Panx1调控ATP释放对人卵巢癌顺铂耐药SKOV3/DDP细胞凋亡的影响*

doi:10.3870/j.issn.1672-0741.25.07.034

华中科技大学学报（医学版） ›› 2026, Vol. 55 ›› Issue (3): 327-332.doi: 10.3870/j.issn.1672-0741.25.07.034

Panx1调控ATP释放对人卵巢癌顺铂耐药SKOV3/DDP细胞凋亡的影响^*

龙生根, 舒宽勇, 朱其舟, 肖仲清, 章志琴^△

江西省妇幼保健院妇科肿瘤科,南昌 330038

收稿日期:2025-07-15 出版日期:2026-06-15 发布日期:2026-06-17
通讯作者: ^△E-mail:nanchangzzq1010@163.com
作者简介:龙生根,男,1985年生,医学硕士,主治医师,E-mail:nanchanglsg@163.com
基金资助:
^*国家自然科学基金资助项目(No.82360318);江西省卫生健康委科技计划项目(No.202410434);江西省自然科学基金面上项目(No.20232BAB206029)

Effect of Panx1 Regulating ATP Release on Apoptosis of Cisplatin-resistant SKOV3/DDP Cells in Human Ovarian Cancer

Long Shenggen, Shu Kuanyong, Zhu Qizhou et al

Department of Gynecological Oncology, Jiangxi Maternal and Child Health Hospital, Nanchang 330038, China

Received:2025-07-15 Online:2026-06-15 Published:2026-06-17
Contact: ^△E-mail:nanchangzzq1010@163.com

摘要/Abstract

摘要： 目的探讨Panx1在人卵巢癌顺铂耐药株SKOV3/DDP中的表达及其耐药机制。方法采用Western blot检测SKOV3和SKOV3/DDP细胞中Panx1的表达,并利用流式细胞术检测细胞凋亡,分析SKOV3/DDP中Panx1表达及其对细胞凋亡的影响;构建Panx1过表达质粒,转染SKOV3/DDP成功后采用流式细胞术检测细胞凋亡,ELISA检测细胞上清液中ATP含量,分析Panx1介导SKOV3/DDP耐药的作用及机制。结果 SKOV3/DDP中Panx1蛋白表达明显低于SKOV3细胞(0.17±0.05 vs.0.35±0.03,P＜0.01),差异具有统计学意义。顺铂(cis-diamine dichloroplatinum,DDP)可明显抑制SKOV3细胞增殖,呈时间和剂量依赖性。SKOV3和SKOV3/DDP组细胞分别经DDP处理后细胞凋亡率均明显上升(33.96%±0.03%vs.5.01%±0.00%,P＜0.01;14.66%±0.02% vs.4.65%±0.01%,均P＜0.01)。SKOV3/DDP+Panx1过表达质粒组细胞凋亡率较未处理组明显升高(15.69% vs.4.10%,P＜0.01)。SKOV3/DDP+Panx1过表达质粒组细胞外液ATP含量明显高于对照组[(33.57±6.00) vs.(10.65±0.76) ng/mL,P＜0.01]。结论上调Panx1可通过增加SKOV3/DDP细胞ATP释放增强细胞凋亡,卵巢癌顺铂耐药的产生可能与其低表达Panx1介导ATP释放下降而减少细胞凋亡有关。

关键词: 卵巢癌, Panx1, 顺铂耐药, ATP, 细胞凋亡

Abstract: Objective To investigate the expression of Panx1 in cisplatin-resistant SKOV3/DDP strains of human ovarian cancer and its mechanism in drug resistance. Methods Western blotting was used to detect the expression of Panx1 in SKOV3 and SKOV3/DDP.Flow cytometry was used to detect the apoptosis of SKOV3/DDP cells,and the expression of Panx1 in SKOV3/DDP cells and its effect on apoptosis were analyzed.SKOV3/DDP overexpression plasmid was constructed,and SKOV3/DDP cells were transfected successfully.Apoptosis was detected by flow cytometry.ATP content in cell supernatant was detected by ELISA to analyze the role and mechanism of Panx1 mediating SKOV3/DDP resistance. Results The expression of Panx1 protein in SKOV3/DDP was significantly lower than that in SKOV3 group(0.17±0.05 vs.0.35±0.03,P<0.01).The cis-diamine dichloroplatinum(DDP)could significantly inhibit SKOV3 cell proliferation in a time-and dose-dependent manner.Subsequent experiments were conducted at 36 h and 32 μmol/L.The apoptosis rate of SKOV3 and SKOV3/DDP groups was significantly increased after DDP treatment(33.96%±0.03% vs.5.01%±0.00%,P<0.01;14.66%±0.02% vs.4.65%±0.01%,P<0.01).The apoptosis rate of SKOV3/DDP overexpression plasmid group was significantly higher than that of untreated group(15.69% vs.4.10%,P<0.01).ATP content in extracellular fluid of SKOV3/DDP overexpression plasmid group was significantly higher than that of control group(33.57±6.00 vs.10.65±0.76 ng/mL,P<0.01). Conclusion Up-regulation of Panx1 can increase apoptosis of SKOV3/DDP cells by increasing ATP release.Cisplatin resistance of ovarian cancer may be related to the decrease of ATP release mediated by low expression of Panx1.

Key words: ovarian cancer, Panx1, cisplatin resistance, ATP, apoptosis

中图分类号:

R737.31

龙生根, 舒宽勇, 朱其舟, 肖仲清, 章志琴. Panx1调控ATP释放对人卵巢癌顺铂耐药SKOV3/DDP细胞凋亡的影响^*[J]. 华中科技大学学报（医学版）, 2026, 55(3): 327-332.

Long Shenggen, Shu Kuanyong, Zhu Qizhou et al. Effect of Panx1 Regulating ATP Release on Apoptosis of Cisplatin-resistant SKOV3/DDP Cells in Human Ovarian Cancer[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(3): 327-332.

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Panx1调控ATP释放对人卵巢癌顺铂耐药SKOV3/DDP细胞凋亡的影响^*

Effect of Panx1 Regulating ATP Release on Apoptosis of Cisplatin-resistant SKOV3/DDP Cells in Human Ovarian Cancer

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