七叶皂苷钠改善溃疡性结肠炎大鼠肠黏膜屏障损伤机制研究

doi:10.3870/j.issn.1672-8009.2026.02.003

摘要/Abstract

摘要： 目的探讨七叶皂苷钠对葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的溃疡性结肠炎(ulcerative colitis,UC)模型大鼠肠黏膜屏障损伤的影响及机制分析。方法选取65只SD大鼠,分为假手术组,模型组,七叶皂苷钠低、中、高剂量组(2.5、5、10 mg/kg)及阳性对照组(美沙拉嗪,360 mg/kg),每组各10只,使用DSS制备UC大鼠模型。记录药物干预完成后大鼠疾病活动指数(DAI)评分,检测血清D-乳酸(D-LA)、二胺氧化酶(DAO)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)水平;比较各组大鼠结肠溃疡面积、结肠长度,结肠重量及结肠组织中髓过氧化物酶(MPO)、闭锁蛋白(Occludin)、闭锁小带蛋白(ZO-1)、核转录因子相关因子-2(Nrf-2)、抗氧化反应元件(ARE)、血红素加氧酶-1(HO-1)蛋白表达水平,HE染色进行病理评分。结果与模型组相比,七叶皂苷钠中、高剂量干预显著降低UC大鼠DAI评分、病理评分,减轻结肠溃疡、降低结肠重量并增加结肠长度(P<0.05);七叶皂苷钠中、高剂量干预显著降低UC大鼠血清D-LA、DAO、TNF-α、IL-1β、IL-6水平及结肠MPO含量,上调结肠组织中Occludin、ZO-1以及p-Nrf2/Nrf2、ARE、HO-1的蛋白表达(P<0.05)。结论七叶皂苷钠对于改善DSS诱导的UC模型大鼠的肠黏膜屏障损伤具有积极意义,其机制可能与调节炎症和Nrf-2/ARE/HO-1信号通路有关。

关键词: 七叶皂苷钠, 溃疡性结肠炎, 肠黏膜屏障, Nrf-2/ARE/HO-1信号通路

Abstract: Objective To explore the effect of sodium aescinate on intestinal mucosal barrier in rats with ulcerative colitis(UC).Methods A total of 65 SD rats were divided into 6 groups:sham operation group,model group,low-dose,medium-dose and high-dose sodium aescinate groups(2.5,5,10 mg/kg),and positive control group(mesalazine,360 mg/kg),10 rats in each group.UC rat models were induced by DSS.Disease activity index(DAI)scores were recorded after drug intervention.The levels of serum D-lactic acid(D-LA),diamine oxidase(DAO),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6 were detected.The ulcer area,length and weight of colon,expression levels of myeloperoxidase(MPO),Occludin,zonula occludens-1(ZO-1),nuclear factor-erythroid 2-related factor 2(Nrf-2),antioxidant response element(ARE)and heme oxygenase-1(HO-1)proteins in colon tissues were compared among different groups.HE staining was performed and pathological scores were recorded.Results Medium-dose and high-dose sodium aescinate significantly reduced DAI score and pathological score,alleviated colonic ulcers,reduced colon weight and increased colon length(P<0.05).The levels of serum D-LA,DAO,TNF-α,IL-1β,IL-6,and colonic MPO were significantly reduced,and the expression of Occludin,ZO-1,p-Nrf2/Nrf2,ARE and HO-1 proteins in colonic tissues was upregulated in the medium- and high-dose sodium aescinate groups(P<0.05).Conclusion Sodium aescinate can ameliorate intestinal mucosal barrier dysfunction in DSS-induced UC rats,and the mechanisms may be related to the inflammation and Nrf-2/ARE/HO-1 signaling pathway.

Key words: sodium aescinate, ulcerative colitis, intestinal mucosal barrier, Nrf-2/ARE/HO-1 signaling pathway

中图分类号:

R285

张彤, 司梦圆, 林莹, 王丹, 孙吉瑞. 七叶皂苷钠改善溃疡性结肠炎大鼠肠黏膜屏障损伤机制研究[J]. 医学分子生物学杂志, 2026, 23(2): 127-133.

ZHANG Tong, SI Mengyuan, LIN Ying, WANG Dan, SUN Jirui. Mechanism of Sodium Aescinate in Ameliorating Intestinal Mucosal Barrier Dysfunction in Ulcerative Colitis Rats[J]. Journal of Medical Molecular Biology, 2026, 23(2): 127-133.

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