Abstract: Objective Explore the effect of Pterostilbene on the biological behavior of humantongue squamous cell carcinoma CAL-27 cells. Methods CAL-27 cells were divided into 2 groups:control group and experimental group. Cells in the experimental group were treated with Pterostilbene(10, 20, 40 μmol / L). CCK-8 assay, colony formation assay and EdU staining were used to measure the proliferation of CAL-27 cells. Flow cytometry was used to determine the apoptosis of CAL-27cells. Wound-healing and Transwell assay were used to detect the migration and invasion ability of CAL-27 cells. Western blotting was used to detect the expression levels of epithelial-mesenchymaltransition (EMT) -related proteins in CAL-27 cells. Xenograft model was used to verify the effect ofpterostilbene on tumor in vivo. Results The proliferation, migration and invasion abilities of CAL-27 cells were weakened, the apoptosis rate was increased, the expression levels of Bax, E-cadherin and Claudin1 proteins in the cells were increased, and the expression levels of Bcl-2, N-cadherin, Vimentin and Snail proteins were decreased after 24 h treatment with 20 and 40 μmol / Lpterostilbene when compared with those in the control group ( P < 0. 05). After treatment with 30mg / kg pterostilbene, the weight and volume of transplanted tumors in nude mice decreased significantly, the number of Ki67 and Vimentin positive cells decreased significantly, and the number of TUNEL positive cells increased significantly ( P < 0. 05). Conclusion Pterostilbene can inhibitCAL-27 cells proliferation, migration, invasion and EMT and induce its apoptosis.

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