关键词: 宫颈癌, LINC01133, 炎性因子, 超氧化物歧化酶, P65

Abstract: Objective To explore the function and mechanism of interfering long non-coding RNA LINC01133 in cervical cancer cells. Methods TCGA database and qRT-PCR were used to analyze the expression of LINC01133 in cervical cancer tissues and cells. The knockdown efficiency of LINC01133-shRNAs was detected by qRT-PCR. SiHa cells were divided into 3 groups: control group, shRNA-NC group and LINC01133-shRNA1 group. Colony formation assay was used to detect cell proliferation. ELISA assay was used to detect the levels of tumor necrosis factor-α ( TNF-α), interleukin (IL) -6 and IL-1β. The nuclear translocation of P65 in SiHa cells was detected by immunofluorescence. The phosphorylation of P65 was detected by Western blotting. The levels of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were detected by kits. A nude mouse xenograft model was established to analyze the effect of interfering LINC01133 expression on tumor growth. Results The expression of LINC01133 was significantly up-regulated in cervical cancer tissues and cells (P < 0. 05). The knockdown efficiency of LINC01133-shRNA1 was the best, thus the LINC01133-shRNA1 was selected for the subsequent experiments. The colony formation rate, the levels of inflammatory factors, the phosphorylation level of P65, the proportion of P65 level in nuclear, and the LDH level in the LINC01133-shRNA1 group were significantly increased when compared with those in the control group (P< 0. 05), while the SOD activity was significantly decreased ( P < 0. 05 ) . The volume weight of the tumor tissues, and the SOD activity in the LINC01133-shRNA1 group were significantly decreased when compared with those in the shRNA-NC group (P< 0. 05), while the phosphorylation level of P65 was significantly increased (P< 0. 05). Conclusion Interfering with the LINC01133 shRNA inhibits cervical cancer cell growth, decreases inflammatory factor levels and suppresses the oxidative stress in cervical cancer cells.

Key words: cervical cancer, LINC01133, inflammatory factors, superoxide dismutase, P65