miR-330-3p/LHX6轴对子宫内膜癌细胞增殖、迁移和侵袭的影响

doi:10.3870/j.issn.1672-8009.2026.02.011

医学分子生物学杂志 ›› 2026, Vol. 23 ›› Issue (2): 186-193.doi: 10.3870/j.issn.1672-8009.2026.02.011

miR-330-3p/LHX6轴对子宫内膜癌细胞增殖、迁移和侵袭的影响

乔美玲¹, 张莉华², 赵晓阳³, 卫雷⁴

¹邯郸市妇幼保健院检验一科河北省邯郸市,056001
²邯郸市中心医院妇二科河北省邯郸市,056001

收稿日期:2025-10-20 出版日期:2026-03-31 发布日期:2026-04-03
通讯作者: 乔美玲(E-mail:M15100457998@126.com)
基金资助:
河北省医学科学研究课题项目(No.20241717)

Effect of miR-330-3p/LHX6 Axis on Proliferation,Migration and Invasion of Endometrial Cancer Cells

QIAO Meiling¹, ZHANG Lihua², ZHAO Xiaoyang³, WEI Lei⁴

¹Department of Laboratory Medicine,Maternity and Child Health Hospital,Handan,Hebei,056001,China
²Department of Obstetrics and Gynecology,Handan Central Hospital,Handan,Hebei,056001,China

Received:2025-10-20 Online:2026-03-31 Published:2026-04-03
Contact: QIAO Meiling(E-mail:M15100457998@126.com)
Supported by:
Medical Science Research Project of Hebei Province(No.20241717)

摘要/Abstract

摘要： 目的探讨miR-330-3p靶向LIM同源框6(LIM homeobox 6,LHX6)对子宫内膜癌生物学行为的影响。方法 TCGA数据库分析miR-330-3p、LHX6在子宫内膜癌中的临床意义,Starbase预测miR-330-3p、LHX6结合位点并验证。构建miR-330-3p过表达、miR-330-3p/LHX6双过表达HEC108、Ishikawa细胞系,观察细胞增殖、迁移和侵袭能力,建立miR-330-3p过表达HEC108细胞裸鼠移植瘤,观察移植瘤生长情况。结果子宫内膜癌组织miR-330-3p表达水平升高,LHX6表达水平降低,miR-330-3p靶向抑制LHX6表达。miR-330-3p过表达可促进HEC108、Ishikawa细胞增殖、迁移和侵袭和移植瘤生长,miR-330-3p过表达基础上LHX6表达上调可部分逆转miR-330-3p的作用效果。结论 miR-330-3p可靶向抑制LHX6表达、增强子宫内膜癌恶性生物学行为。

关键词: 子宫内膜癌, miR-330-3p, LIM同源框6

Abstract: Objective To explore the effect of miR-330-3p on proliferation,migration,and invasion of endometrial cancer by targeting LIM homeobox 6(LHX6).Methods The clinical significance of miR-330-3p and LHX6 in endometrial cancer was analyzed by TCGA database.The binding site between miR-330-3p and LHX6 was predicted by Starbase and was then validated.HEC108 and Ishikawa cell lines with miR-330-3p overexpression or miR-330-3p/LHX6 overexpression were constructed to observe cells proliferation,migration and invasion.The xenograft nude mice model with miR-330-3p overexpressed HEC108 cells were constructed to observe the growth of transplanted tumors.Results The expression level of miR-330-3p was increased,while the expression level of LHX6 was decreased in endometrial cancer tissues,and miR-330-3p could inhibit the expression of LHX6.Overexpression of miR-330-3p could promote the proliferation,migration and invasion of HEC108 and Ishikawa cells and the growth of transplanted tumors.Overexpression of LHX6 could partially reverse the effect of miR-330-3p overexpression in cells.Conclusion miR-330-3p can promote proliferation,migration and invasion of endometrial cancer by inhibiting the expression of LHX6.

Key words: endometrial cancer, microRNA-330-3p, LIM homeobox 6

中图分类号:

R737.33

乔美玲, 张莉华, 赵晓阳, 卫雷. miR-330-3p/LHX6轴对子宫内膜癌细胞增殖、迁移和侵袭的影响[J]. 医学分子生物学杂志, 2026, 23(2): 186-193.

QIAO Meiling, ZHANG Lihua, ZHAO Xiaoyang, WEI Lei. Effect of miR-330-3p/LHX6 Axis on Proliferation,Migration and Invasion of Endometrial Cancer Cells[J]. Journal of Medical Molecular Biology, 2026, 23(2): 186-193.

