基于Wnt信号通路分析ULK1对子痫前期大鼠胎盘滋养层细胞侵袭、迁移的影响

doi:10.3870/j.issn.1672-8009.2026.04.006

摘要/Abstract

摘要： 目的基于Wnt通路分析Unc-51样激酶1(unc-51 like kinase 1,ULK1)对子痫前期大鼠胎盘滋养层细胞侵袭、迁移的影响。方法建立子痫前期大鼠模型后从胎盘组织分离滋养层细胞,将子痫前期滋养层细胞随机分为4个实验组:阴性对照组、siRNA ULK1组、SKL2001组、siRNA ULK1+KYA1797K组。CCK8实验检测滋养层细胞的增殖活力;Transwell实验检测滋养层细胞的侵袭与迁移能力;流式细胞术检测滋养层细胞的凋亡率;蛋白质印迹法检测滋养层细胞中ULK1及Wnt通路蛋白的表达。结果 siRNA ULK1组、siRNA ULK1+KYA1797K组滋养层细胞的ULK1表达明显降低,而SKL2001组滋养层细胞的ULK1表达无明显变化。siRNA ULK1以及SKL2001组的滋养层细胞增殖活力升高,侵袭及迁移能力增强,凋亡率降低,Wnt3α、β-catenin、c-Myc、Cyclin D1蛋白表达上调。siRNA ULK1+KYA1797K组滋养层细胞的增殖活力、侵袭、迁移能力、细胞凋亡率、Wnt3α和β-catenin以及c-Myc与Cyclin D1蛋白表达相较于阴性对照组无明显变化。结论抑制ULK1表达后,子痫前期大鼠滋养层细胞的增殖、迁移与侵袭活性增加,凋亡率降低,该过程与Wnt/β-catenin信号通路激活相关。

关键词: Unc-51样激酶1, Wnt信号通路, 滋养层细胞, 侵袭, 迁移, 凋亡

Abstract: Objective The effect of unc-51 like kinase 1(ULK1)on the invasion and migration of trophoblastic cells in preeclampsia rats was analyzed based on the Wnt signaling pathway. Methods After establishing a preeclampsia rat model,trophoblastic cells were isolated from placental tissues.Preeclampsia trophoblastic cells were randomly divided into 4 groups:negative control group,siRNA ULK1 group,SKL2001 group,and siRNA ULK1+KYA1797K group.The proliferation activity of trophoblastic cells was detected by CCK8 assay;the invasion and migration abilities of trophoblastic cells were detected by Transwell assay;the apoptosis rate of trophoblastic cells was detected by flow cytometry;the expression levels of ULK1 and Wnt pathway proteins in trophoblastic cells were analyzed by Western blotting. Results The expression level of ULK1 in trophoblastic cells of the siRNA ULK1 group and the siRNA ULK1+KYA1797K group was significantly decreased,while the expression level of ULK1 in the SKL2001 group showed no significant change.The proliferation activity of trophoblastic cells in the siRNA ULK1 and SKL2001 groups was increased,their invasion and migration abilities were enhanced,the apoptosis rate was decreased,and the protein expression levels of Wnt3α,β-catenin,c-Myc,and Cyclin D1 were upregulated.In the siRNA ULK1+KYA1797K group,the proliferation activity,invasion and migration abilities,apoptosis rate,and protein expression levels of Wnt3α,β-catenin,c-Myc,and Cyclin D1 showed no significant changes compared with those in the the negative control group. Conclusion Inhibition of ULK1 expression increases the proliferation,migration and invasion activities of trophoblastic cells in preeclampsia rats and reduces the apoptosis rate,and these processes are related to the activation of the Wnt/β-catenin pathway.

Key words: unc-51 like kinase 1, Wnt signaling pathway, trophoblastic cell, invasion, migration, apoptosis

中图分类号:

R714.2

丁淑, 苏根娣, 张林艳, 姜文静. 基于Wnt信号通路分析ULK1对子痫前期大鼠胎盘滋养层细胞侵袭、迁移的影响[J]. 医学分子生物学杂志, 2026, 23(4): 412-417.

DING Shu, SU Gendi, ZHANG Linyan, JIANG Wenjing. Effect of ULK1 on Invasion and Migration of Placental Trophoblastic Cells of Preeclampsia Rats via Wnt Signaling Pathway[J]. Journal of Medical Molecular Biology, 2026, 23(4): 412-417.

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