关键词: 灯盏花素, 牙龈卟啉单胞菌脂多糖, 人牙龈成纤维细胞, 细胞损伤

Abstract: Objective To investigate the effect of breviscapine (BVP) on the Porphyromonasgingivalis lipopolysaccharide ( Pg-LPS, hereinafter referred to as LPS) induced injury in humangingival fibroblasts ( HGFs). Methods HGFs cells were cultured in vitro and divided into 5groups: control group, LPS group, LPS + BVP low-, medium- and high-dose groups. CCK-8 method was used to detect cell proliferation. ELISA method was used to detect the levels of inflammatory factors interleukin-1β ( IL-1β), tumor necrosis factor-α ( TNF-α), interleukin-6 ( IL-6 ) and the activity of superoxide dismutase (SOD). Western blotting method was used to detect the expression levels of cyclin D1, cyclin B1 and apoptosis-related B-cell lymphocytoma-2 ( Bcl-2 ), BCL-2-associated X protein (Bax), Caspase-3 proteins in cells. TBA colorimetric method was used to detect the level of malondialdehyde ( MDA) in cells. DCFH-DA method was used to determinethe level of reactive oxygen species ( ROS) in cells. Results The HGFs cell survival rate, thecell migration rate, the protein expression levels of Cyclin B1, Cyclin D1, Bcl-2 and the SOD activity in the LPS group were significantly decreased when compared with those in the control group (P< 0. 05). The apoptosis rate, the protein expression levels of Bax, Caspase-3, the levels of inflammatory factors TNF-α, IL-1β, IL-6, and the levels of MDA and ROS in the LPS group weresignificantly increased when compared with those in the control group (P< 0. 05). The cell survivalrate, the cell migration rate, the protein expression levels of Cyclin B1, Cyclin D1, Bcl-2 and SOD activity in the LPS + BVP low-, medium-and high-dose groups were significantly increased when compared with those in the LPS group, and the apoptosis rate, the protein expression levels of Bax, Caspase-3, the levels of inflammatory factors TNF-α, IL-1β, IL-6, and the levels of MDA and ROS in the LPS + BVP low-, medium- and high-dose groups were significantly decreased whencompared with those in the LPS group (P< 0. 05). Conclusion BVP can reduce LPS-induced HGFs injury through anti-inflammatory and antioxidant mechanism.

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