Abstract: Objective To explore the effect and mechanism of TONCS _ 00279227(Linc279227) on renal injury in diabetes nephropathy mice. Methods Mice were divided into aControl group and a Model group. After successful modeling of db / db mice in the Model group, the mice kidney tissues were taken. qRT-PCR was used to detect the expression levels of Linc279227, PINK1, and Parkin in mice kidney tissues. Western blotting was used to detect the protein expression levels of LC3-Ⅱ , P62, PINK1, and Parkin in mice kidney tissues. Mouse renal podocyte MPC5 cells were divided into 3 groups: Vector group, Linc27922 group, and Linc27922 group. CCK8 and flow cytometry were used to detect cell proliferation and apoptosis. Western blotting was used to detect the expression levels of LC3-Ⅱ , P62, PINK1, and Parkin proteins in eachgroup of cells. Results Compared with those in the Control group, the expression levels of Linc279227 and P62 protein in the renal tissues of the model group mice were significantly upregulated (P< 0. 01), while the expression levels of PINK1 and Parkin mRNA and proteins and the LC3-Ⅱ protein were significantly downregulated (P < 0. 01) . Compared with those in the Vectorgroup, the mRNA and protein expression levels of PINK1 and Parkin and the protein expression level of LC3-Ⅱ in MPC5 cells of the Linc279227 group were significantly downregulated (P < 0. 01), and the expression level of P62 was significantly upregulated (P < 0. 01) . Compared with those inthe Linc279227 group, the LC3-Ⅱ expression level of MPC5 cells in the Linc27922 + FCCP group wasupregulated (P < 0. 01), the expression level of P62 was significantly downregulated (P < 0. 01), the cell proliferation ability was significantly increased (P<0. 05), and the cell apoptosis was significantly reduced (P < 0. 01) . Conclusion Linc279227 can mediate mitochondrial autophagy of nephropodocytes by regulating the PINK1 / Parkin pathway to affect renal injury in diabetes nephropathy mice.

Key words: diabetes nephropathy, Linc279227, autophagy, PINK1 / Parkin pathway