关键词: miR-181a-3p, 细胞因子信号传导抑制因子5, 急性淋巴细胞白血病, 增殖, 迁移, 侵袭, 上皮间质转化

Abstract: Objective To investigate the effect of microRNA(miRNA)-181 a-3 p on the malignant progression of acute lymphoblastic leukemia(ALL)cells by targeting suppressor of cytokine signaling 5(SOCS5).Methods The expression levels of miR-181a-3p and SOCS5 in ALL cells were detected by RT-qPCR and Western blotting.MoIt4 cells were divided into 6 groups:anti-miR-NC group,anti-miR-181a-3p group,pcDNA group,PCDNA-SOCS5 group,anti-miR-181a-3p+si-NC group and anti-miR-181a-3p+si-SOCS5 group.RT-qPCR was used to detect the expression levels of miR-181a-3p and SOCS5.CCK8 and Transwell assay were used to detect cell proliferation,migration and invasion.Western blotting was used to detect the expression of SOCS5,CDK2,N-cadherin,E-cadherin and Vimentin.Dual luciferase gene reporter assay was used to detect the targeting relationship between miR-181a-3p and SOCS5.Results Compared with that in the PBMCS cells,the expression level of miR-181a-3p in the MoIt4 and Jurkat cells was increased(P<0.05),while the expression levels of SOCS5 mRNA and protein were decreased(P<0.05).Inhibition of miR-181a-3p or overexpression of SOCS5 decreased the proliferative activity,migration and invasion of MoIt4 cells,and the expression levels of N-cadherin,CDK2 and Vimentin,and increased the expression level of E-cadherin(P<0.05).miR-181a-3p negatively targeted SOCS5,inhibition of SOCS5 reversed the effect of miR-181a-3p on the proliferation,migration,invasion and epithelial-to-mesenchymal transition(EMT)in MoIt4 cells.Conclusion miR-181a-3p targets SOCS5 and negatively regulates the proliferation,migration,invasion and EMT of ALL cells.

Key words: miR-181a-3p, SOCS5, Acute lymphoblastic leukemia, Proliferation, Migration, Invade, Epithelial-to-mesenchymal transition