医学分子生物学杂志 ›› 2023, Vol. 20 ›› Issue (4): 305-309.doi: 10.3870/j.issn.1672-8009.2023.04.005

• 论著 • 上一篇    下一篇

血清 miR-181a 水平在 COPD 患者中的表达水平及临床意义

  

  1. 南京脑科医院胸科院区呼吸科 南京市, 210029
  • 出版日期:2023-07-31 发布日期:2023-09-06
  • 基金资助:
    江苏省卫生健康委科研项目 (No. H2019040)

Expression of Serum miR-181a in COPD Patients and Its Clinical Significanc

  1. Department of Respiratory, Thoracic Branch of Nanjing Brain Hospital, Nanjing, 210029, China
  • Online:2023-07-31 Published:2023-09-06

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摘要： 目的 探讨血清微小 RNA-181a (miR-181a) 在慢性阻塞性肺疾病 ( chronic obstructive pulmonary disease, COPD) 患者中的表达水平及临床意义。 方法 选取南京脑科医院于 2019 年 6 月至 2021 年 6 月呼 吸科收治的诊断为 COPD 的患者 80 例, 根据病情分为 2 组: COPD 组 (稳定期 38 例) 和 AECOPD 组 (急 性加重期 42 例), 其中 AECOPD 组按照预后再分为预后良好组 (18 例) 与预后不良组 (24 例)。 另选取健 康体检者 40 例作为对照组。 采用 qRT-PCR 法检测血清 miR-181a 表达水平, 使用肺功能仪检测研究对象的 肺功能。 采用 Pearson 相关性分析血清 miR-181a 水平与肺功能的相关性。 应用 COX 回归分析影响 AECOPD 患者不良预后的危险因素。 结果 AECOPD 组患者有吸烟史比例、 miR-181a 水平高于 COPD 组和对照组, FEV1 / FVC、 FEV1 % 、 FVC % 低于 COPD 组和对照组。 Pearson 相关性分析结果显示, AECOPD 患者血清 miR-181a 表达水平与 FEV1 / FVC、 FEV1 % 、 FVC % 呈负相关 (P< 0. 05)。 与预后良好组患者比较, 预后 不良组患者血清 miR-181a 水平显著升高 (P< 0. 05)。 miR-181a 水平是影响 AECOPD 患者预后的独立危险 因素 (P < 0. 05)。 结论 血清 miR-181a 表达水平与 COPD 患者的急性期发作、 疾病的进展及预后有关。

关键词: 微小 RNA-181a, COPD, 肺功能 ,

Abstract: Objective To investigate the expression level and clinical significance of serum microRNA-181a ( miR-181a ) in patients with chronic obstructive pulmonary disease ( COPD). Methods Eighty patients diagnosed with COPD who were admitted to the respiratory department in Nanjing Brain Hospital from June 2019 to June 2021 were selected, and they were divided into the COPD group (38 cases, in stable stage) and the AECOPD group (42 cases, in acute exacerbation stage). Patients in the acute exacerbation stage were then divided into the good prognosis group (18 cases) and the poor prognosis group ( 24 cases) according to the prognosis. Another 40 healthy people who came to hospital for check-up were selected as controls. The qRT-PCR method was used to detect the expression level of serum miR-181a, and the pulmonary function test instrument was used to detect the lung function of the subjects. The correlation between the serum level of miR-181a and the lung function was analyzed by Pearson correlation analysis. COX regression analysis was used to analyze the risk factors that affected the poor prognosis of patients with AECOPD. Results The proportion of patients with smoking history and the level of miR-181a in the AECOPD group were higher than those in the COPD group and the control group, the FEV1 / FVC, FEV1 % , and FVC % in the AECOPD group were also lower than those in the COPD group and the control group. The results of Pearson correlation analysis showed that serum miR-181a expression level in AECOPD patients was negatively correlated with FEV1 / FVC, FEV1 % , and FVC % (P< 0. 05). Compared with the serum level of miR-181a in patients in the good prognosis group, tthat in the poor prognosis group was significantly higher (P < 0. 05). miR-181a level was an independent risk factor for the prognosis of AECOPD patients (P< 0. 05). Conclusion The expression level of serum miR-181a is related to the acute onset, disease progression and prognosis of COPD patients.

Key words: microRNA-181a, chronic obstructive pulmonary disease, lung function ,

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