托珠单抗通过cGAS-STING途径减轻阿霉素诱导的心肌细胞损伤*

doi:10.3870/j.issn.1672-0741.25.07.040

华中科技大学学报（医学版） ›› 2026, Vol. 55 ›› Issue (1): 91-96.doi: 10.3870/j.issn.1672-0741.25.07.040

托珠单抗通过cGAS-STING途径减轻阿霉素诱导的心肌细胞损伤^*

翟福强, 毕秀凤, 安小通, 侯扬^△

济南市第四人民医院心内科, 济南 250031

收稿日期:2025-07-17 出版日期:2026-02-15 发布日期:2026-02-10
通讯作者: ^△E-mail:632844939@qq.com
作者简介:翟福强,男,1988年生,医学硕士,主治医师,E-mail:zzzhaifuqqq@163.com
基金资助:
^*山东省自然科学基金资助项目(No.ZR2024QH521)

Tozumab Alleviates Doxorubicin-induced Myocardial Cell Injury through the cGAS STING Pathway

Zhai Fuqiang, Bi Xiufeng, An Xiaotong et al

Department of Cardiology, The Fourth People’s Hospital of Ji’nan, Ji’nan 250031, China

Received:2025-07-17 Online:2026-02-15 Published:2026-02-10
Contact: ^△E-mail:632844939@qq.com

摘要/Abstract

摘要： 目的旨在探究托珠单抗对阿霉素诱导的急性心肌损伤的保护作用。方法先用不同浓度的托珠单抗(1、3和5 mg/mL)处理AC16心肌细胞,再用阿霉素(1 μmol/L)诱导构建急性心肌损伤细胞模型。采用流式细胞术检测细胞凋亡率,采用CCK-8法评估细胞活力。采用JC-1染色分析线粒体膜电位。采用DCFH2-DA探针检测活性氧(ROS)水平。通过qRT-PCR和Western blot评估cGAS、STING和CXCL10的表达。结果在阿霉素诱导细胞毒性的情况下,托珠单抗显着增加了AC16细胞活力(P<0.01),并降低了早期和晚期细胞凋亡率(均P<0.01)。另外,托珠单抗降低了细胞内ROS水平(P<0.01),并下调了cGAS、STING和CXCL10的表达。结论托珠单抗通过调节cGAS-STING通路对阿霉素诱导的心肌损伤具有保护作用,凸显了其作为治疗剂提高化疗期间心脏安全的潜力。

关键词: 托珠单抗, 急性心肌损伤, 阿霉素, 细胞凋亡

Abstract: Objective This study aimed to explore the protective effect of tocilizumab on acute myocardial injury induced by doxorubicin. Methods Human AC16 cardiomyocytes were treated with different concentrations of tocilizumab(1,3,and 5 μg/mL)before exposure to doxorubicin(1 μmol/L).Cell viability was assessed using the CCK-8 assay,while apoptosis was measured by flow cytometry.The ROS level was assessed with a DCFH2-DA probe.JC-1 staining was used to analyze the mitochondrial membrane potential.The expression levels of CXCL10,STING,and cGAS were detected through Western blotting and qRT-PCR. Results In the context of the cytotoxicity induced by doxorubicin,tocilizumab significantly increased AC16 cell viability(P<0.01)and reduced early and late apoptosis rates(P<0.01).In addition,tocilizumab reduced the ROS level(P<0.01)and downregulated the expression of cGAS,STING and CXCL10. Conclusion Tocilizumab has a cardioprotective effect on doxorubicin-induced myocardial injury by modulating the cGAS-STING pathway,highlighting its potential as a therapeutic agent to improve cardiac safety during chemotherapy.

Key words: tocilizumab, acute myocardial injury, doxorubicin, apoptosis

中图分类号:

R542.2

翟福强, 毕秀凤, 安小通, 侯扬. 托珠单抗通过cGAS-STING途径减轻阿霉素诱导的心肌细胞损伤^*[J]. 华中科技大学学报（医学版）, 2026, 55(1): 91-96.

Zhai Fuqiang, Bi Xiufeng, An Xiaotong et al. Tozumab Alleviates Doxorubicin-induced Myocardial Cell Injury through the cGAS STING Pathway[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(1): 91-96.

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托珠单抗通过cGAS-STING途径减轻阿霉素诱导的心肌细胞损伤^*

Tozumab Alleviates Doxorubicin-induced Myocardial Cell Injury through the cGAS STING Pathway

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