounds[J].Shandong Med J,2023,63(4):107-110.
[2] WILSON M.Theuse of a synthetic hybrid-scale fiber matrix to treat difficult-to-heal wounds[J].Cureus,2023,15(12):e50405.
[3] 马慧可,李萍,陈佳,等.慢性难愈合创面“缸口”的形成机制和中医药治疗研究进展[J].医学研究杂志,2023,52(7):194-197.
MA H K,LI P,CHEN J,et al.Research progress on the formation mechanism and treatment of chronic refractory wounds with traditional Chinese medicine[J].J Med Res,2023,52(7):194-197.
[4] 熊元,曹发奇,刘梦非,等.2023创伤骨科慢性难愈性创面诊疗指南:治疗概要[J].临床外科杂志,2024,32(1):49-52.
XIONG Y,CAO F Q,LIU M F,et al.Trauma orthopedics chronic non-healing wound diagnosis and treatment guidelines 2023:Overview of treatment[J].J Clin Surg,2024,32(1):49-52.
[5] DORMERK J,GKOTSOULIAS E.The role of hemodynamic shear stress in healing chronic wounds[J].Wounds,2022,34(11):254-262.
[6] 张珂珂,黄国宝.miR-429对烧伤小鼠肺组织炎性反应和氧化应激的影响[J].河北医药,2024,46(3):330-334.
ZHANG K K,HUANG G B.Effect of miR-429 on inflammatory response and oxidative stress in lung tissue of burned mice[J].Hebei Med J,2024,46(3):330-334.
[7] 徐凤英,梁萍萍,廖若夷.基于Wnt/β-catenin信号通路探讨益气生肌合剂对慢性难愈性创面大鼠模型修复愈合的影响及作用机制[J].环球中医药,2023,16(3):453-459.
XU F Y,LIANG P P,LIAO R Y.To investigate the effect and mechanism of Yiqi Shengji mixture on the repair and healing of chronic refractory wound in rats based on Wnt/β-catenin signaling pathway[J].Glob Tradit Chin Med,2023,16(3):453-459.
[8] 左远娟,黄金梅,丘平,等.基于Wnt/β-catenin信号通路探讨MEBT/MEBO促进慢性难愈合创面愈合的机制[J].现代中西医结合杂志,2024,33(12):1622-1628.
ZUO Y J,HUANG J M,QIU P,et al.MEBT/MEBO promotes the healing of chronic refractory wounds via Wnt/β-catenin signaling pathway[J].Mod J Integr Tradit Chin West Med,2024,33(12):1622-1628.
[9] 张小峰.体表慢性难愈性创面住院患者的回顾性分析[D].西安:西安医学院,2023.
ZHANG X F.Retrospective analysis of inpatients with chronic refracyory surface wounds[D].Xi’an:Xi’an Medical University,2023.
[10] LIU W,GAO R,YANG C,et al.ECM-mimetic immunomodulatory hydrogel for methicillin-resistant Staphylococcus aureus-infected chronic skin wound healing[J].Sci Adv,2022,8(27):eabn7006.
[11] 骆渊城,朱滨,杨威,等.益气活血生肌方对难愈性创面愈合及血管再生的影响[J].中国医药导报,2023,20(12):30-34.
LUO Y C,ZHU B,YANG W,et al.Effect of Yiqi Huoxue Shengji Prescription on refractory wound healing and vessel regeneration[J].China Med Her,2023,20(12):30-34.
[12] SUN Y,DING S,FAN Y,et al.miR-429 inhibits the angiogenesis of human brain microvascular endothelial cells through SNAI2-mediated GSK-3β/β-catenin pathway[J].Comput Math Methods Med,2021,2021:6753926.
[13] CHEN S,WANG J,WANG J,et al.Wnt/β-catenin signaling pathway promotes abnormal activation of fibroblast-like synoviocytes and angiogenesis in rheumatoid arthritis and the intervention of Er Miao San[J].Phytomedicine,2023,120:155064.
[14] LIU H,ZHANG H,LIU X,et al.Pancreatic stellate cells exploitWnt/β-catenin/TCF7-mediated glutamine metabolism to promote pancreatic cancer cells growth[J].Cancer Lett,2023,555:216040.
[15] 肖雪飞,刘石,符艳,等.黄芪甲苷通过调控Wnt/β-catenin信号通路对人成纤维细胞凋亡和肌成纤维细胞转化的作用[J].湖南中医药大学学报,2024,44(12):2183-2192.
XIAO X F,LIU S,FU Y,et al.Effects of astragaloside-Ⅳ on human fibroblast apoptosis and myofibroblast transformation by regulating Wnt/β-catenin signaling pathway[J].J Tradit Chin Med Univ Hunan,2024,44(12):2183-2192.
[16] ZHANG L,LIU Q,MU Q,et al.miR-429 suppresses proliferation and invasion of breast cancer via inhibiting the Wnt/β-catenin signaling pathway[J].Thorac Cancer,2020,11(11):3126-3138.
[17] KHALED M L,BYKHOVSKAYA Y,YABLONSKI S E R,et al.Differential expression of coding and long noncoding RNAs in keratoconus-affected corneas[J].Invest Ophthalmol Vis Sci,2018,59(7):2717-2728.
[18] LI B,ZHANG Y,YIN R,et al.Activating CD137 signaling promotes sprouting angiogenesis via increased VEGFA secretion and the VEGFR2/Akt/ENOS pathway[J].Mediators Inflamm,2020,2020:1649453.
[19] 王双勋,李大勇.基于Wnt/β-catenin通路探讨拔毒生肌散对糖尿病大鼠皮肤溃疡愈合的促进作用[J].中华中医药学刊,2023,41(2):115-119,266-267.
WANG S X,LI D Y.Promoting effect of Badu Shengji Powder on skin ulcer healing in diabetic rats based on Wnt/β-catenin pathway[J].Chin Arch Tradit Chin Med,2023,41(2):115-119,266-267.
[20] YANG Y,HUANG P,YANG J,et al.Therapeutic effect and concomitant toxicity of hydrargyrumchloratum compositum on chronic difficult-to-heal wounds in rats[J].J Ethnopharmacol,2025,347:119749.

miR-429通过Wnt/β-catenin通路影响慢性难愈合创面修复

miR-429 Affects Chronic Non-Healing Wound Repair through Wnt/β-catenin Pathway

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