基于网络药理学和实验研究探讨仙鹤草治疗支气管哮喘的作用机制*

doi:10.3870/j.issn.1672-0741.24.08.018

华中科技大学学报（医学版） ›› 2026, Vol. 55 ›› Issue (1): 45-52.doi: 10.3870/j.issn.1672-0741.24.08.018

基于网络药理学和实验研究探讨仙鹤草治疗支气管哮喘的作用机制^*

高国财¹, 郑宏^2,3, 潘丹萍¹, 白朝辉⁴, 韩雪^1△

郑州大学附属儿童医院 ¹中医科 ⁴药学部, 郑州 450018
²河南中医药大学第一附属医院儿科医院儿科, 郑州 450046
³河南中医药大学儿科医学院, 郑州 450046

收稿日期:2024-08-21 出版日期:2026-02-15 发布日期:2026-02-10
通讯作者: ^△E-mail:hxue1016@163.com
作者简介:高国财,男,1987年生,医学硕士,副主任中医师,E-mail:ggci001@163.com
基金资助:
^*国家中医药管理局科技项目(No.GZY-KJS-2022-038-3);河南省中医药科学研究专项课题(No.20-21ZY2094,No.2023ZY2168);河南省特色骨干学科中医学学科建设项目(No.STG-ZYX04-202137)

The Mechanism of Agrimonia pilosa in the Treatment of Bronchial Asthma via Network Pharmacology and Experimental Study

Gao Guocai¹, Zheng Hong^2,3, Pan Danping¹ et al

¹Department of Traditional Chinese Medicine, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450018, China
²Department of Pediatrics, Pediatric Hospital, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450046, China
³School of Pediatric Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China

Received:2024-08-21 Online:2026-02-15 Published:2026-02-10
Contact: ^△E-mail:hxue1016@163.com

摘要/Abstract

摘要： 目的采用网络药理学和分子对接探讨仙鹤草治疗支气管哮喘(bronchial asthma,BA)的作用机制,并用动物实验予以验证。方法基于TCMSP、OMIM、DisGenet、GeneCards等多种数据库,获得仙鹤草治疗BA的关键成分及核心靶点。应用String及Cytoscape构建作用靶点的PPI网络,筛选出核心作用靶点,并进行GO功能注释和KEGG通路富集分析。采用4%卵清蛋白致敏建立BA大鼠模型,ELISA法检测支气管肺泡灌洗液中IL-4、IL-5、IL-6和IFN-γ水平,Western blot法检测肺组织p38 MAPK、P-p38 MAPK、NF-κB p65、NF-κB P-p65蛋白表达水平,研究仙鹤草对BA大鼠的作用,验证相关靶点通路。结果共获得仙鹤草活性成分5个,仙鹤草治疗BA的交集潜在作用靶点120个,炎症相关核心靶点5个,涉及159条信号通路。分子对接验证结果表明,槲皮素与炎症相关核心靶点结合稳定、亲和力良好。BA模型动物实验结果表明,仙鹤草可降低IL-4、IL-5、IL-6、IL-13细胞因子水平,升高IFN-γ水平,Western blot结果显示,仙鹤草可下调P-p38 MAPK、NF-κBP-p65水平。结论仙鹤草可能通过抑制p38 MAPK/NF-κB信号通路,减少Th2细胞因子生成,减轻炎症反应,从而发挥对BA的治疗作用。

关键词: 仙鹤草, 支气管哮喘, 网络药理学, 分子对接, 炎症

Abstract: Objective This study aimed to investigate the therapeutic mechanism of Agrimonia pilosa in the treatment of bronchial asthma(BA) via network pharmacology and molecular docking techniques,followed by validation through animal experiments. Methods The key bioactive components and core therapeutic targets of Agrimonia pilosa for treating BA were identified from multiple databases,including TCMSP,OMIM,DisGenet,and GeneCards.The protein-protein interaction(PPI)network of these targets was subsequently constructed via STRING and Cytoscape,and the core targets were selected for Gene Ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.A BA rat model was established by sensitizing the rats with 4% ovalbumin.The levels of IL-4,IL-5,IL-6,and IFN-γ in bronchoalveolar lavage fluid were determined via ELISA,while the protein expression levels of p38 MAPK,P-p38 MAPK,NF-κB p65,and NF-κB P-p65 in lung tissue were assessed via Western blotting to evaluate the effects of Agrimonia pilosa on BA rats and verify the associated signaling pathways. Results A total of five active components of Agrimonia pilosa were identified,along with 120 intersecting potential therapeutic targets for BA.Among these,five inflammation-related core targets,which were found to be involved in 159 signaling pathways,were highlighted.Molecular docking analysis revealed that quercetin displayed stable binding and high affinity with the identified inflammation-related core targets.The results of animal experiments using the BA model revealed that Agrimonia pilosa decreased the levels of the cytokines IL-4,IL-5,IL-6,and IL-13 while increasing the IFN-γ level.Moreover,Western blot analysis revealed that Agrimonia pilosa downregulated the protein expression of P-p38 MAPK and NF-κB P-p65. Conclusion Agrimonia pilosa may exert its effects by inhibiting the p38 MAPK/NF-κB signaling pathway,thereby reducing Th2 cytokine production and alleviating inflammatory responses,which may be a mechanism of Agrimonia pilosa in the treatment of BA.

Key words: Agrimonia pilosa, bronchial asthma, network pharmacology, molecular docking, inflammation

中图分类号:

R562.25

高国财, 郑宏, 潘丹萍, 白朝辉, 韩雪. 基于网络药理学和实验研究探讨仙鹤草治疗支气管哮喘的作用机制^*[J]. 华中科技大学学报（医学版）, 2026, 55(1): 45-52.

Gao Guocai, Zheng Hong, Pan Danping et al. The Mechanism of Agrimonia pilosa in the Treatment of Bronchial Asthma via Network Pharmacology and Experimental Study[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(1): 45-52.

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基于网络药理学和实验研究探讨仙鹤草治疗支气管哮喘的作用机制^*

The Mechanism of Agrimonia pilosa in the Treatment of Bronchial Asthma via Network Pharmacology and Experimental Study

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