Abstract: Objective To explore the effect of rifampicin on miR-28-5p/nuclear factor-erythroid 2-related factor 2(Nrf2)signaling and liver cell injury. Methods L02 cells were divided into 6 groups:control group,low-,medium- ,and high-dose rifampicin groups,miR NC group,and miR-28-5p inhibitor group.The expression level of miR-28-5p,cell viability,apoptosis rate,inflammatory factor levels,superoxide dismutase(SOD),malondialdehyde(MDA),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)in each group of L02 cells were detected.Meanwhile,the targeting relationship between miR-28-5p and Nrf2 in L02 cells was analyzed. Results The expression level of miR-28-5p in L02 cells were increased with the increase of rifampicin concentration.Compared with those in the control group,the viability of L02 cells in the low-,medium-,and high-dose rifampicin groups,miR NC group,and miR-28-5p inhibitor group was decreased,the apoptosis rate was increased,the levels of inflammatory factors,MDA,AST,and ALT were increased,and the levels of SOD and Nrf2 were decreased.Compared with those in the miR NC group,the viability of L02 cells in the miR-28-5p inhibitor group was increased,the apoptosis rate was decreased,the levels of inflammatory factors,MDA,AST,and ALT were decreased,and the levels of SOD and Nrf2 were increased.miR-28-5p could targetly inhibit the expression of Nrf2. Conclusion Rifampicin can promote liver cell injury by miR-28-5p and inhibition of Nrf2.

Key words: nuclear factor-erythroid 2-related factor 2, rifampicin, miR-28-5p, apoptosis, hepatocyte injury