NOP14 Overexpression Inhibits NRIP1 Expression and Proliferation and Migration of Ovarian Cancer SKOV3 Cells

doi:10.3870/j.issn.1672-8009.2026.01.009

Abstract

Abstract: Objective To investigate the effect of nucleolar protein 14(NOP14)overexpressi on proliferation and migration of ovarian cancer cells SKOV3 and the underlying mechanism. Methods The expression level of NOP14 in ovarian cancer cell lines(SKOV3,A2780,HO-8910,OVCAR)was detected by RT-qPCR and Western blotting.SKOV3 cells were transfected with the pcDNA-NOP14 plasmid.Cell proliferation was detected by colony formation assay and 5-Ethynyl-2′-deoxyuridine(EdU)staining.Tumor stem cell characteristics were analyzed via serum-free sphere formation assay.The proportion of CD133-positive(CD133⁺)cells was measured by flow cytometry.The epithelial-mesenchymal transition(EMT)process was evaluated by observing cell morphological change and detecting the EMT marker proteins(E-cadherin,N-cadherin,Vimentin).The expression level of nuclear receptor interacting protein 1(NRIP1),and Wnt/β-catenin pathway proteins were detected by RT-qPCR and Western blotting. Results The expression level of NOP14 was significantly lower in the ovarian cancer cell lines than that in the normal ovarian epithelial cells.Overexpression of NOP14 significantly inhibited the proliferation,stem cell characteristics,and EMT of SKOV3 cells(mesenchymal markers N-cadherin and Vimentin were downregulated,while the epithelial marker E-cadherin was upregulated).Moreover,Overexpression of NOP14 suppressed the expression of NRIP1,Wnt and β-catenin. Conclusion NOP14 can inhibit the proliferation,stem cell characteristics,and EMT process of ovarian cancer SKOV3 cells,and negatively regulate the expression of NRIP1,Wnt,β-catenin.

Key words: nucleolar protein 14, ovarian cancer, nuclear receptor interacting protein 1, cell proliferation, epithelial-mesenchymal transition

CLC Number:

R349.5

LI Lingmiao, LI Ping, HU Xiaoli, WANG Dongmei, WU Zixian. NOP14 Overexpression Inhibits NRIP1 Expression and Proliferation and Migration of Ovarian Cancer SKOV3 Cells[J]. Journal of Medical Molecular Biology, 2026, 23(1): 67-73.

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