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Abstract: Objective To investigate the effect of blocking PI3K / Akt / mTOR signaling on leukemia cells. Methods Acute myeloid leukemia cell line HL-60 was selected, cells were randomly divided into 4 groups: blank control group, low concentration group, medium concentration group and high concentration group. Cells in the blank control group was given normal saline, cells in the low concentration group, medium concentration group and high concentration group were treated with 10, 25 and 50 Pmol / L PI3K / Akt / mTOR signaling blocker GDC-0349. CCK-8 method was used to detect the cell proliferation. Cell apoptosis was detected by flow cytometry, and the expression level of PI3K / Akt / mTOR and apoptosis related proteins were detected by Western blotting. Results Compared with those in the low and medium concentration groups, the absorbance ratios (A values) of cells in the high concentration group at 24, 48 and 72 h time-points were significantly lower (P < 0. 05). The apoptosis rates of cells in the low, medium and high concentration groups were significantly higher than that of cells in the blank control group ( P < 0. 05), and theapoptosis rate of cells in the high concentration group was significantly lower than those of cells inthe low and medium concentration groups (P < 0. 05). The relative expression levels of P110, Aktand mTOR in the high concentration group were significantly lower than those in the blank controlgroup, low and medium concentration groups (P < 0. 05). The relative expression levels of Bcl2 andCaspase3 proteins in the low, medium and high concentration groups were significantly lower thanthat in the blank control group (P < 0. 05). The relative expression levels of Bcl2 and Caspase3 in the high concentration group were significantly lower than those in other groups (P < 0. 05). Conclusion Blocking PI3K / Akt / mTOR signaling can inhibit the proliferation and promote the apoptosis of HL-60 cells.

Key words: PI3K / Akt / mTOR signal, leukemia, proliferation, apoptosis