罗哌卡因在小鼠过敏性鼻炎模型中的治疗作用及机制

doi:10.3870/j.issn.1672-8009.2026.04.012

医学分子生物学杂志 ›› 2026, Vol. 23 ›› Issue (4): 452-457.doi: 10.3870/j.issn.1672-8009.2026.04.012

罗哌卡因在小鼠过敏性鼻炎模型中的治疗作用及机制

王欢¹, 张贺², 殷萌³, 张惠男¹, 刘光¹, 郭继峰¹

首都医科大学附属北京儿童医院保定医院 ¹手术麻醉科,²呼吸科河北省保定市,071000
³中国中医科学院广安门医院保定医院麻醉科河北省保定市,071000

收稿日期:2025-12-09 发布日期:2026-09-28
通讯作者: 王欢(E-mail:smkwh666@163.com)

Therapeutic Effect and Mechanism of Ropivacaine in Mice Model of Allergic Rhinitis

WANG Huan¹, ZHANG He², YIN Meng³, ZHANG Huinan¹, LIU Guang¹, GUO Jifeng¹

¹Department of Anesthesiology and Surgical Anesthesiology,²Department of Respiratory Medicine,Baoding Hospital,Beijing Children’s Hospital,Capital Medical University,Baoding,Hebei,071000,China
³Department of Anesthesiology,Guang’anmen Hospital Baoding Hospital,Chinese Academy of Traditional Chinese Medicine,Baoding,Hebei,071000,China

Received:2025-12-09 Published:2026-09-28
Contact: WANG Huan(E-mail:smkwh666@163.com)

摘要/Abstract

摘要： 目的探究罗哌卡因(ropivacaine)缓解卵清蛋白(ovalbumin,OVA)诱导的小鼠过敏性鼻炎(allergic rhinitis,AR)的作用机制。方法小鼠随机分为对照组、AR组、Ropivacaine低剂量组(1 mg/kg)、Ropivacaine高剂量组(5 mg/kg)和地塞米松(Dex)阳性组(2 mg/kg),各5只。末次给药后进行指标评估。结果 Ropivacaine高剂量组和Dex组小鼠体质量高于AR组(P<0.05),相对脾脏质量,搓鼻、打喷嚏和流鼻涕次数,血清组胺、OVA特异性免疫球蛋白E(IgE)、总IgE、总IgG1和β-己糖胺酶水平,鼻灌洗液中IL-1β、IL-6、TNF-α、IL-4和IL-5水平及鼻黏膜组织p65、p-IkBα蛋白表达均低于AR组(P<0.05),嗜酸性粒细胞浸润减少。结论高剂量罗哌卡因可缓解OVA诱导的AR小鼠鼻炎症状及鼻黏膜炎性浸润,其机制可能与抑制NF-κB信号通路有关。

关键词: 罗哌卡因, 卵清蛋白, 过敏性鼻炎, 细胞因子, NF-κB信号通路

Abstract: Objective To explore the effect of ropivacaine on alleviating ovalbumin(OVA)-induced allergic rhinitis(AR)in mice. Methods Mice were randomly divided into 5 groups:control group,AR group,low-dose Ropivacaine group(1 mg/kg),high-dose Ropivacaine group(5 mg/kg)and Dexamethasone(Dex)positive group(2 mg/kg),with 5 mice in each group.Indicator evaluations were conducted after the last drug administration. Results The body weight of mice in the high-dose Ropivacaine group and Dex group was greater than that in the AR group(P<0.05),and the relative spleen weight,the times of nose rubbing,sneezing and runny nose,levels of serum histamine,OVA-specific immunoglobulin E(IgE),total IgE,total IgG1 and β-hexosaminidase,levels of iIL-1β,IL-6,TNF-α,IL-4 and IL-5 in nasal lavage fluid and the protein expression levels of p65 and p-IkBα in the nasal mucosa were lower than those in the AR group(P<0.05).Eosinophil infiltration was reduced. Conclusion High-dose ropivacaine can relieve OVA-induced rhinitis symptoms and inflammatory infiltration of nasal mucosa of mice with AR.The mechanism may be related to the inhibition of NF-κB signaling pathway.

Key words: ropivacaine, ovalbumin, allergic rhinitis, cytokines, NF-κB signaling pathway

中图分类号:

R962.1

王欢, 张贺, 殷萌, 张惠男, 刘光, 郭继峰. 罗哌卡因在小鼠过敏性鼻炎模型中的治疗作用及机制[J]. 医学分子生物学杂志, 2026, 23(4): 452-457.

WANG Huan, ZHANG He, YIN Meng, ZHANG Huinan, LIU Guang, GUO Jifeng. Therapeutic Effect and Mechanism of Ropivacaine in Mice Model of Allergic Rhinitis[J]. Journal of Medical Molecular Biology, 2026, 23(4): 452-457.

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罗哌卡因在小鼠过敏性鼻炎模型中的治疗作用及机制

Therapeutic Effect and Mechanism of Ropivacaine in Mice Model of Allergic Rhinitis

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