活化血小板对内皮细胞凋亡损伤的作用研究

doi:10.3870/j.issn.1672-8009.2026.03.004

医学分子生物学杂志 ›› 2026, Vol. 23 ›› Issue (3): 258-265.doi: 10.3870/j.issn.1672-8009.2026.03.004

活化血小板对内皮细胞凋亡损伤的作用研究

刘红利¹, 李坤¹, 岳雯¹, 位庚¹, 梁俊清^2,3, 田金悦¹

¹石家庄市第二医院中医科石家庄市,050035
²国家中医药管理局重点研究室(心脑血管络病) 石家庄市,050035
³河北省络病重点实验室石家庄市,050035

收稿日期:2025-07-03 发布日期:2026-06-01
通讯作者: 刘红利(E-mail:honglimerry@163.com)
基金资助:
国家重点研发计划“中医药现代化研究”重点专项(No.2017YFC1700500),河北省中医药管理局(No.2022488)

Effect of Platelet Activation on Apoptosis and Damage of Endothelial Cells

LIU Hongli¹, LI Kun¹, YUE Wen¹, WEI Geng¹, LIANG Junqing^2,3, TIAN Jinyue¹

¹Department of Traditional Chinese Medicine,Shijiazhuang Second Hospital,Shijiazhuang,050035,China
²Key Laboratory of State Administration of Traditional Chinese Medicine(Cardiovascular and Cerebrovascular Collateral Diseases),Shijiazhuang,050035,China
³Hebei Province Key Laboratory of Collateral Diseases,Shijiazhuang,050035,China

Received:2025-07-03 Published:2026-06-01
Contact: LIU Hongli(E-mail:honglimerry@163.com)
Supported by:
National Key R&D Program’s Key Project on“Research on the Modernization of Traditional Chinese Medicine”(No.2017YFC1700500),Hebei Provincial Administration of Traditional Chinese Medicine(No.2022488)

摘要/Abstract

摘要： 目的探讨血小板活化对内皮细胞凋亡的影响。方法采集健康人静脉血制备静息血小板悬液和活化血小板悬液,体外培养内皮细胞,建立血小板与内皮细胞共培养模型。采用活细胞成像技术动态观察血小板活化后聚集、粘附、释放过程与内皮细胞凋亡改变过程,同时检测内皮细胞生存活性、线粒体膜电位变化。结果血小板活化后发生明显的聚集反应。与静息血小板相比,血小板活化后,血小板彼此聚集并向内皮细胞移动、粘附。活细胞成像系统观察到活化血小板可引起内皮细胞核浓染、固缩和边缘化,细胞核体积变小,核碎裂,出现凋亡小体。与静息血小板组比较,活化血小板组内皮细胞生存活性和线粒体膜电位降低。结论血小板活化可诱导内皮细胞凋亡损伤。

关键词: 血小板, 内皮细胞, 凋亡, 活细胞成像系统

Abstract: Objective To investigate the effect of platelet activation on the apoptosis of endothelial cells. Methods Platelets were prepared from venous blood,obtained from healthy subjects.Resting platelet suspension,activating platelet suspension,and endothelial cells were cultured in vitro to establish the co-culture model of platelets and endothelial cells.The aggregation,adhesion and release process of platelets after activation and the apoptosis process of endothelial cells were observed by live-cell imaging technique.The endothelial cell survival activity and mitochondrial membrane potential were then detected by kits. Results Activated platelets exhibited significant aggregation,adhesion,and release responses.Compared to resting platelets,activated platelets aggregated,migrated toward endothelial cells,and adhered to them.Live-cell imaging revealed that activated platelets induced nuclear pyknosis,condensation,marginalization,reduced nuclear volume,fragmentation,and the formation of apoptotic bodies in endothelial cells.Compared to the resting platelet group,the activated platelet group showed reduced endothelial cell viability and mitochondrial membrane potential. Conclusion Platelet activation induces apoptotic injury in endothelial cells.

Key words: platelets, endothelial cells, apoptosis, live-cell imaging system

中图分类号:

R543

刘红利, 李坤, 岳雯, 位庚, 梁俊清, 田金悦. 活化血小板对内皮细胞凋亡损伤的作用研究[J]. 医学分子生物学杂志, 2026, 23(3): 258-265.

LIU Hongli, LI Kun, YUE Wen, WEI Geng, LIANG Junqing, TIAN Jinyue. Effect of Platelet Activation on Apoptosis and Damage of Endothelial Cells[J]. Journal of Medical Molecular Biology, 2026, 23(3): 258-265.

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活化血小板对内皮细胞凋亡损伤的作用研究

Effect of Platelet Activation on Apoptosis and Damage of Endothelial Cells

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