LncRNA MALAT1 通过调控 miR-181d-5p 对脂多糖诱导的库普弗
细胞增殖活性和炎性因子表达的影响

doi:10.3870/j.issn.1672-8009.2023.05.008

医学分子生物学杂志 ›› 2023, Vol. 20 ›› Issue (5): 426-431.doi: 10.3870/j.issn.1672-8009.2023.05.008

LncRNA MALAT1 通过调控 miR-181d-5p 对脂多糖诱导的库普弗细胞增殖活性和炎性因子表达的影响

¹湖北医药学院附属随州医院随州市中心医院感染科湖北省随州市, 441300 ²湖北文理学院附属医院襄阳市中心医院消化内科湖北省襄阳市, 441021

出版日期:2023-09-30 发布日期:2023-11-13
基金资助:
湖北省卫生和计划生育委员会基金 (No. WJ2017H039)

Effect of LncRNA MALAT1 on Lipopolysaccharide-induced Kupffer Cell Proliferation and Inflammatory Factor Expression via Regulation of miR-181d-5p

¹Department of Infectious Diseases, Suizhou Central Hospital, Suizhou Hospital Affiliated to Hubei University of Medicine, Suizhou, Hubei, 441300, China ²Department of Gastroenterology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei, 441021, China

Online:2023-09-30 Published:2023-11-13

摘要/Abstract

摘要： 目的探讨长链非编码 RNA 肺腺癌转移相关转录本 1 (LncRNA MALAT1) 对脂多糖 (LPS) 诱导的库普弗细胞 (kupffer cells, KCs) 存活和炎性因子表达的影响及其可能作用机制。方法采用 LPS 诱导 KCs 建立细胞损伤模型, 随机分组为对照 ( Con) 组、 LPS 组、 LPS + si-NC 组、 LPS + si-MALAT1 组、 LPS + miR-NC 组、 LPS + miR-181d-5p 组、 LPS + si-MALAT1 + anti-miR-NC 组、 LPS + si-MALAT1 + anti-miR-181d-5p 组; 实时荧光定量聚合酶链式反应 ( qRT-PCR) 检测 LncRNA MALAT1、 miR-181d-5p 的表达量; 细胞计数试剂盒 8 (CCK-8) 法检测细胞增殖活性; 酶联免疫吸附实验 (ELISA) 检测白细胞介素 (IL) -6、 IL-12、肿瘤坏死因子 α (TNF-α) 的水平; 双荧光素酶报告实验检测 LncRNA MALAT1 与 miR-181d-5p 的靶向关系。结果与 Con 组比较, LPS 组 LncRNA MALAT1 的表达量升高 (P< 0. 05), IL-6、 IL-12、 TNF-α 的水平升高 (P< 0. 05), miR-181d-5p 的表达量降低 (P< 0. 05), 细胞增殖活性降低 (P< 0. 05)。与 LPS + si-NC 组比较, LPS + si-MALAT1 组细胞增殖活性升高 ( P < 0. 05), IL-6、 IL-12、 TNF-α 的水平降低 ( P < 0. 05)。 LncRNA MALAT1 可负向调控 miR-181d-5p 的表达; 与 LPS + miR-NC 组比较, LPS + miR-181d-5p 组细胞增殖活性升高 (P< 0. 05), IL-6、 IL-12、 TNF-α 的水平降低 (P< 0. 05)。与 LPS + si-MALAT1 + anti-miR-NC 组比较, LPS + si-MALAT1 + anti-miR-181d-5p 组细胞增殖活性降低 (P< 0. 05), IL-6、 IL-12、 TNF-α 的水平升高 (P< 0. 05)。结论干扰 LncRNA MALAT1 表达可通过促进 miR-181d-5p 表达而促进 LPS 诱导的 KCs 存活及抑制细胞炎性因子表达。

关键词: LncRNA MALAT1, miR-181d-5p, 脂多糖, 库普弗细胞, 炎症因子

Abstract: Objective To investigate the effect of LncRNA MALAT1 on lipopolysaccharide (LPS) -induced survival and expression of inflammatory factors in kupffer cells ( KCs) and its possible mechanism. Methods LPS-induced KCs were used to establish a cell injury model and were randomly divided into 8 groups: Con group, LPS group, LPS + si-NC group, LPS + si-MALAT1 group, LPS + miR-NC group, LPS + miR-181d-5p group, LPS + si-MALAT1 + anti-miR-NC group, LPS + si-MALAT1 + anti-miR-181d-5p group. The expression levels of LncRNA MALAT1 and miR-181d-5p were detected by qRT-PCR. Cell viability was detected by CCK-8 method. The levels of IL-6, IL-12 and TNF-α were detected by ELISA. The targeting relationship between LncRNA MALAT1 and miR-181d-5p was detected by dual luciferase gene reporter assay. Results The expression of LncRNA MALAT1 in the LPS group was increased (P< 0. 05), the levels of IL-6, IL12 and TNF-α were increased (P < 0. 05), while the expression of miR-181d-5p was decreased (P< 0. 05), and the cell viability was decreased (P < 0. 05), when compared with those in the Con group. The cell survival rate in the LPS + si-MALAT1 group was increased (P < 0. 05), while the levels of IL-6, IL-12 and TNF-α were decreased (P< 0. 05), when compared with those in the LPS + si-NC group. The LncRNA MALAT1 negatively regulated the expression of miR-181d-5p. The cell survival rate in the LPS + miR-181d-5p group was increased (P < 0. 05), while the levels of IL-6, IL-12 and TNF-α were decreased (P< 0. 05), when compared with those in the LPS + miR-NC group. The survival rate in the LPS + si-MALAT1 + anti-miR-181d-5p group was decreased (P< 0. 05), while the levels of IL-6, IL-12, TNF-α were increased (P< 0. 05), when compared with those in the LPS + si-MALAT1 + anti-miR-NC group. Conclusion Interfering with the expression of LncRNA MALAT1 could promote the survival of LPS-induced KCs and inhibit the expression of inflammatory factors by promoting the expression of miR-181d-5p.

Key words: LncRNA MALAT1, miR-181d-5p, lipopolysaccharide, kupffer cells, inflammatory factors

中图分类号:

R363

杜娜 , 段少琼 , 宋波 , 李开林 , 颜悦蓉 , 刘悦. LncRNA MALAT1 通过调控 miR-181d-5p 对脂多糖诱导的库普弗细胞增殖活性和炎性因子表达的影响[J]. 医学分子生物学杂志, 2023, 20(5): 426-431.

DU Na , DUAN Shaoqiong , SONG Bo , LI Kailin , YAN Yuerong , LIU Yue . Effect of LncRNA MALAT1 on Lipopolysaccharide-induced Kupffer Cell Proliferation and Inflammatory Factor Expression via Regulation of miR-181d-5p[J]. Journal of Medical Molecular Biology, 2023, 20(5): 426-431.

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LncRNA MALAT1 通过调控 miR-181d-5p 对脂多糖诱导的库普弗细胞增殖活性和炎性因子表达的影响

Effect of LncRNA MALAT1 on Lipopolysaccharide-induced Kupffer Cell Proliferation and Inflammatory Factor Expression via Regulation of miR-181d-5p

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LncRNA MALAT1 通过调控 miR-181d-5p 对脂多糖诱导的库普弗 细胞增殖活性和炎性因子表达的影响

Effect of LncRNA MALAT1 on Lipopolysaccharide-induced Kupffer Cell Proliferation and Inflammatory Factor Expression via Regulation of miR-181d-5p

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