关键词: 宫颈癌, 跨膜丝氨酸蛋白酶 4, 线皮素-1, 预后 

Abstract: Objective To investigate the expression of transmembrane protease serine 4 (TMPRSS4) and LAD1 in cervical cancer tissues and their relationship with the prognosis of cervical cancer patients. Methods A total of 79 patients with cervical cancer who underwent surgical treatment in Danyang People’s Hospital from June 2015 to June 2018 were selected as the research objects. The 79 cancerous tissue specimens and 60 para-cancerous tissue specimens were collected from cervical cancer patients during the operation. The immunohistochemical staining method and realtime fluorescent quantitative PCR (qRT-PCR) method were used to detect the protein and mRNA expression levels of TMPRSS4 and LAD1 in tissues. The clinical pathological parameters of the patients were collected and the patients were followed up for 3 years. The relationship between the expression levels of TMPRSS4 and LAD1 and the clinical pathological parameters of patients, as well as the factors that affect the prognosis of patients with cervical cancer were analyzed. The correlation between TMPRSS4 and LAD1 expression was analyzed with Pearson method. The predictive value of TMPRSS4 and LAD1 levels on the prognosis of patients was analyzed with the receiver operating characteristic curve (ROC). Results The expression levels of TMPRSS4 and LAD1 in cervical cancer tissues were higher than those in adjacent tissues (P < 0. 05). The expression levels of TMPRSS4 and LAD1 in cervical cancer tissues were significantly positively correlated (r = 0. 249, P< 0. 05). The expression levels of TMPRSS4 and LAD1 proteins in cervical cancer tissue were related to the FIGO stage and tumor differentiation degree (P < 0. 05). FIGO staging, tumor differentiation, TMPRSS4 and LAD1 protein expression levels were the factors affecting the prognosis of patients (P< 0. 05). The expression levels of TMPRSS4 and LAD1 in the cancer tissues of patients in survival group were significantly lower than those in death group ( P < 0. 05 ). The ROC results showed that the areas under the curve (AUCs) for TMPRSS4 and LAD1 to assess the prognosis of patients were 0. 756 and 0. 909, respectively, the corresponding sensitivities of the assessment were 91. 11 % and 52. 94 % respectively, and the specificities were 85. 29 % and 85. 29 % respectively. The AUC for the combination of using TMPRSS4 and LAD1 to assess the prognosis of patients was 0. 952, the sensitivity of the assessment was 88. 89 % , and the specificity was 88. 24 % . Conclusion TMPRSS4 and LAD1 are both highly expressed in cervical cancer tissues. The expression levels of TMPRSS4 and LAD1 are significantly positively correlated, and they can be used for the prognosis of cervical cancer patients.

Key words: cervical cancer, transmembrane protease serine 4, ladinin-1, prognosis