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Abstract: Objective To explore the protective effect of Oxysophocarpine (OSC) on myocardial ischemia-reperfusion injury in rat and the mechanism. Methods The cardiac function indicators were detected using echocardiography. The myocardial tissue lesions were observed using HE staining. The levels of heart injury markers and serum inflammatory factor were detected using ELISA methods. The oxidative stress markers were detected using corresponding reagent kits. Theexpression levels of related proteins were detected using Western bloting. Results Compared withthe sham group, the I / R group showed obvious myocardial injury, reduced cardiac function, and up-regulated levels of CK-Mb, Mb and cTnI. The serum level of SOD was significantly decreased, while that of MDA and LDH were increased. The levels of TNF-α, IL-1β, IL-6 and IL-10 were significantly increased, and the expression levels of p-ERK1 / 2 and p-JNK were significantly up-regulated. Compared with the I / R group, OSC treatment significantly improved myocardial injury and myocardial function. OSC treatment significantly inhibited myocardial oxidative stress and the release of pro-inflammatory factors. OSC treatment decreased p-ERK1 / 2 and p-JNK expressions.Conclusion OSC could improve cardiac dysfunction caused by I / R in rats, and inhibit oxidativestress and inflammatory responses. Its mechanism is related to the downregulation of ERK / JNK pathway.

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