Effect of miR-370-3p on Migration and Invasion of Hepatocellular Carcinoma Cells HepG2 via Targeting STAT3

doi:10.3870/j.issn.1672-8009.2026.04.002

Abstract

Abstract: Objective To explore the influence of miR-370-3p on migration and invasion of hepatocellular carcinoma HepG2 cells by targeting STAT3. Methods The miR-NC or miR-370-3p mimic and pcDNA or pcDNA-STAT3 were transfected into HepG2 cells,and the mRNA relative expression levels of miR-370-3p and STAT3 were detected.miR-370-3p mimic and STAT3-wild type(wt)or STAT3-mutant(mut)were co-transfected into HepG2 cells.Finally,the control group,miR-370-3p group,STAT3 group and miR-370-3p+STAT3 group were established.Transwell assay and Wound-healing assay were applied to detect the invasion,migration of cells.Western blot assay was applied to detect the expression level of epithelial-mesenchymal transition associated proteins.The orthotopic xenograft mouse model was established,and mice were divided into miR-NC group and miR-370-3p group. Results Compared with the control group,the relative expression level of miR-370-3p was significantly upregulated in the miR-370-3p mimic group.In the pcDNA-STAT3 group,the relative expression level of STAT3 mRNA was significantly upregulated.Overexpression of miR-370-3p inhibits invasion,migration,and affect the expression of epithelial-mesenchymal transition associated pproteins in HepG2 liver cancer cells.Additionally,miR-370-3p targets STAT3 to suppress the aforementioned metastatic capabilities of HepG2 liver cancer cells.In in vivo experiments,overexpression of miR-370-3p inhibits tumor growth. Conclusion miR-370-3p can inhibit the invasion,migration of hepatocellular carcinoma cells HepG2 by targeting STAT3.

Key words: miR-370-3p, STAT3, hepatocellular carcinoma, invasion, migration, epithelial-mesenchymal transition

CLC Number:

R735.7

LIU Yajing, JIA Shichan, YAO Shupeng. Effect of miR-370-3p on Migration and Invasion of Hepatocellular Carcinoma Cells HepG2 via Targeting STAT3[J]. Journal of Medical Molecular Biology, 2026, 23(4): 382-388.

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