参考文献

[1] 乔美玲,张莉华.脂联素对子宫内膜癌细胞增殖、侵袭及PI3K/AKT/NF-κB通路的影响[J].医学分子生物学杂志,2025,22(5):488-493.
QIAO M L,ZHANG L H.Effect of adiponectin on endometrial cancer cell growth,invasion,and PI3K/AKT/NF-κB pathway[J].J Med Mol Biol,2025,22(5):488-493.
[2] 舒中献,詹红星,王越,等.外周血NLR、VEGF、miRNA let-7a水平与喉癌患者淋巴结转移的相关性[J].分子诊断与治疗杂志,2023,15(4):647-650.
SHU Z X,ZHAN H X,WANG Y,et al.Correlation between expression levels of peripheral blood NLR,VEGF and miRNA let-7 a and lymph node metastasis in patients with laryngeal cancer[J].J Mol Diagn Ther,2023,15(4):647-650.
[3] WEI C H,WU G,CAI Q,et al.microRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway[J].J Hematol Oncol,2017,10(1):125.
[4] ZHANG Y,GUO C,YANG S,et al.NONHSAT021545/miR-330-3p/EREG:a cooperative axis in breast cancer prognosis and treatment[J].J Clin Med,2023,12(7):2478.
[5] LI W,WANG L,WU Y,et al.Weighted gene co-expression network analysis to identify key modules and hub genes associated with atrial fibrillation[J].Int J Mol Med,2020,45(2):401-416.
[6] LIU H,FANG Y,HOU B,et al.CircPACRGL promoted cell proliferation,migration and invasion as well as inhibited cell apoptosis in colorectal cancer via regulation of the miR-330-3p/CNBP axis[J].Mol Cell Biochem,2023,478(7):1633-1644.
[7] 鲁之中,李锡凌,李凯,等.miR-27 a-3 p及YAP1 mRNA在乳腺癌中的表达及其与临床病理、生存预后的关系分析[J].四川大学学报(医学版),2025,56(2):521-527.
LU Z Z,LI X L,LI K,et al.Expressions of miR-27a-3p mRNA and YAP1 mRNA in breast cancer and the relationship with clinicopathology and survival prognosis[J].J Sichuan Univ(Med Sci),2025,56(2):521-527.
[8] 黄劲熹,唐昊,胡强.前列腺癌患者血清miR-425-5p、miR-221变化及与术后复发转移的关系分析[J].分子诊断与治疗杂志,2025,17(10):1896-1899.
HUANG J X,TANG H,HU Q.Changes of serum miR-425-5p and miR-221 in patients with prostate cancer and relation-ship with postoperative recurrence/metastasis[J].J Mol Diagn Ther,2025,17(10):1896-1899.
[9] 白咪红,张洁,寇莉,等.miR-425-5p在子宫内膜癌中的诊断价值及其对子宫内膜癌细胞增殖、迁移和凋亡的影响[J].医学分子生物学杂志,2025,22(2):173-179.
BAI M H,ZHANG J,KOU L,et al.Diagnostic value of miR-425-5p in endometrial cancer and effect of inhibiting miR-425-5p on proliferation,migration and apoptosis in endometrial cancer cells[J].J Med Mol Biol,2025,22(2):173-179.
[10] TANG M,WANG F,WANG K,et al.Circ_0058063 promotes progression of thyroid cancer by sponging miR-330-3p/SDC4 axis[J].Anticancer Drugs,2022,33(7):642-651.
[11] BASIRI P,AFSHAR S,AMINI R,et al.Evaluation of miR-330-3p and BMI1 expression in colorectal cancer patients,healthy adjacent tissues,and polypoid adenomatous lesions[J].Int J Mol Cell Med,2022,11(4):334-345.
[12] WANG J,KE S,GONG Y,et al.Circ_0011385 knockdown inhibits cell proliferation,migration and invasion,whereas promotes cell apoptosis by regulating miR-330-3p/MYO6 axis in colorectal cancer[J].Biomed J,2023,46(1):110-121.
[13] 叶芷杉,农雪萍,王艳云,等.外泌体来源的circRNA_051778在肺腺癌性恶性胸腔积液和结核性胸腔积液中的表达及作用研究[J].四川大学学报(医学版),2024,55(5):1254-1263.
YE Z S,NONG X P,WANG Y Y,et al.Expression of circRNA_051778 in lung adenocarcinoma-associated malignant and tuberculous pleural effusions and its clinical significance[J].J Sichuan Univ(Med Sci),2024,55(5):1254-1263.
[14] CHEN Y,LIU H,ZOU J,et al.Exosomal circ_0091741 promotes gastric cancer cell autophagy and chemoresistance via the miR-330-3p/TRIM14/Dvl2/Wnt/β-catenin axis[J].Hum Cell,2023,36(1):258-275.
[15] FALTINGS L J,LIN F,ZAHRAN M,et al.SOX2 commands LIM homeobox transcription factors in choroid plexus development and tumorigenesis[J].Neuro Oncol,2025,27(8):2006-2022.
[16] WANG L,ZHOU Y,CAO C,et al.The exon 12-containing LHX6 isoforms promote cervical cancer cell proliferation by regulating the MAPK signaling pathway[J].Cancer Med,2022,11(19):3657-3673.
[17] JESSA S,DE COLA A,CHANDARANA B,et al.FOXR2 targets LHX6+/DLX+ neural lineages to drive central nervous system neuroblastoma[J].Cancer Res,2025,85(2):231-250.
[18] 张明谦,邓虹,李艳,等.LHX6基因抑制肺腺癌的转移机制[J].实用医学杂志,2019,35(22):3447-3451,3456.
ZHANG M Q,DENG H,LI Y,et al.Mechanism of LHX6 gene in inhibiting metastasis of lung adenocarcinoma[J].Jo Prac Med,2019,35(22):3447-3451,3456.
[19] XU Y,HU J,LV Q,et al.Endometrium-derived mesenchymal stem cells suppress progression of endometrial cancer via the DKK1-Wnt/β-catenin signaling pathway[J].Stem Cell Res Ther,2023,14(1):159.
[20] CHEN R,LIU J,HU J,et al.DLGAP5 knockdown inactivates the Wnt/β-catenin signal to repress endometrial cancer cell malignant activities[J].Environ Toxicol,2023,38(3):685-693.

miR-330-3p/LHX6轴对子宫内膜癌细胞增殖、迁移和侵袭的影响

Effect of miR-330-3p/LHX6 Axis on Proliferation,Migration and Invasion of Endometrial Cancer Cells

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