LncRNA MIR100HG Promotes Epithelial-mesenchymal Transition in Triple-negative Breast Cancer MDA-MB-231 Cells

doi:10.3870/j.issn.1672-8009.2025.06.010

Abstract

Abstract: Objective To investigate the effect of long non-coding RNA(lncRNA)MIR100HG on epithelial-mesenchymal transition(EMT)of triple-negative breast cancer MDA-MB-231 cells. Methods MIR100HG was overexpressed or inhibited in the MDA-MB-231 cells,cell proliferation was measured by MTS assay,cell apoptosis rate was analyzed by flow cytometry,and cell migration and invasion were determined by Transwell assay.Western blotting was used to detect the regulatory effect of MIR100HG on epithelial-mesenchymal transition related markers. Results Overexpression of MIR100HG promoted the proliferation,migration and invasion of MDA-MB-231 cells,reduced cell apoptosis,downregulated the expression of epithelial markers and upregulated the expression of mesenchymal markers; Inhibition of MIR100HG produced opposite results. Conclusion Overexpression of LncRNA MIR100HG can promote the proliferation,migration and invasion of MDA-MB-231 cells,reduce the apoptosis of MDA-MB-231 cells,and induce the epithelial-mesenchymal transition of MDA-MB-231 cells.

Key words: long non-coding RNA, MIR100HG, epithelial-mesenchymal transition, MDA-MB-231

CLC Number:

R363

LI Changchun, YAN Wenyue, LIU Genxiang, ZHOU Guangjun. LncRNA MIR100HG Promotes Epithelial-mesenchymal Transition in Triple-negative Breast Cancer MDA-MB-231 Cells[J]. Journal of Medical Molecular Biology, 2025, 22(6): 601-606.

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