关键词: 苦蒿素, 急性肺损伤, 高迁移率族蛋白 B1, TOLL 受体 4, 核因子 κB, 氧化应激反应

Abstract: Objective To analyze the mechanism of blinin in improving acute lung injury (ALI). Methods A total of 60 neonatal rats were randomly divided into 6 groups, the sham operation group, the model group, the blinin low-, medium- and high-dose groups, and the dexamethasone group. The pulmonary edema was assessed by the blood partial pressure of oxygen (PaO2 ) and the lung wet / dry weight ratio (W/ D). The pathological damage of lung tissues was detected by HE staining. The levels of oxidative stress and inflammation factors in the bronchoalveolar lavage fluid (BALF) were detected by ELISA. The expression levels of the high mobility group box 1 protein (HMGB1) / nuclear factor kappa-B (NF-κB) related proteins in lung tissues were detected by Western blotting. Results The PaO2 was significantly increased in the blinin high-dose group when compared with that in the model group, while the W/ D and lung injury scores, the levels of inflammation and oxidative stress fators in BALF, and the expression levels of HMGB1, TLR4 and p-NF-κB p65 / NF-κB p65 in lung tissues were significantly decreased (P < 0. 05). Conclusion Blinin can improve ALI by enhancing anti-oxidation and anti-inflammatory activity and blocking the HMGB1 / NF-κB signaling pathways.

Key words: blinin, acute lung injury, high mobility group box 1 protein, TOLL receptor 4, nuclear factor kappa-B, oxidative stress response