Mechanism of Sodium Aescinate in Ameliorating Intestinal Mucosal Barrier Dysfunction in Ulcerative Colitis Rats

doi:10.3870/j.issn.1672-8009.2026.02.003

Abstract

Abstract: Objective To explore the effect of sodium aescinate on intestinal mucosal barrier in rats with ulcerative colitis(UC).Methods A total of 65 SD rats were divided into 6 groups:sham operation group,model group,low-dose,medium-dose and high-dose sodium aescinate groups(2.5,5,10 mg/kg),and positive control group(mesalazine,360 mg/kg),10 rats in each group.UC rat models were induced by DSS.Disease activity index(DAI)scores were recorded after drug intervention.The levels of serum D-lactic acid(D-LA),diamine oxidase(DAO),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6 were detected.The ulcer area,length and weight of colon,expression levels of myeloperoxidase(MPO),Occludin,zonula occludens-1(ZO-1),nuclear factor-erythroid 2-related factor 2(Nrf-2),antioxidant response element(ARE)and heme oxygenase-1(HO-1)proteins in colon tissues were compared among different groups.HE staining was performed and pathological scores were recorded.Results Medium-dose and high-dose sodium aescinate significantly reduced DAI score and pathological score,alleviated colonic ulcers,reduced colon weight and increased colon length(P<0.05).The levels of serum D-LA,DAO,TNF-α,IL-1β,IL-6,and colonic MPO were significantly reduced,and the expression of Occludin,ZO-1,p-Nrf2/Nrf2,ARE and HO-1 proteins in colonic tissues was upregulated in the medium- and high-dose sodium aescinate groups(P<0.05).Conclusion Sodium aescinate can ameliorate intestinal mucosal barrier dysfunction in DSS-induced UC rats,and the mechanisms may be related to the inflammation and Nrf-2/ARE/HO-1 signaling pathway.

Key words: sodium aescinate, ulcerative colitis, intestinal mucosal barrier, Nrf-2/ARE/HO-1 signaling pathway

CLC Number:

R285

ZHANG Tong, SI Mengyuan, LIN Ying, WANG Dan, SUN Jirui. Mechanism of Sodium Aescinate in Ameliorating Intestinal Mucosal Barrier Dysfunction in Ulcerative Colitis Rats[J]. Journal of Medical Molecular Biology, 2026, 23(2): 127-133.

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