丁酸钠影响脂肪组织浸润Treg和Th17的比例并改善2型糖尿病小鼠胰岛素敏感性

doi:10.3870/j.issn.1672-8009.2025.06.005

医学分子生物学杂志 ›› 2025, Vol. 22 ›› Issue (6): 563-570.doi: 10.3870/j.issn.1672-8009.2025.06.005

丁酸钠影响脂肪组织浸润Treg和Th17的比例并改善2型糖尿病小鼠胰岛素敏感性

王思瑶, 高静, 张春雪, 罗荔, 靳瑾

新疆医科大学第五附属医院内分泌科乌鲁木齐市,830000

收稿日期:2025-01-02 出版日期:2025-11-30 发布日期:2025-12-25
通讯作者: 靳瑾(E-mail:jinjin@xjmu.edu.cn)
基金资助:
新疆医科大学第五附属医院青年科研启航项目(No.XYDWFY-ZR-202308),“天山英才”医药卫生高层次人才培养计划项目(No.TSYC202301B064)

Effect of Sodium Butyrate on Infiltrated Treg and Th17 Proportion in Adipose Tissues and Insulin Sensitivity in Type 2 Diabetic Mice

WANG Siyao, GAO Jing, ZHANG Chunxue, LUO Li, JIN Jin

Department of Endocrinology,the Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi,830000,China

Received:2025-01-02 Online:2025-11-30 Published:2025-12-25
Contact: JIN Jin(E-mail:jinjin@xjmu.edu.cn)
Supported by:
Youth Research Launch Project of the Fifth Affiliated Hospital of Xinjiang Medical University(No.XYDWFY-ZR-202308),the "Tianshan Talents" Medical and Health High Level Talent Training Program Project(No.TSYC202301B064)

摘要/Abstract

摘要： 目的探讨丁酸钠(sodium butyrate)改善2型糖尿病(type 2 diabetes mellitus,T2DM)模型小鼠胰岛素敏感性的免疫学机制。方法饲喂小鼠高脂高糖饮食联合链脲佐菌素诱导建立T2DM小鼠模型。30只雄性C57BL/6小鼠分为3组:对照组,模型组,模型+丁酸钠组,每组10只。测定小鼠葡萄糖耐量试验和胰岛素耐量试验。流式细胞术测定外周血和脂肪组织中浸润的调节性T细胞(regulatory T cells,Treg)和辅助T细胞17(T helper type 17,Th17)比率(%)。蛋白质印迹法测定脂肪组织浸润的Treg和Th17细胞中p-mTOR、mTOR、p-p70S6K和p70S6K的相对表达水平。结果葡萄糖耐量试验结果显示与对照组相比,模型+丁酸钠组在20 min时血糖升高(P<0.05),在后续时间点时血糖无统计学显著性差异(P>0.05)。胰岛素耐量试验结果显示模型+丁酸钠组在所有时间点血糖改变无统计学差异(P>0.05)。与对照组相比,模型组外周血Th17细胞占比(%)和脂肪组织中浸润的Th17细胞占比(%)升高(P<0.05);外周血Treg细胞占比(%)和脂肪组织中浸润的Treg细胞占比(%)降低(P<0.05);脂肪组织中浸润的Treg细胞和Th17细胞中p-mTOR和p-p70S6K表达水平升高(P<0.05)。与模型组相比,模型+丁酸钠组显著逆转了上述指标值(P<0.05)。结论丁酸钠治疗可抑制mTOR/p70S6K信号通路的活化水平,调节外周血和脂肪组织中Treg和Th17细胞数量,改善2型糖尿病小鼠模型胰岛素敏感性。

关键词: 2型糖尿病, 胰岛素敏感性, 丁酸钠, 调节性T细胞, 辅助T细胞17

Abstract: Objective To explore the immunological mechanisms of sodium butyrate on improving Type 2 diabetic mouse model’s insulin sensitivity. Methods Type 2 diabetic mouse model was established by feeding with a high-fat/high-sucrose diet combined with streptozotocin induction.Thirty male C57BL/6 mice were divided into three groups:control group,model group,model+sodium butyrate group,10 mice in each group.The values of glucose tolerance test and insulin tolerance test were measured.The proportion of infiltrated regulatory T cells(Treg)(%)and T helper type 17(Th17)(%)in mice peripheral blood and adipose tissues were measured by flow cytometry.Western blotting was used to determine the relative expression levels of p-mTOR,mTOR,p-p70S6K and p70S6K in infiltrated Treg and Th17 cells in adipose tissues. Results Glucose tolerance test results have shown that compared with control group,in model+sodium butyrate group blood glucose levels increased at 20 min(P<0.05);there was no statistically significant difference in blood glucose at subsequent time points(P>0.05).Insulin tolerance test results have shown that there was no significant difference in blood glucose levels in model+sodium butyrate group at any time points(P>0.05).Compared with those in the control group,the percentage(%)of infiltrating Th17 cells in peripheral blood and adipose tissues was increased in the model group(P<0.05),and the percentage(%)of infiltrating Treg cells was decreased(P<0.05),and the relative expression levels of p-mTOR and p-p70S6K in infiltrated Treg cells and Th17 cells in adipose tissues were increased(P<0.05).Compared with the model group,the model+sodium butyrate group significantly reversed the above indicator values(P<0.05). Conclusion Sodium butyrate treatment could inhibit the activation levels of mTOR/p70S6K signaling pathway,regulate the numbers of Treg cells and Th17 cells in peripheral blood and adipose tissues,and improve insulin sensitivity in type 2 diabetes mice model.

Key words: type 2 diabetes mellitus, insulin sensitivity, sodium butyrate, regulatory T cells, T helper type 17 cells

中图分类号:

R587.1
R576

王思瑶, 高静, 张春雪, 罗荔, 靳瑾. 丁酸钠影响脂肪组织浸润Treg和Th17的比例并改善2型糖尿病小鼠胰岛素敏感性[J]. 医学分子生物学杂志, 2025, 22(6): 563-570.

WANG Siyao, GAO Jing, ZHANG Chunxue, LUO Li, JIN Jin. Effect of Sodium Butyrate on Infiltrated Treg and Th17 Proportion in Adipose Tissues and Insulin Sensitivity in Type 2 Diabetic Mice[J]. Journal of Medical Molecular Biology, 2025, 22(6): 563-570.

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丁酸钠影响脂肪组织浸润Treg和Th17的比例并改善2型糖尿病小鼠胰岛素敏感性

Effect of Sodium Butyrate on Infiltrated Treg and Th17 Proportion in Adipose Tissues and Insulin Sensitivity in Type 2 Diabetic Mice

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