CaMKⅡ在醛固酮瘤中表达及其临床意义*

doi:10.3870/j.issn.1672-0741.25.09.018

华中科技大学学报（医学版） ›› 2026, Vol. 55 ›› Issue (2): 244-250.doi: 10.3870/j.issn.1672-0741.25.09.018

CaMKⅡ在醛固酮瘤中表达及其临床意义^*

耿小晶^1,2, 曾幸¹, 林莹², 曾庆敏¹, 黄茵^1△, 张少玲^2△

¹中山大学附属第五医院凤凰国际医学中心,珠海 519000
²中山大学附属孙逸仙纪念医院内分泌科,广州 510120

出版日期:2026-04-15 发布日期:2026-04-16
通讯作者: ^△E-mail:Huangyin3@sysu.edu.cn(黄茵);zhangshaoling88@hotmail.com(张少玲)
作者简介:耿小晶,女,1981年生,主治医师,E-mail:gengxj3@163.com
基金资助:
^*国家自然科学基金资助项目( No.81471011,No.81600606)

Expression of CaMKⅡ in Aldosterone-producing Tumors and Its Clinical Significance

Geng Xiaojing^1,2, Zeng Xing¹, Lin Ying² et al

¹Phoenix International Medical Center,the Fifth Affiliated Hospital of Sun Yat-sen University,Zhuhai 519000,China
²Sun Yat-sen Memorial Hospital of Sun Yat-sen University,Guangzhou 510120

Online:2026-04-15 Published:2026-04-16
Contact: ^△E-mail:Huangyin3@sysu.edu.cn;zhangshaoling88@hotmail.com

摘要/Abstract

摘要： 目的探讨钙/钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)在醛固酮瘤(APA)组织、肾上腺无功能腺瘤(NFA)组织、正常肾上腺组织的表达特征。方法定量PCR(qRT-PCR)、Western blot和免疫组织化学方法检测APA、NFA和正常肾上腺中CaMKⅡ基因和蛋白表达情况。结果 qRT-PCR和Western blot检测显示,APA组织中CaMKⅡ mRNA和蛋白表达水平明显高于正常肾上腺和NFA(均P<0.05)。免疫组织化学检测显示,CaMKⅡ在正常肾上腺和APA瘤旁组织球状带的表达呈强阳性,在束状带和网状带的表达呈弱阳性,CaMKⅡ主要定位于胞质;正常肾上腺组织中,CaMKⅡ在球状带呈不连续性表达,而在APA瘤旁组织球状带中呈连续性表达;CaMKⅡ在APA沿血管和被膜下分布,且均定位于胞质。CaMKⅡ 免疫组织化学染色的免疫反应积分(IRS)在APA高于正常肾上腺和NFA。与正常肾上腺和NFA瘤旁组织相比,APA瘤旁组织重塑显著增强(均P<0.05)。CaMKⅡ IRS×体积值与卧位血浆醛固酮(PAC)、立位PAC、24 h尿醛固酮呈正相关,而与卧位血浆肾素(PRA)、立位PRA及血钾均无明显相关。结论 CaMKⅡ可能调控病理性醛固酮合成与分泌,参与了肾上腺皮质的重塑。

关键词: CaMKⅡ, 醛固酮瘤, 醛固酮合成

Abstract: Objective To investigate the expression of CaMKⅡ in aldosterone-producing adenoma(APA),adrenal nonfunctional adenoma(NFA),and normal adrenal tissue. Methods The mRNA and protein expression of CaMKⅡ in APA,NFA,and normal adrenal tissues was detected by quantitative PCR(qRT-PCR),Western blotting,and immunohistochemical staining. Results Both qRT-PCR and Western blot analysis revealed that the mRNA and protein levels of CaMKⅡ were significantly higher in APA than in normal adrenal glands and NFA(all P<0.05).Immunohistochemical staining in the adrenal cortex demonstrated that CaMKⅡ was primarily detected in the zona glomerulosa(ZG),with weak or negligible expression in the zona fasciculata and reticularis.In APA tissues,CaMKⅡ immunoreactivity was observed in a subpopulation of steroidogenic cells resembling ZG-like cells,particularly located beneath the capsule or surrounding blood vessels.The peritumoral tissue adjacent to APA exhibited significantly more remodeling than that adjacent to NFA and normal adrenal tissue(both P<0.05).Furthermore,CaMKⅡ immunoreactivity score(IRS)was significantly higher in APA tissues than both normal adrenal and NFA tissues(both P<0.05).A positive correlation was found between CaMKⅡ IRS multiplied by tumor volume and supine plasma aldosterone concentration(PAC)(r=0.41,P<0.05),standing PAC(r=0.47,P<0.05),and 24 h urinary aldosterone(r=0.38,P<0.05).No significant correlations were observed with supine plasma renin activity(PRA),orthostatic PRA,or serum K⁺levels. Conclusion CaMKⅡ may be involved in pathological aldosterone synthesis and secretion,as well as adrenal cortex remodeling.

Key words: CaMKⅡ, aldosterone-producing adenoma, aldosterone biosynthesis

中图分类号:

R586.24

耿小晶, 曾幸, 林莹, 曾庆敏, 黄茵, 张少玲. CaMKⅡ在醛固酮瘤中表达及其临床意义^*[J]. 华中科技大学学报（医学版）, 2026, 55(2): 244-250.

Geng Xiaojing, Zeng Xing, Lin Ying et al. Expression of CaMKⅡ in Aldosterone-producing Tumors and Its Clinical Significance[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(2): 244-250.

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CaMKⅡ在醛固酮瘤中表达及其临床意义^*

Expression of CaMKⅡ in Aldosterone-producing Tumors and Its Clinical Significance

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