Effect of Platycodon grandiflorum Polysaccharides on Oxidative Stress,TLR/NF-κB and JAK2/STAT3 Signaling Pathways in Rats with Ulcerative Colitis

doi:10.3870/j.issn.1672-8009.2026.02.007

Abstract

Abstract: Objective To explore the effect of Platycodon grandiflorum polysaccharides on oxidative stress,and expression of Toll-like receptor(TLR)/nuclear transcription factor kappa B(NF-κB)and Janus kinase 2(JAK2)/signal transduction and activator of transcription 3(STAT3)signaling pathways-related factors in rats with ulcerative colitis(UC).Methods The UC rat model was constructed by dextran sulfate sodium(DSS),then UC rats were treated with different doses of Platycodon grandiflorum polysaccharides for 2 weeks.The disease activity index,pathological changes of colon,apoptosis,levels of inflammatory and oxidative stress factors,and expression levels of TLR4/NF-κB and JAK2/STAT3 related mRNA and proteins were observed.Results After treatment with Platycodon grandiflorum polysaccharides,the disease activity index,apoptosis rate of colon tissues,levels of IL-6,TNF-α,IL-17 and MDA were decreased,levels of IL-10,SOD and GSH-px were increased,expression levels of TLR4,NF-κB,JAK2,STAT3,and p-JAK2 and p-STAT3 in colon tissues were decreased,with a dose dependent manner(P<0.05).Conclusion Platycodon grandiflorum polysaccharides can alleviate colon injury and oxidative stress in colon tissues in UC rats,and inhibit the activation of TLR/NF-κB and JAK2/STAT3 pathways.

Key words: ulcerative colitis, platycodon grandiflorum polysaccharides, oxidative stress, TLR/NF-κB signaling pathway, JAK2/STAT3 signaling pathway

CLC Number:

R285.5

LI Sen, CHANG Jian, LIU Qing’e, YUAN Yunfei, YAN Lingxin. Effect of Platycodon grandiflorum Polysaccharides on Oxidative Stress,TLR/NF-κB and JAK2/STAT3 Signaling Pathways in Rats with Ulcerative Colitis[J]. Journal of Medical Molecular Biology, 2026, 23(2): 156-163.

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