1β-Hydroxyalantolactone Ameliorates Hepatic Injury in Rats with Nonalcoholic Steatohepatitis via AMPK/SREBP-1c Signaling Pathway

doi:10.3870/j.issn.1672-8009.2026.03.009

Abstract

Abstract: Objective To investigate the effect of 1β-hydroxyalantolactone(1β-HA)on hepatic injury in rats with nonalcoholic steatohepatitis(NASH)through the AMPK/SREBP-1c signaling pathway. Methods A rat model of NASH was established by feeding with high-fat diet.The rats were administered 1β-HA at doses of 12.5,25.0,and 50.0 mg/kg by oral gavage for 4 weeks.Serum levels of HDL,TG,ALT,and AST were measured.Hepatic histopathology was assessed.The protein expression levels of α-SMA,MMP-1,TIMP-1,AMPK,and SREBP-1c were detected.The levels of IL-6,TNF-α,and IL-1β,as well as SOD activity and MDA concentration,were evaluated. Results Compared with the model group,the 1β-HA treatment groups showed significantly increased HDL levels and decreased TG,AST,and ALT levels(P<0.05);hepatic steatosis,inflammatory infiltration,and fibrosis were alleviated;the expression levels of α-SMA,TIMP-1,and SREBP-1c were down-regulated,while those of MMP-1 and AMPK were up-regulated(P<0.05);the levels of IL-6,TNF-α,and IL-1β were reduced;SOD activity was elevated,and MDA concentration was decreased(P<0.05). Conclusion 1β-HA can ameliorate hepatic injury in NASH by activating AMPK and inhibiting the expression of SREBP-1c.

Key words: 1β-hydroxyalantolactone, nonalcoholic steatohepatitis, steatosis, liver fibrosis

CLC Number:

R285.5

CHANG Jian, ZHANG Xiaxia, TIAN Liying, YUAN Yunfei, LI Sen, SONG Meifang, WU Linxia. 1β-Hydroxyalantolactone Ameliorates Hepatic Injury in Rats with Nonalcoholic Steatohepatitis via AMPK/SREBP-1c Signaling Pathway[J]. Journal of Medical Molecular Biology, 2026, 23(3): 302-307.

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