Effect of LncRNA SNHG14 on IL-1β-Induced Chondrocyte Injury by Targeting miR-181a-5p/PRDX3 Axis

doi:10.3870/j.issn.1672-8009.2026.02.002

Abstract

Abstract: Objective To explore the effect of long non coding RNA small nucleolar RNA host gene 14(lncRNA SNHG14)on interleukin(IL)-1β-induced chondrocyte injury by targeting the microRNA-181a-5p(miR-181a-5p)/peroxiredoxin3 (PRDX3) axis.Methods The targeting relationships among lncRNA SNHG14,miR-181a-5p,and PRDX3 were detected using dual-luciferase reporter assay.Human chondrocytes C28/I2 were used to set up the following 10 groups:NC group,IL-1β group,sh-NC group,sh-SNHG14 group,sh-SNHG14+anti-NC group,sh-SNHG14+anti-miR-181a-5p group,miR-NC group,miR-181a-5p mimic group,miR-181a-5p mimic+pcDNA-NC group,miR-181a-5p mimic+pcDNA-PRDX3 group.Cell proliferation and apoptosis were assessed using colony formation assay and flow cytometry,respectively.The levels of IL-6,tumor necrosis factor-alpha(TNF-α),and monocyte chemoattractant protein 1(MCP-1)were measured by enzyme-linked immunosorbent assay.Nitric oxide(NO)level was detected using Griess reagent.Western blotting was performed to determine the expression levels of PRDX3,proliferating cell nuclear antigen(PCNA),Cyclin D1,B-cell lymphoma 2(Bcl-2),and Bcl-2-associated X protein(Bax).Results Dual luciferase assay indicated the targeting relationships between miR-181a-5p and both lncRNA SNHG14 and PRDX3(P<0.05).Compared with the NC group,the IL-1β group showed significantly increased apoptosis rate,IL-6,TNF-α,MCP-1,and NO levels,as well as elevated expression levels of lncRNA SNHG14,PRDX3,and Bax protein,and decreased miR-181a-5p expression,colony formation number,and protein levels of PCNA,Cyclin D1,and Bcl-2(P<0.05).Compared with the sh-NC group or miR-NC group,the sh-SNHG14 group or miR-181a-5p mimic group exhibited significantly higher miR-181a-5p expression level,colony formation number,and protein expression level of PCNA,Cyclin D1,and Bcl-2,along with notably reduced apoptosis rate,IL-6,TNF-α,MCP-1,and NO levels,as well as decreased expression levels of lncRNA SNHG14,PRDX3,and Bax protein(P<0.05).In contrast to the sh-SNHG14+anti-NC group,the sh-SNHG14+anti-miR-181a-5p group demonstrated opposite trends in all above detected indicators(P<0.05).Similarly,all trends of the above index in the miR-181a-5p mimic+pcDNA-PRDX3 group were reversed compared with those in the miR-181a-5p mimic+pcDNA-NC group(P<0.05).Conclusion LncRNA SNHG14 alleviates IL-1β-induced chondrocyte injury by targeting the miR-181a-5p/PRDX3 axis.

Key words: small nucleolar RNA host gene 14, microRNA-181a-5p, peroxiredoxin 3, osteoarthritis

CLC Number:

R349.7

SONG Kailin, LUO Yanning, FANG Hongyu. Effect of LncRNA SNHG14 on IL-1β-Induced Chondrocyte Injury by Targeting miR-181a-5p/PRDX3 Axis[J]. Journal of Medical Molecular Biology, 2026, 23(2): 116-126.

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