Improvement of Paraquat-induced Lung Injury and Fibrosis by Total Glucosides of Paeonia in Rats

doi:10.3870/j.issn.1672-8009.2026.04.010

Abstract

Abstract: Objective To explore the effects of total glucosides of paeony(TGP)on oxidative stress and lung fibrosis in rats with paraquat(PQ)-induced lung injury. Methods Intraperitoneal injection of PQ was performed to induce lung injury in rats,then those rats were intervened with different doses of TGP.The pathological changes of lung tissues,oxidative stress,levels of inflammatory factors,mRNA levels of fibrosis-related factors and expressions of TGF-β1/Smad3 signaling pathway proteins were observed.RLE-6TN cells were treated with PQ and intervened with TGP.The expression levels of TGF-β1/Smad3 pathway related factors in cells were detected. Results After TGP intervention,the lung injury,the levels of MDA and HYP,the levels of serum TNF-α,IL-6 and IL-1β,the mRNA expression levels of α-SMA,Collagen Ⅲ and TIMP-1,and the expression levels of TGF-β1 and p-Smad3/Smad3 were decreased,while the levels of SOD and CAT,and the ratio of p-Smad7/Smad7 were increased,in a dose-dependent manner.After TGP intervention,the expression level of p-Smad7 in RLE-6TN cells was increased,while the expression levels of TGF-β1 and p-Smad3 were decreased. Conclusion TGP can improve oxidative stress and lung fibrosis in PQ rats.The mechanism may be related to TGF-β1/Smad3 signaling pathways.

Key words: paraquat, lung injury, total glucosides of paeonia, oxidative stress, lung fibrosis

CLC Number:

R322.3
R963

LIU Mei, SI Yongwang , LIU Li , CUI Chunye , WANG Dan , LUO Yingying. Improvement of Paraquat-induced Lung Injury and Fibrosis by Total Glucosides of Paeonia in Rats[J]. Journal of Medical Molecular Biology, 2026, 23(4): 437-444.

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